Results 31 to 40 of about 10,981,319 (237)
CHO cell lines generated by PiggyBac transposition [PDF]
BMC Proceedings, 2011 A major bottleneck in the manufacture of recombinant therapeutic proteins is the time and effort needed for the generation of stable, high-producing mammalian cell lines. Conventional gene transfer methods for stable cell line generation rely on random transgene integration, resulting in unpredictable and highly variable levels of expression of the ...
Lucia Baldi +5 more
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Biotechnology and Bioengineering, 2016 The most striking characteristic of CHO cells is their adaptability, which enables efficient production of proteins as well as growth under a variety of culture conditions, but also results in genomic and phenotypic instability.
J. Feichtinger +18 more
semanticscholar +1 more source
Brazilian Journal of Medical and Biological Research, 2001 We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells.
C.R.C. Franco +7 more
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Biotechnology Journal, 2018 Despite great efforts to control and modify gene expression of Chinese Hamster Ovary (CHO) cells by conventional genetic engineering approaches, i.e. overexpression or knockdown/-out, subclonal variation, induced unknown regulatory effects as well as ...
N. Marx +6 more
semanticscholar +1 more source
Modulation of the Plasma Kallikrein-Kinin System Proteins Performed by Heparan Sulfate Proteoglycans
Frontiers in Physiology, 2017 Human plasma kallikrein-kinin system proteins are related to inflammation through bradykinin. In the proximity of its target cells, high molecular weight kininogen (H-kininogen) is the substrate of plasma kallikrein, which releases bradykinin from H ...
Guacyara Motta, Ivarne L. S. Tersariol
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Biological Research, 2022 Background Chinese hamster ovary cell line has been used routinely as a bioproduction factory of numerous biopharmaceuticals. So far, various engineering strategies have been recruited to improve the production efficiency of this cell line such as ...
Fatemeh Safari, Bahman Akbari
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Hyperosmolality in CHO cell culture: effects on the proteome
Applied Microbiology and Biotechnology, 2022 AbstractChinese hamster ovary (CHO) cells are the most commonly used host cell lines for therapeutic protein production. Exposure of these cells to highly concentrated feed solution during fed-batch cultivation can lead to a non-physiological increase in osmolality (> 300 mOsm/kg) that affects cell physiology, morphology, and proteome.
Nadiya Romanova +3 more
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bioRxiv, 2019 Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies.
Hamideh Fouladiha +5 more
semanticscholar +1 more source
Bradykinin release avoids high molecular weight kininogen endocytosis. [PDF]
PLoS ONE, 2015 Human H-kininogen (120 kDa) plays a role in many pathophysiological processes and interacts with the cell surface through protein receptors and proteoglycans, which mediate H-kininogen endocytosis.
Igor Z Damasceno +9 more
doaj +1 more source
Investigation of the bystander effect in CHO-K1 cells [PDF]
Reports of Practical Oncology & Radiotherapy, 2014 Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1-4 Gy of high LET 12C ions and X-rays.The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron ...
Dariusz Banaś +13 more
openaire +3 more sources