Results 1 to 10 of about 663,360 (333)

Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase [PDF]

Brazilian Journal of Medical and Biological Research, 2001
We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells.
C.R.C. Franco, H.A.O. Rocha, E.S. Trindade, I.A.N. Santos, E.L. Leite, S.S. Veiga, H.B. Nader, C.P. Dietrich   +7 more
doaj   +3 more sources

Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines

Electronic Journal of Biotechnology, 2019
Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines.
Jing Li, Suzhen Wei, Chunlai Cao, Kangyue Chen, Hua He, Guoquan Gao   +5 more
doaj   +3 more sources

Bradykinin release avoids high molecular weight kininogen endocytosis. [PDF]

PLoS ONE, 2015
Human H-kininogen (120 kDa) plays a role in many pathophysiological processes and interacts with the cell surface through protein receptors and proteoglycans, which mediate H-kininogen endocytosis.
Igor Z Damasceno, Katia R B Melo, Fabio D Nascimento, Daianne S P Souza, Mariana S Araujo, Sinval E G Souza, Misako U Sampaio, Helena B Nader, Ivarne L S Tersariol, Guacyara Motta   +9 more
doaj   +5 more sources

Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells [PDF]

Scientific Reports
To meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu, Yan-Fang Wang, Jing-Jia Yuan, Chun-Liu Mi, Shao-Lei Geng, Xiao-yin Wang, Tian-Yun Wang   +6 more
doaj   +2 more sources

CHOGlycoNET: Comprehensive glycosylation reaction network for CHO cells. [PDF]

Metab Eng, 2023
Chinese hamster ovary (CHO) cells are extensively used for the production of glycoprotein therapeutics proteins, for which N-linked glycans are a critical quality attribute due to their influence on activity and immunogenicity. Manipulation of protein glycosylation is commonly achieved through cell or process engineering, which are often guided by ...
Kotidis P, Donini R, Arnsdorf J, Hansen AH, Voldborg BGR, Chiang AWT, Haslam SM, Betenbaugh M, Jimenez Del Val I, Lewis NE, Krambeck F, Kontoravdi C.   +11 more
europepmc   +7 more sources

An effective method to enhance transient gene expression by transfecting high-density CHO cells. [PDF]

AMB Express
Fu YS, Han XL, Li WD, Shan LL, Geng SL, Mi CL, Dong WH, Wang TY, Wang XY, Wang X, Wang T.   +10 more
europepmc   +3 more sources

Construction of a novel kinetic model for the production process of a CVA6 VLP vaccine in CHO cells. [PDF]

Cytotechnology, 2023
Xing Z, Nguyen TB, Kanai-Bai G, Yamano-Adachi N, Omasa T.   +4 more
europepmc   +3 more sources

An Easy And Fast Method For Production Of Chinese Hamster Ovary Cell Line Expressing And Secreting Human Recombinant Activin A [PDF]

Cell Journal, 2020
Objective Growth factors are key elements of embryonic stem cell (ESC) research. Cell line development in eukaryotes is a time-consuming procedure which usually takes 12-18 months. Here, we report an easy and fast method with which production of Chinese
Hassan Rassouli, Ali Sayadmanesh, Siamak Rezaeiani, Zahra Ghezelayagh, Mohammad Reza Gharaati, Tahamtani Yaser   +5 more
doaj   +1 more source

Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

Life, 2022
Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease.
Chisato Watanabe, Hirotoshi Shibuya, Yusuke Ichiyama, Eiichi Okamura, Setsuko Tsukiyama-Fujii, Tomoyuki Tsukiyama, Shoma Matsumoto, Jun Matsushita, Takuya Azami, Yoshiaki Kubota, Masahito Ohji, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno, Masaru Tamura, Ken-ichi Mizutani, Masatsugu Ema   +16 more
doaj   +1 more source

Production of Monoclonal Antibody Anti-NS1 Induced by DENV3 Indonesian Clinical Isolate to Detect Native NS1 Antigen of all DENV Serotypes [PDF]

Journal of Clinical and Diagnostic Research, 2018
Introduction: Dengue Virus (DENV) infection is a mosquito-borne disease, the most severe manifestation of the infection could lead to a lethal Dengue Haemorrhagic Fever (DHF) and/or Dengue Shock Syndrome (DSS).
Tika Widayanti, Beti Ernawati Dewi, Sabar Pambudi   +2 more
doaj   +1 more source