Results 11 to 20 of about 188,771 (295)

Next-generation sequencing of the CHO cell transcriptome [PDF]

BMC Proceedings, 2011
Since 1957 Chinese hamster ovary (CHO) cells are used for in vitro cultivation as they require assimilable low sustenance [1]. Today, CHO cell lines represent the most commonly used mammalian expression system for the production of therapeutic proteins and are considered as the mammalian equivalent of E. coli in research and biotechnology [2].
Becker, Jennifer, Timmermann, Christina, Jakobi, Tobias, Rupp, Oliver, Szczepanowski, Rafael, Hackl, Matthias, Goesmann, Alexander, Tauch, Andreas, Borth, Nicole, Grillari, Johannes, Pühler, Alfred, Noll, Thomas, Brinkrolf, Karina, Hauser, Hansjörg   +13 more
openaire   +6 more sources

CD300LG improves the cytotoxic activity of CIK

Central European Journal of Immunology, 2017
To investigate the effect of CD300LG-γ induction on the cytotoxic activity of CIK. Eukaryotic expression plasmid hCD300LG-γ/pEGFP-C3, which can express human CD300LG-γ, was constructed and transfected into CHO cells by lipofectamine.
Qiuyue Wang, Yuqing Liu, Ying Chen, Ke Wang, Wensheng Xie, Dapeng Wei, Lijuan Hu   +6 more
doaj   +1 more source

USING OF UNIVERSAL PLASMID CONSTRUCTIONS FOR DESIGN OF RECOMBINANT ANTIBODIES WITH DEFINED SPECIFICITY IN EUKARYOTIC CELLS

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. In this study we aimed to develop the methodology to change the antigen specificity of chimeric antibodies by replacing the variable region genes in the previously designed universal plasmid constructions pLK DT-17 and pHG DT-17 encoding the DT-17 ...
T. G. Samartseva, A. S. Oksanich, N. F. Gavrilova, I. V. Yakovleva, V. V. Sviridov, V. V. Zverev   +5 more
doaj   +1 more source

Glycoproteomic Characterization of FUT8 Knock-Out CHO Cells Reveals Roles of FUT8 in the Glycosylation

Frontiers in Chemistry, 2021
The α1,6-fucosyltransferase (encoded by FUT8 gene) is the key enzyme transferring fucose to the innermost GlcNAc residue on an N-glycan through an α-1,6 linkage in the mammalian cells.
Ganglong Yang, Qiong Wang, Lijun Chen, Michael J. Betenbaugh, Hui Zhang, Hui Zhang   +5 more
doaj   +1 more source

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu, Hui-Ning Liu, Wei-Hua Dong, Wei-Hua Dong, Yan Lin, Zhao-Hui Zhang, Tian-Yun Wang, Tian-Yun Wang   +7 more
doaj   +1 more source

High Throughput miRNA Screening Identifies miR-574-3p Hyperproductive Effect in CHO Cells

Biomolecules, 2021
CHO is the cell line of choice for the manufacturing of many complex biotherapeutics. The constant upgrading of cell productivity is needed to meet the growing demand for these life-saving drugs.
Živa Švab, Luca Braga, Corrado Guarnaccia, Ivan Labik, Jeremias Herzog, Marco Baralle, Mauro Giacca, Nataša Skoko   +7 more
doaj   +1 more source

S-Sulfocysteine – Investigation of cellular uptake in CHO cells

Journal of Biotechnology, 2021
For the generation of therapeutic proteins in cell culture, high producing clones are used. These clones have a high demand in amino acids to support cell growth and productivity. l-cysteine (Cys) is critical in highly concentrated feeds due to low stability of Cys and low solubility of the oxidation product cystine at neutral pH. S-sulfocysteine (SSC)
Aline Zimmer, Harald Kolmar, Martina Zimmermann, Martina Zimmermann   +3 more
openaire   +4 more sources

Production and purification of TGFb-1 in CHO-Cells [PDF]

BMC Proceedings, 2011
The development of chemically well defined media is a demanding task in order to create the optimal conditions for an in vitro stem cell (SC) proliferation and differentiation system. Signals that govern SC differentiation into multiple mature cell types are provided by growth factors.
Sabrina Baganz, Estabraq Abdulkerim, Cornelia Kasper, Axel Schambach, Thomas Scheper   +4 more
openaire   +4 more sources

Engineering CHO cell metabolism for growth in galactose [PDF]

BMC Proceedings, 2011
Background Chinese hamster ovary (CHO) cells are one of the main hosts for industrial production of therapeutic proteins, owing to well-characterized technologies for gene transfection, amplification, and selection of high-producer clones. This has motivated the search for different strategies for the improvement of their specific productivity being ...
Ziomara P. Gerdtzen, Camila A Wilkens, Natalia E Jiménez   +2 more
openaire   +4 more sources

Microparticle-mediated transfer of the viral receptors CAR and CD46, and the CFTR channel in a CHO cell model confers new functions to target cells.

PLoS ONE, 2012
Cell microparticles (MPs) released in the extracellular milieu can embark plasma membrane and intracellular components which are specific of their cellular origin, and transfer them to target cells.
Gaëlle Gonzalez, Cyrielle Vituret, Attilio Di Pietro, Marc Chanson, Pierre Boulanger, Saw-See Hong   +5 more
doaj   +1 more source