Results 121 to 130 of about 83,358 (225)

Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B‐Like 2 (PLBL2) Captured From a Monoclonal Antibody

Biotechnology and Bioengineering, Volume 123, Issue 2, Page 406-419, February 2026.
A platform approach to the affinity capture and enrichment of host cell proteins to enable their subsequent deep characterization and elucidation of variants or proteoforms (e.g., molecular size variants, charge variants, glycoforms, etc.). ABSTRACT Removing host cell proteins (HCPs) during biotherapeutic manufacturing is essential to ensure patient ...
Michael E. Dolan, Lei (Leo) Wang, Alexander Tedeschi, Yan Wang, Christopher Barton, Sheldon F. Oppenheim, Zhaohui Sunny Zhou   +6 more
wiley   +1 more source

Comparative Analysis of HEK293 Genomic Variability

Biotechnology and Bioengineering, Volume 123, Issue 2, Page 436-448, February 2026.
An investigation of how HEK293‐derived cell lines adapt genetically to different culture conditions and environmental pressures: despite distinct phenotypes and cultivation histories, comparative whole‐genome analyses of established HEK293 variants as well as newly suspension‐adapted cells revealed a shared, common set of mutations linked to cell ...
Georg Smesnik, Nikolaus Virgolini, Maria Toth, Astrid Dürauer, Nicole Borth   +4 more
wiley   +1 more source

Performance Comparison between Semi‐Automated and Manual Cell Counting for Animal Cell Culture

Chemie Ingenieur Technik, Volume 98, Issue 1-2, Page 57-63, February 2026.
Following ISO 20391‐2:2019 guidelines, manual and semi‐automated cell counting performances were compared using validation parameters from ICH Q2(R1). Specificity, linearity, and repeatability served as key validation parameters in the evaluation of total cell density and cell viability of CHO‐K1 cells using trypan blue exclusion staining.
Selina Uhlendorff, Ulrich Odefey, Björn Frahm, Miriam Pein‐Hackelbusch   +3 more
wiley   +1 more source

Exploring CPP Enhanced Hypoxia Targeting: An Approach Using Radiolabeled 2‐nitroimidazole TAT Conjugate

Drug Development Research, Volume 87, Issue 1, February 2026.
ABSTRACT Mediated by their protein transduction domain, cell‐penetrating peptides (CPPs) have been widely explored for facilitating intracellular delivery of drugs and small molecules. In this study, a radiolabeled, 2‐nitroimidazole conjugated TAT (TAT ‐ transactivator of transcription) peptide was prepared and evaluated for targeting tumor hypoxia ...
Sweety Mittal, Akanksha Jain, Ashwini Babu, Chandan Kumar, Madhava B. Mallia   +4 more
wiley   +1 more source

Tetravalent antibodies are more potent and efficacious erythropoiesis‐stimulating agents than erythropoietin in vivo

Protein Science, Volume 35, Issue 2, February 2026.
Abstract Recent studies have shown that tetravalent antibodies are potent and efficacious agonists of the erythropoietin (EPO) receptor (EPOR) both in vitro and in vivo. To identify antibody‐based erythropoiesis‐stimulating agents (ESAs) with therapeutic potential, we evaluated various tetravalent antibody formats for EPOR agonism and key biophysical ...
Jarrett J. Adams, Levi L. Blazer, Jacky Chung, Minoo Karimi, Taylor Davidson, Bailey Blair, Carlos Waddle, Craig A. Hokanson, Heather A. Bruce, Alexander U. Singer, Eva‐Maria Tombak, Kiira Gildemann, Nele Tamberg, Kaja Kiiver, Mart Ustav Jr, Yue Ma, Luigi Colombo, Lily Jun‐Shen Huang, Stephen W. Michnick, Orson W. Moe, Sachdev S. Sidhu   +20 more
wiley   +1 more source

Zinc‐Mediated Lysosomal Destabilization Links Mitochondrial Damage to Neuronal Death in a Cellular MPP+ Model of Parkinson's Disease

Journal of Neurochemistry, Volume 170, Issue 2, February 2026.
When mitochondria are damaged by MPP+, they produce excess reactive oxygen species (ROS) and release zinc (Zn2+) inside the cell. This surge in zinc overwhelms lysosomes—the cell's major zinc‐buffering organelles—causing their membranes to break down. Leaking lysosomal enzymes then trigger neuronal death. Treatments that reduce ROS, bind zinc, or block
Hyun‐Seung Lee, Sun‐Ah Kang, Jae‐Won Eom, Min Seong Kim, Ji‐Soo Kim, Yang‐Hee Kim   +5 more
wiley   +1 more source

Peer review of the pesticide risk assessment of the active substance paraffin oil (CAS 72623‐86‐0, chain lengths C19–C28)

EFSA Journal, Volume 24, Issue 2, February 2026.
Abstract The conclusions of the European Food Safety Authority (EFSA) following the peer review of the initial risk assessments carried out by the competent authorities of the rapporteur Member State, Greece, and co‐rapporteur Member State, France, for the pesticide active substance paraffin oil (CAS 72623‐86‐0) are reported.
European Food Safety Authority (EFSA), Fernando Álvarez, Maria Arena, Domenica Auteri, Sofia Batista Leite, Amarela Becirovic, Marco Binaglia, Anna Federica Castoldi, Arianna Chiusolo, Angelo Colagiorgi, Mathilde Colas, Federica Crivellente, Chloe De Lentdecker, Isabella De Magistris, Mark Egsmose, Gabriella Fait, Franco Ferilli, German Giner Santonja, Varvara Gouliarmou, Katrin Halling, Alessio Ippolito, Frederique Istace, Dimitra Kardassi, Aude Kienzler, Anna Lanzoni, Roberto Lava, Renata Leuschner, Alberto Linguadoca, Mariano Lopez Romano, Jochem Louisse, Christopher Lythgo, Oriol Magrans, Iris Mangas, Andrea Mioč, Ileana Miron, Tunde Molnar, Laura Padovani, Vincenzo Padricello, Martina Panzarea, Juan Manuel Parra Morte, Simone Rizzuto, Miguel Santos, Rositsa Serafimova, Rachel Sharp, Csaba Szentes, Anne Theobald, Manuela Tiramani, Giorgia Vianello, Laura Villamar‐Bouza   +48 more
wiley   +1 more source

Fantastic genes and where to find them expressed in CHO

Computational and Structural Biotechnology Journal
The transcriptome of Chinese hamster ovary (CHO) cells plays a crucial role in determining cellular characteristics that are essential for biopharmaceutical applications.
Markus Riedl, Caterina Ruggeri, Nicolas Marx, Nicole Borth   +3 more
doaj   +1 more source

CIRP Expression on Growth and Productivity of CHO Cells [PDF]

, 2006
Mammalian cell culture is typically operated at the physiological temperature of 37°C. Low temperature cell culture at 30-33°C, in particular for CHO cells, increased the specific productivity of many recombinant proteins amongst many other benefits ...
Tan, Hong-Kiat, Wang, Daniel I.C., Yap, Miranda G.S.   +2 more
core  

Protein overproduction alters exosome secretion in Chinese hamster ovary cells. [PDF]

Anal Bioanal Chem, 2023
Steć A, Targońska M, Karkosińska E, Słowik M, Płoska A, Kalinowski L, Wielgomas B, Waleron K, Jasiecki J, Dziomba S.   +9 more
europepmc   +1 more source