Results 21 to 30 of about 663,360 (333)

High-Level Expression of Various Apolipoprotein (a) Isoforms by "Transferrinfection". The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein [PDF]

, 1994
Characterization of the assembly of lipoprotein(a) [Lp(a)] is of fundamental importance to understanding the biosynthesis and metabolism of this atherogenic lipoprotein. Since no established cell lines exist that express Lp(a) or apolipoprotein(a) [apo(a)
Azrolan N., Boerwinkle E., Brunner C., Chiesa G., Chung B. Y., Cotten M., Deutscher S. L., Deutscher S. L., Dieplinger H., Edelberg J. M., Green S., Griffiths G., Guevara J., Jr., Huby T., Ichinose A., Kamboh M. I., Koschinsky M. L., Koschinsky M. L., Koschinsky M. L., Kostner G. M., Kraft H- G., Kraft H. G., Krempler F., Krempler F., Lackner C., Linton M. F., Malgaretti N., McGregor G. R., McLean J. W., Menzel H. J., Phillips M. L., Rader D. J., Sambrook J., Sandholzer C., Scanu A. M., Selden R. F., Sommer A., Stanley P., Trieu V. N., Utermann G., Utermann G., Utermann G., Wagner E., White A. L., Zatloukal K., Zechner R„   +45 more
core   +1 more source

CD300LG improves the cytotoxic activity of CIK

Central European Journal of Immunology, 2017
To investigate the effect of CD300LG-γ induction on the cytotoxic activity of CIK. Eukaryotic expression plasmid hCD300LG-γ/pEGFP-C3, which can express human CD300LG-γ, was constructed and transfected into CHO cells by lipofectamine.
Qiuyue Wang, Yuqing Liu, Ying Chen, Ke Wang, Wensheng Xie, Dapeng Wei, Lijuan Hu   +6 more
doaj   +1 more source

Glycoproteomic Characterization of FUT8 Knock-Out CHO Cells Reveals Roles of FUT8 in the Glycosylation

Frontiers in Chemistry, 2021
The α1,6-fucosyltransferase (encoded by FUT8 gene) is the key enzyme transferring fucose to the innermost GlcNAc residue on an N-glycan through an α-1,6 linkage in the mammalian cells.
Ganglong Yang, Qiong Wang, Lijun Chen, Michael J. Betenbaugh, Hui Zhang, Hui Zhang   +5 more
doaj   +1 more source

Histamine H1 Receptor Down-Regulation Mediated by M3 Muscarinic Acetylcholine Receptor Subtype

Journal of Pharmacological Sciences, 2004
Heterologous down-regulation of histamine H1 receptor (H1R) mediated by muscarinic acetylcholine receptor subtype was investigated using five kinds of Chinese hamster ovary (CHO) cells stably co-expressing the human H1R and one of the five (M1 –M5 ...
Katsuhiro Miyoshi, Nozomi Kawakami, Yousuke Wakayama, Norimasa Izumi, Shuhei Horio, Hiroyuki Fukui   +5 more
doaj   +1 more source

Cell Propagation of Cholera Toxin CTA ADP-Ribosylating Factor by Exosome Mediated Transfer [PDF]

, 2018
In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population.
Boussadia, Zaira, Carollo, Maria, Fabbri, Alessia, Falchi, Mario, Fecchi, Katia, Fiani, Maria Luisa, Gallina, Angelo, Palermo, Angela, Parisi, Sofia, Pasquini, Luca, Sargiacomo, Massimo, Zanetti, Cristiana   +11 more
core   +1 more source

Truncated human endothelin receptor A produced by alternative splicing and its expression in melanoma [PDF]

, 1998
In this study, reverse transcriptase polymerase chain reaction was used to amplify human endothelin receptor A (ETA) and ETB receptor mRNA. A truncated ETA receptor transcript with exons 3 and 4 skipped was found.
Berry, PA, Burchill, SA, Carter, ND, Jeffery, S, Kaski, JC, Zhang, YF   +5 more
core   +1 more source

Optimization of human interferon beta protein expression in Chinese hamster ovary cells [PDF]

Journal of Shahrekord University of Medical Sciences, 2021
Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ...
Nasrin Xodadadi, Alireza Saeidinia, Mehdi Zeinoddini, Rasoul Khalilzadeh   +3 more
doaj   +1 more source

The GalNAc-type O-Glycoproteome of CHO Cells Characterized by the SimpleCell Strategy [PDF]

, 2014
The Chinese hamster ovary cell (CHO) is the major host cell factory for recombinant production of biological therapeutics primarily because of its “human-like” glycosylation features.
Alder Schulz, Morten, Clausen, Henrik, Gram Schjoldager, Katrine Ter-Borch, Halim, Adnan, Jitendra Joshi, Hiren, Kayser, Kevin J., Levery, Steven B., Narimatsu, Yoshiki, Paul Bennett, Eric, Sealover, Natalie R., Steentoft, Catharina, Vakhrushev, Sergey Y., Zhang, Yang   +12 more
core   +1 more source

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu, Hui-Ning Liu, Wei-Hua Dong, Wei-Hua Dong, Yan Lin, Zhao-Hui Zhang, Tian-Yun Wang, Tian-Yun Wang   +7 more
doaj   +1 more source

Correction of the neuropathogenic human apolipoprotein E4 (APOE4) gene to APOE3 in vitro using synthetic RNA/DNA oligonucleotides (chimeraplasts) [PDF]

, 2005
Apolipoprotein E (apoE) is a multifunctional circulating 34-kDa protein, whose gene encodes single-nucleotide polymorphisms linked to several neurodegenerative diseases.
Dickson, JG, Owen, JS, Simons, JP, Tagalakis, AD   +3 more
core   +1 more source