Optimization of human interferon beta protein expression in Chinese hamster ovary cells [PDF]
Journal of Shahrekord University of Medical Sciences, 2021 Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ...
Nasrin Xodadadi +3 more
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Variation in the Thyrotropic Activity of Human Chorionic Gonadotropin in Chinese Hamster Ovary Cells Arises from Differential Expression of the Human Thyrotropin Receptor and Microheterogeneity of the Hormone. [PDF]
, 1995 The role of hCG as a stimulator of the human thyroid has been a subject of controversy, because discrepant results have been obtained in different in vitro assays.
Amir, S.M. +4 more
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Chinese hamster ovary cells replicate adenovirus deoxyribonucleic acid. [PDF]
Molecular and Cellular Biology, 1981 Chinese hamster ovary (CHO) cells infected with adenovirus type 2 (Ad2) produced amounts of viral deoxyribonucleic acid (DNA) equal to that synthesized in permissively infected HeLa cells. However, there was 6,000-fold less virion produced in CHO cells.
M, Longiaru, M S, Horwitz
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Sterilization of PAN/Gelatine Nanofibrous Mats for Cell Growth [PDF]
Tekstilec, 2019 Nanofibrous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts) have been shown to grow well on fine fibres.
Daria Wehlage +8 more
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CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering
International Journal of Molecular Sciences, 2023 The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and ...
Katja Glinšek +2 more
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Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
Frontiers in Bioengineering and Biotechnology, 2021 At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs.
Weifeng Li +4 more
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Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells [PDF]
Analytical Chemistry, 2018 The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO). The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects,
Ganglong Yang +7 more
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Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures [PDF]
, 2017 Vesivirus 2117 is an adventitious agent that in 2009, was identified as a contaminant of CHO cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme.
Bhella, David +8 more
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Data in Brief, 2015 This data article shows how the recombinant Chinese Hamster Ovary (CHO) cells are located in the interstices of the matrix fibers of a CellTank bioreactor after completion of a perfusion culture, supporting the article entitled “Very high cell density ...
Ye Zhang, Véronique Chotteau
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One-Step Enrichment of Intact Glycopeptides From Glycoengineered Chinese Hamster Ovary Cells
Frontiers in Chemistry, 2020 Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level.
Ganglong Yang +8 more
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