Results 191 to 200 of about 83,358 (225)

Conserved MicroRNAs in Chinese hamster ovary cell lines

Biotechnology and Bioengineering, 2010
AbstractMicroRNAs (miRNAs), a class of short (20–24 nt) non‐coding RNAs that direct post‐transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high‐throughput sequencing. Six cDNA libraries of small
Kathryn C, Johnson, Nitya M, Jacob, Peter Morin, Nissom, Matthias, Hackl, Lim Hseuh, Lee, Miranda, Yap, Wei-Shou, Hu   +6 more
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CHINESE HAMSTER OVARY CELL LINE

2012
The present invention is related to a Chinese hamster ovary cell line as deposited with the European Collection of Cell Cultures (ECACC) under accession number 10090201; use of the cell line for the production of a recombinant polypeptide; a kit comprising the cell line; and methods for the production of recombinant polypeptide.
HATTON DIANE, FIELD RAY, FORREST-OWEN WYN, CROOK VICTORIA, MEDIMMUNE LTD   +4 more
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Chinese hamster ovary cell mutants affecting cholesterol metabolism

Current Opinion in Lipidology, 1997
The approach of somatic cell and molecular genetics for the study of intracellular regulation of cholesterol metabolism has blossomed in recent years. This review lists all the Chinese hamster ovary cell mutants involved in cholesterol metabolism.
Chang, T., Hasan, M., Chin, J., Chang, C., Spillane, D., Chen, J.   +5 more
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Acetylation of spermidine in Chinese hamster ovary cells

Biochemical and Biophysical Research Communications, 1980
Summary Incubation of Chinese hamster ovary (CHO) cells with [14C]spermidine resulted in a linear uptake of radiolabel over a 24-h period. Qualitative analysis of the [14C]metabolites present in the cells demonstrated the formation of both N1- and N8-acetylspermidine.
C E, Prussak, D H, Russell
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Hyperthermic Radiosensitization of Thermotolerant Chinese Hamster Ovary Cells

Radiation Research, 1986
Synchronous G1 cells were given a priming dose of heat (45.5 degrees C for 15 min) and then heated and irradiated 6-120 h later. Compared to heat radiosensitization for cells irradiated 10 min after the priming heat dose (thermal enhancement ratio, TER of 2.6 for a 10-fold reduction in survival), heat radiosensitization 18-24 h after the priming heat ...
P K, Holahan, R S, Wong, L L, Thompson, W C, Dewey   +3 more
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Developing genomic platforms for Chinese hamster ovary cells

Biotechnology Advances, 2009
Chinese hamster ovary (CHO) cells are widely used in recombinant protein production, yet despite their importance in bioprocessing, few genomic resources have been developed for this cell line. Over the past several years, we have made considerable progress in the development of genomic tools for CHO.
Anne, Kantardjieff, Peter Morin, Nissom, Song Hui, Chuah, Faraaz, Yusufi, Nitya M, Jacob, Bhanu Chandra, Mulukutla, Miranda, Yap, Wei-Shou, Hu   +7 more
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Nucleoside kinase activities of Chinese hamster ovary cells

Biochimica et Biophysica Acta (BBA) - General Subjects, 1983
Chinese hamster ovary (CHO) cells and appropriate drug-resistant mutants derived from them have been analyzed for nucleoside kinase activities relevant to the phosphorylation of adenosine, deoxyadenosine, deoxyguanosine and deoxycytidine and for resistance to a variety of nucleoside analogs.
P P, Saunders, M M, Lai
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Metabolomics of Chinese Hamster Ovary Cells

Increasing demand of protein biotherapeutics produced using Chinese hamster ovary (CHO) cell lines necessitates improvement in the production yield of the bioprocess. Various cell engineering, improved media formulation and process-design based approaches utilizing the power of OMICS technologies, specifically, genomics and proteomics, have been ...
Rita, Singh, Sneh, Bajpai, Amardeep, Singh, Phulwanti, Sharma, Yashwant, Kumar, Niraj, Kumar   +5 more
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Biallelic Gene Knockouts in Chinese Hamster Ovary Cells

2008
Chinese hamster ovary (CHO) cells are the most common host cells and are widely used in the manufacture of approved recombinant therapeutics. They represent a major new class of universal hosts in biopharmaceutical production. However, there remains room for improvement to create more ideal host cells that can add greater value to therapeutic ...
Naoko, Yamane-Ohnuki, Kazuya, Yamano, Mitsuo, Satoh   +2 more
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Optical binding of Chinese hamster ovary cells

SPIE Proceedings, 2005
Optical binding may arise due to interplay between light scattering and refraction creating equilibrium positions for particles in a self-consistent manner. Binding is observed for the first time in biological cells within a dual beam fiber trap.
N. K. Metzger, P. R. T. Jess, L. Paterson, E. M. Wright, K. Dholakia   +4 more
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