Results 81 to 90 of about 928,240 (334)
Population size estimation for quality control of ChIP-Seq datasets.
PLoS ONE, 2019 Chromatin immunoprecipitation followed by sequencing, i.e. ChIP-Seq, is a widely used experimental technology for the identification of functional protein-DNA interactions.
Semyon K Kolmykov +5 more
doaj +1 more source
Single-cell ChIP-seq reveals cell subpopulations defined by chromatin state
Nature Biotechnology, 2015 Chromatin profiling provides a versatile means to investigate functional genomic elements and their regulation. However, current methods yield ensemble profiles that are insensitive to cell-to-cell variation.
A. Rotem +6 more
semanticscholar +1 more source
KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer
Molecular Oncology, EarlyView.Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan +16 more
wiley +1 more source
A high‐throughput ChIP‐Seq for large‐scale chromatin studies
Molecular Systems Biology, 2015 We present a modified approach of chromatin immuno‐precipitation followed by sequencing (ChIP‐Seq), which relies on the direct ligation of molecular barcodes to chromatin fragments, thereby permitting experimental scale‐up. With Bar‐ChIP now enabling the
Christophe D Chabbert +6 more
doaj +1 more source
Quantifying the impact of inter-site heterogeneity on the distribution of ChIP-seq data
Frontiers in Genetics, 2014 Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) is a valuable tool for epigenetic studies. Analysis of the data arising from ChIP-seq experiments often requires implicit or explicit statistical modelling of the read counts.
Jonathan eCairns +3 more
doaj +1 more source
Transcriptionally active HERV-H retrotransposons demarcate topologically associating domains in human pluripotent stem cells. [PDF]
, 2019 Chromatin architecture has been implicated in cell type-specific gene regulatory programs, yet how chromatin remodels during development remains to be fully elucidated.
Amaral, Maria Luisa +21 more
core +2 more sources
Molecular Oncology, EarlyView.CIN85 is highly expressed in osteosarcoma, particularly in metastatic lesions. Its overexpression increases cell migration and Matrigel invasion, while silencing CIN85 suppresses these behaviors. Transcriptome analysis shows that CIN85 regulates MMP2, COL3A1, and Akt/mTOR signaling. Targeting these pathways reverses CIN85‐induced motility, highlighting
Iryna Horak +10 more
wiley +1 more source
Unveiling combinatorial regulation through the combination of ChIP information and in silico cis-regulatory module detection [PDF]
, 2012 Computationally retrieving biologically relevant cis-regulatory modules (CRMs) is not straightforward. Because of the large number of candidates and the imperfection of the screening methods, many spurious CRMs are detected that are as high scoring as ...
Fierro, Ana Carolina +5 more
core +2 more sources
FEBS Open Bio, EarlyView.ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand +2 more
wiley +1 more source
Measuring reproducibility of high-throughput experiments
, 2011 Reproducibility is essential to reliable scientific discovery in high-throughput experiments. In this work we propose a unified approach to measure the reproducibility of findings identified from replicate experiments and identify putative discoveries ...
Bickel, Peter J. +3 more
core +1 more source