Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase [PDF]
Brazilian Journal of Medical and Biological Research, 2001 We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells.
C.R.C. Franco +7 more
doaj +3 more sources
Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines
Electronic Journal of Biotechnology, 2019 Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines.
Jing Li +5 more
doaj +3 more sources
Bradykinin release avoids high molecular weight kininogen endocytosis. [PDF]
PLoS ONE, 2015 Human H-kininogen (120 kDa) plays a role in many pathophysiological processes and interacts with the cell surface through protein receptors and proteoglycans, which mediate H-kininogen endocytosis.
Igor Z Damasceno +9 more
doaj +5 more sources
CHOGlycoNET: Comprehensive glycosylation reaction network for CHO cells. [PDF]
Metab Eng, 2023 Chinese hamster ovary (CHO) cells are extensively used for the production of glycoprotein therapeutics proteins, for which N-linked glycans are a critical quality attribute due to their influence on activity and immunogenicity. Manipulation of protein glycosylation is commonly achieved through cell or process engineering, which are often guided by ...
Kotidis P +11 more
europepmc +7 more sources
Epigenetic regulation of histone methyltransferase SUV39H1 on the expression of recombinant protein in CHO cells [PDF]
Applied Microbiology and BiotechnologyAbstract Histone methylation–mediated epigenetic modification significantly influences gene transcription and expression regulation. This study examined the effects of histone 3 lysine 9 trimethylation (H3K9me3) methyltransferase SUV39H1 and its specific
Lu-Lu Yang +9 more
doaj +2 more sources
Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells [PDF]
Scientific ReportsTo meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu +6 more
doaj +2 more sources
SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing [PDF]
AMB ExpressChinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges.
Jiang-Tao Lu +12 more
doaj +2 more sources
An Easy And Fast Method For Production Of Chinese Hamster Ovary Cell Line Expressing And Secreting Human Recombinant Activin A [PDF]
Cell Journal, 2020 Objective Growth factors are key elements of embryonic stem cell (ESC) research. Cell line development in eukaryotes is a time-consuming procedure which usually takes 12-18 months. Here, we report an easy and fast method with which production of Chinese
Hassan Rassouli +5 more
doaj +1 more source
Production of Monoclonal Antibody Anti-NS1 Induced by DENV3 Indonesian Clinical Isolate to Detect Native NS1 Antigen of all DENV Serotypes [PDF]
Journal of Clinical and Diagnostic Research, 2018 Introduction: Dengue Virus (DENV) infection is a mosquito-borne disease, the most severe manifestation of the infection could lead to a lethal Dengue Haemorrhagic Fever (DHF) and/or Dengue Shock Syndrome (DSS).
Tika Widayanti +2 more
doaj +1 more source
Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
Life, 2022 Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease.
Chisato Watanabe +16 more
doaj +1 more source