Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells [PDF]
Scientific ReportsTo meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu +6 more
doaj +2 more sources
Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines
Electronic Journal of Biotechnology, 2019 Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines.
Jing Li +5 more
doaj +4 more sources
An Easy And Fast Method For Production Of Chinese Hamster Ovary Cell Line Expressing And Secreting Human Recombinant Activin A [PDF]
Cell Journal, 2020 Objective Growth factors are key elements of embryonic stem cell (ESC) research. Cell line development in eukaryotes is a time-consuming procedure which usually takes 12-18 months. Here, we report an easy and fast method with which production of Chinese
Hassan Rassouli +5 more
doaj +1 more source
Comparison of Human Factor IX Abundance and mRNA Expression Levels In Stable Insect and Mammalian Cells [PDF]
Journal of Cell and Molecular Research, 2017 In the production of recombinant proteins, the selection of an expression system is very important. Although CHO cells are used as mammalian expression system, the ability of insect Schneider line 2 (S2) cells as a new expression system for the high ...
Jafar Vatandoost, Shadi Tirdad
doaj +1 more source
Production of Monoclonal Antibody Anti-NS1 Induced by DENV3 Indonesian Clinical Isolate to Detect Native NS1 Antigen of all DENV Serotypes [PDF]
Journal of Clinical and Diagnostic Research, 2018 Introduction: Dengue Virus (DENV) infection is a mosquito-borne disease, the most severe manifestation of the infection could lead to a lethal Dengue Haemorrhagic Fever (DHF) and/or Dengue Shock Syndrome (DSS).
Tika Widayanti +2 more
doaj +1 more source
Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability
Biological Research, 2020 Background Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell in the commercial-scale production of biopharmaceutical proteins. Modification of genes involved in apoptosis may improve the productivity of CHO cells. Executive
Fatemeh Safari +5 more
doaj +1 more source
Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
Life, 2022 Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease.
Chisato Watanabe +16 more
doaj +1 more source
Towards Tumor Targeting via Invasive Assay Using Magnetospirillum magneticum
Frontiers in Microbiology, 2021 Magnetospirillum magneticum (AMB-1) are a species of magnetotactic bacteria (MTB) that are capable of orienting along the earth’s magnetic field lines through their organelles called magnetosomes.
Marvin Xavierselvan +4 more
doaj +1 more source
Separation of CHO cells using hydrocyclones [PDF]
Cytotechnology, 2007 Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design.
Leda R. Castilho +2 more
openaire +3 more sources
Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells
Frontiers in Cellular and Infection Microbiology, 2021 In a Plasmodium vivax infection, it was shown a proportionally increased on gametocyte distribution within the bone marrow aspirant, suggesting a role of this organ as a reservoir for this parasite stage.
Luis Carlos Salazar Alvarez +17 more
doaj +1 more source