Structure-Guided Recombination Creates an Artificial Family of Cytochromes P450 [PDF]
, 2006 Creating artificial protein families affords new opportunities to explore the determinants of structure and biological function free from many of the constraints of natural selection.
Arnold, Frances H. +5 more
core +1 more source
Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella [PDF]
, 2017 Aiming to combine the flexibility of Brucella lumazine synthase (BLS) to adapt different protein domains in a decameric structure and the capacity of BLS and flagellin to enhance the immunogenicity of peptides that are linked to their structure, we ...
Berguer, Paula Mercedes +11 more
core +3 more sources
INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY [PDF]
Biotechnologia Acta, 2017 Obtaining single-chain variable fragments (scFv) of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product.
O.V. Galkin +3 more
doaj +1 more source
Preparation of hexagonal GeO2 particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras [PDF]
, 2014 We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG)
Avanzato +47 more
core +1 more source
AMB Express, 2018 Sortases are enzymes mostly found in Gram-positive bacteria which cleave proteins site-specifically. This feature makes them a promising tool in molecular biology and biotechnology.
Hamidreza Fasehee +3 more
doaj +1 more source
Regions of the T cell receptor alpha and beta chains that are responsible for interactions with CD3. [PDF]
, 1991 The T cell antigen receptor consists of the Ti alpha/beta heterodimer which recognizes antigen, and the associated CD3 chains, thought to be involved in signal transduction.
Tan, L, Turner, J, Weiss, A
core +1 more source
Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]
, 2012 Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi +9 more
core +5 more sources
A synthetic biology approach for a vaccine platform against known and newly emerging serotypes of bluetongue virus [PDF]
, 2014 Bluetongue is one of the major infectious diseases of ruminants and is caused by Bluetongue virus (BTV), an arbovirus existing in nature in at least 26 distinct serotypes. Here, we describe the development of a vaccine platform for BTV.
Brunet, Silvie +6 more
core +2 more sources
Chimeric Protein-LG and Protein-LAG sandwich ELISA v1 [PDF]
, 2021 This ELISA is used to study the interactions between protein-LG (SpLG) and protein-LAG (PLAG) with different immunoglobulin preparations from mammals and avian species.
openaire +6 more sources
Chimeric mitochondrial peptides from contiguous regular and swinger RNA
Computational and Structural Biotechnology Journal, 2016 Previous mass spectrometry analyses described human mitochondrial peptides entirely translated from swinger RNAs, RNAs where polymerization systematically exchanged nucleotides. Exchanges follow one among 23 bijective transformation rules, nine symmetric
Hervé Seligmann
doaj +1 more source