Results 21 to 30 of about 188,771 (295)
CHO cell lines generated by PiggyBac transposition [PDF]
BMC Proceedings, 2011 A major bottleneck in the manufacture of recombinant therapeutic proteins is the time and effort needed for the generation of stable, high-producing mammalian cell lines. Conventional gene transfer methods for stable cell line generation rely on random transgene integration, resulting in unpredictable and highly variable levels of expression of the ...
Lucia Baldi +5 more
openaire +4 more sources
Brazilian Journal of Medical and Biological Research, 2001 We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells.
C.R.C. Franco +7 more
doaj +1 more source
Modulation of the Plasma Kallikrein-Kinin System Proteins Performed by Heparan Sulfate Proteoglycans
Frontiers in Physiology, 2017 Human plasma kallikrein-kinin system proteins are related to inflammation through bradykinin. In the proximity of its target cells, high molecular weight kininogen (H-kininogen) is the substrate of plasma kallikrein, which releases bradykinin from H ...
Guacyara Motta, Ivarne L. S. Tersariol
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Awakening dormant glycosyltransferases in CHO cells with CRISPRa [PDF]
Biotechnology and Bioengineering, 2019 AbstractChinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.
Lasse Ebdrup Pedersen +12 more
openaire +6 more sources
Hyperosmolality in CHO cell culture: effects on the proteome
Applied Microbiology and Biotechnology, 2022 AbstractChinese hamster ovary (CHO) cells are the most commonly used host cell lines for therapeutic protein production. Exposure of these cells to highly concentrated feed solution during fed-batch cultivation can lead to a non-physiological increase in osmolality (> 300 mOsm/kg) that affects cell physiology, morphology, and proteome.
Nadiya Romanova +3 more
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Biological Research, 2022 Background Chinese hamster ovary cell line has been used routinely as a bioproduction factory of numerous biopharmaceuticals. So far, various engineering strategies have been recruited to improve the production efficiency of this cell line such as ...
Fatemeh Safari, Bahman Akbari
doaj +1 more source
Bradykinin release avoids high molecular weight kininogen endocytosis. [PDF]
PLoS ONE, 2015 Human H-kininogen (120 kDa) plays a role in many pathophysiological processes and interacts with the cell surface through protein receptors and proteoglycans, which mediate H-kininogen endocytosis.
Igor Z Damasceno +9 more
doaj +1 more source
Investigation of the bystander effect in CHO-K1 cells [PDF]
Reports of Practical Oncology & Radiotherapy, 2014 Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1-4 Gy of high LET 12C ions and X-rays.The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron ...
Dariusz Banaś +13 more
openaire +3 more sources
Production of α2,6-sialylated IgG1 in CHO cells [PDF]
mAbs, 2015 The presence of α2,6-sialic acids on the Fc N-glycan provides anti-inflammatory properties to the IgGs through a mechanism that remains unclear. Fc-sialylated IgGs are rare in humans as well as in industrial host cell lines such as Chinese hamster ovary (CHO) cells.
Raymond, Céline +5 more
openaire +3 more sources
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth ...
Zheng-Mei Li +7 more
doaj +1 more source