Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase [PDF]
Brazilian Journal of Medical and Biological Research, 2001 We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells.
C.R.C. Franco +7 more
doaj +3 more sources
Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells [PDF]
Scientific ReportsTo meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu +6 more
doaj +2 more sources
Bradykinin release avoids high molecular weight kininogen endocytosis. [PDF]
PLoS ONE, 2015 Human H-kininogen (120 kDa) plays a role in many pathophysiological processes and interacts with the cell surface through protein receptors and proteoglycans, which mediate H-kininogen endocytosis.
Igor Z Damasceno +9 more
doaj +5 more sources
Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines
Electronic Journal of Biotechnology, 2019 Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines.
Jing Li +5 more
doaj +4 more sources
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins [PDF]
Brazilian Journal of Medical and Biological Research, 2001 Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions.
H.A.O. Rocha +7 more
doaj +3 more sources
Glutamine synthetase (GS) knockout (KO) using CRISPR/Cpf1 diversely enhances selection efficiency of CHO cells expressing therapeutic antibodies [PDF]
Scientific Reports, 2023 The glutamine synthetase (GS)-based Chinese hamster ovary (CHO) selection system is an attractive approach to efficiently identify suitable clones in the cell line generation process for biologics manufacture, for which GS-knockout (GS-KO) CHO cell lines
Witsanu Srila +5 more
doaj +2 more sources
An Easy And Fast Method For Production Of Chinese Hamster Ovary Cell Line Expressing And Secreting Human Recombinant Activin A [PDF]
Cell Journal, 2020 Objective Growth factors are key elements of embryonic stem cell (ESC) research. Cell line development in eukaryotes is a time-consuming procedure which usually takes 12-18 months. Here, we report an easy and fast method with which production of Chinese
Hassan Rassouli +5 more
doaj +1 more source
Comparison of Human Factor IX Abundance and mRNA Expression Levels In Stable Insect and Mammalian Cells [PDF]
Journal of Cell and Molecular Research, 2017 In the production of recombinant proteins, the selection of an expression system is very important. Although CHO cells are used as mammalian expression system, the ability of insect Schneider line 2 (S2) cells as a new expression system for the high ...
Jafar Vatandoost, Shadi Tirdad
doaj +1 more source
Production of Monoclonal Antibody Anti-NS1 Induced by DENV3 Indonesian Clinical Isolate to Detect Native NS1 Antigen of all DENV Serotypes [PDF]
Journal of Clinical and Diagnostic Research, 2018 Introduction: Dengue Virus (DENV) infection is a mosquito-borne disease, the most severe manifestation of the infection could lead to a lethal Dengue Haemorrhagic Fever (DHF) and/or Dengue Shock Syndrome (DSS).
Tika Widayanti +2 more
doaj +1 more source
Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
Life, 2022 Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease.
Chisato Watanabe +16 more
doaj +1 more source