Results 51 to 60 of about 83,358 (225)

Assessment of the cytotoxicity of chlorhexidine by employing an in vitro mammalian test system

Journal of Dental Sciences, 2014
Background/purpose: Chlorhexidine (CHX), a chlorophenyl biguanide with broad antibacterial action, has been widely used in dentistry. The initial uses of CHX in dentistry were to wash operation site and to disinfect root canals. Recently, the addition of
Yi-Ching Li, Yu-Hsiang Kuan, Tzu-Hsin Lee, Fu-Mei Huang, Yu-Chao Chang   +4 more
doaj   +1 more source

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells [PDF]

, 2015
Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non ...
Andersen, Mikael Rørdam, Grav, Lise Marie, Hansen, Henning Gram, Kildegaard, Helene Faustrup, Kol, Stefan, Lund, Anne Mathilde, Lundqvist, Magnus, Min Lee, Gyun, Nilsson, Claes Nymand, Rockberg, Johan   +9 more
core   +1 more source

Autosomal Dominant Erythrocytosis Caused by Non‐Renal Erythropoietin (EPO) Due to EPO c.‐136 G>A Germline Mutation

American Journal of Hematology, EarlyView.
A novel erythropoietin (EPO) promoter mutation (c.‐136 G>A) causes autosomal dominant erythrocytosis via non‐renal expression of EPO. ABSTRACT We previously reported a five‐generation kindred with autosomal dominant erythrocytosis associated with a novel germline promoter variant in the erythropoietin (EPO) gene (EPO c.‐136 G>A).
Lucie Lanikova, Dusan Hrckulak, Veronika Zimolova, Felipe R. Lorenzo, Jihyun Song, Katerina Vecerkova, Olga Babosova, Linda Berkova, Vladimir Korinek, Steve Elliott, Josef T. Prchal   +10 more
wiley   +1 more source

Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons [version 1; peer review: 3 approved]

AMRC Open Research, 2020
Good’s buffers are commonly used for cell culture and, although developed to have minimal to no biological impact, they cause alterations in cellular processes such as autophagy and lysosomal enzyme activity. Using Chinese hamster ovary cells and induced
Nicholas D. Allen, Helen Waller-Evans, Emyr Lloyd-Evans, Rafael A. Badell-Grau, Sophie R. Cook, Kimberley M. Jones, Emily D. Kirkham, Jincy Winston, Brendan P. Kelly   +8 more
doaj   +1 more source

Dynamic Binder Exchange Improves Protein Labeling Efficiency in DNA‐PAINT up to 15‐Fold

Angewandte Chemie International Edition, EarlyView.
Dynamic Binder Exchange (DyBE) leverages transient binder–target interactions to enhance labeling efficiency by up to 15‐fold in super‐resolution microscopy. DyBE maps nanoscale receptor tyrosine kinase (RTK) assemblies, including HER2 homodimers and EGFR–HER2 heterodimers, advancing single‐protein spatial proteomics.
Clemens Steinek, Isabelle Pachmayr, Sebastian Strauss, Monique Honsa, Jisoo Kwon, Ralf Jungmann   +5 more
wiley   +1 more source

What can mathematical modelling say about CHO metabolism and protein glycosylation?

Computational and Structural Biotechnology Journal, 2017
Chinese hamster ovary cells have been in the spotlight for process optimization in recent years, due to being the major, long established cell factory for the production of recombinant proteins.
Sarah N. Galleguillos, David Ruckerbauer, Matthias P. Gerstl, Nicole Borth, Michael Hanscho, Jürgen Zanghellini   +5 more
doaj   +1 more source

Comparison of protein precipitation methods for sample preparation prior to proteomic analysis of Chinese hamster ovary cell homogenates

Electronic Journal of Biotechnology, 2020
Background: Chinese hamster ovary (CHO) cells are the workhorse for obtaining recombinant proteins. Proteomic studies of these cells intend to understand cell biology and obtain more productive and robust cell lines for therapeutic protein production in ...
Saumel Pérez-Rodriguez, Octavio T. Ramírez, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruz   +3 more
doaj   +1 more source

In vitro approach to the study of chronic exposure to low doses of x-rays [PDF]

, 2011
El presente trabajo fue realizado con el fin de estudiar el efecto de dosis bajas y repetidas de radiación sobre dos líneas celulares de la misma especie. Se desarrolló un modelo in vitro para evitar la influencia de los factores de confusión que afectan
Peral Garcia, Pilar, Ponzinibbio, Maria Virginia, Seoane, Analia Isabel   +2 more
core   +1 more source

Three-dimensional tissue scaffolds from interbonded poly(e-caprolactone) fibrous matrices with controlled porosity

, 2011
In this article, we report on the preparation and cell culture performance of a novel fibrous matrix that has an interbonded fiber architecture, excellent pore interconnectivity, and controlled pore size and porosity.
Kirkland, Mark, Lin, Tong, Sutti, Alessandra, Tang, Yanwei, Wang, Hongxia, Wang, Xungai, Wong, Cynthia   +6 more
core   +1 more source

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source