Results 41 to 50 of about 11,449,307 (399)
PLoS ONE, 2012 Cell microparticles (MPs) released in the extracellular milieu can embark plasma membrane and intracellular components which are specific of their cellular origin, and transfer them to target cells.
Gaëlle Gonzalez +5 more
doaj +1 more source
Truncated human endothelin receptor A produced by alternative splicing and its expression in melanoma [PDF]
, 1998 In this study, reverse transcriptase polymerase chain reaction was used to amplify human endothelin receptor A (ETA) and ETB receptor mRNA. A truncated ETA receptor transcript with exons 3 and 4 skipped was found.
Berry, PA +5 more
core +1 more source
Engineering CHO cell metabolism for growth in galactose [PDF]
BMC Proceedings, 2011 Background Chinese hamster ovary (CHO) cells are one of the main hosts for industrial production of therapeutic proteins, owing to well-characterized technologies for gene transfection, amplification, and selection of high-producer clones. This has motivated the search for different strategies for the improvement of their specific productivity being ...
Ziomara P. Gerdtzen +2 more
openaire +4 more sources
Biotechnology and Bioengineering, 2016 The most striking characteristic of CHO cells is their adaptability, which enables efficient production of proteins as well as growth under a variety of culture conditions, but also results in genomic and phenotypic instability.
J. Feichtinger +18 more
semanticscholar +1 more source
Biotechnology Journal, 2018 Despite great efforts to control and modify gene expression of Chinese Hamster Ovary (CHO) cells by conventional genetic engineering approaches, i.e. overexpression or knockdown/-out, subclonal variation, induced unknown regulatory effects as well as ...
N. Marx +6 more
semanticscholar +1 more source
CHO cell lines generated by PiggyBac transposition [PDF]
BMC Proceedings, 2011 A major bottleneck in the manufacture of recombinant therapeutic proteins is the time and effort needed for the generation of stable, high-producing mammalian cell lines. Conventional gene transfer methods for stable cell line generation rely on random transgene integration, resulting in unpredictable and highly variable levels of expression of the ...
Lucia Baldi +5 more
openaire +4 more sources
bioRxiv, 2019 Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies.
Hamideh Fouladiha +5 more
semanticscholar +1 more source
Modulation of the Plasma Kallikrein-Kinin System Proteins Performed by Heparan Sulfate Proteoglycans
Frontiers in Physiology, 2017 Human plasma kallikrein-kinin system proteins are related to inflammation through bradykinin. In the proximity of its target cells, high molecular weight kininogen (H-kininogen) is the substrate of plasma kallikrein, which releases bradykinin from H ...
Guacyara Motta, Ivarne L. S. Tersariol
doaj +1 more source
Hyperosmolality in CHO cell culture: effects on the proteome
Applied Microbiology and Biotechnology, 2022 AbstractChinese hamster ovary (CHO) cells are the most commonly used host cell lines for therapeutic protein production. Exposure of these cells to highly concentrated feed solution during fed-batch cultivation can lead to a non-physiological increase in osmolality (> 300 mOsm/kg) that affects cell physiology, morphology, and proteome.
Nadiya Romanova +3 more
openaire +2 more sources
Optimization of human interferon beta protein expression in Chinese hamster ovary cells [PDF]
Journal of Shahrekord University of Medical Sciences, 2021 Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ...
Nasrin Xodadadi +3 more
doaj +1 more source