Results 41 to 50 of about 644,201 (301)

Bioreactor cultivation of CHO DP-12 cells under sodium butyrate treatment – comparative transcriptome analysis with CHO cDNA microarrays [PDF]

, 2011
Klausing S, Krämer O, Noll T. Bioreactor cultivation of CHO DP-12 cells under sodium butyrate treatment – comparative transcriptome analysis with CHO cDNA microarrays. BMC Proceedings. 2011;5(Suppl 8): P98
Sandra Klausing, Oliver Krämer, Thomas Noll   +2 more
core   +2 more sources

GRP78 protects CHO cells from ribosylation

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2018
d-Ribose (Rib), a reactive glycation compound that exists in organisms, abnormally increases in the urine of diabetic patients and can yield large amounts of advanced glycation end products (AGEs), leading to cell dysfunction. However, whether cellular proteins are sensitive to this type of glycation is unknown.
Wu, Beibei, Yu, Lexiang, Hu, Pingdong, Lu, Yang, Li, Juan, Wei, Yan, He, Rongqiao, Y. Wei, R. He   +8 more
openaire   +3 more sources

Amplification Using CHO Cell Expression Vectors [PDF]

Current Protocols in Molecular Biology, 2002
AbstractThe ability to select for integration of plasmid DNA into the host chromosome allows the generation of stably transfected cell lines. With transfection of a selectable marker linked to a nonselectable target gene (or by cotransfection of the two unlinked genes), high‐level expression of the desired gene is obtained by selecting for ...
Kingston, Robert E., Kaufman, Randal J., Bebbington, C.R., Rolfe, M.R.   +3 more
openaire   +3 more sources

Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells [PDF]

, 2011
Introduction The inflammatory enzyme indoleamine 2, 3-dioxygenase (IDO) participates in immune tolerance and promotes immune escape of IDO+ tumors. A recent hypothesis suggested that IDO may contribute to the differentiation of new T regulatory cells ...
Liu Juntian, Yu Wenwen, Wei Feng, Yang Lili, Li Hui, Yu Jinpu, Sun Jingyan, Ren Xiubao   +7 more
core   +2 more sources

Interaction of Clostridium perfringens Epsilon Toxin with the Plasma Membrane: The Role of Amino Acids Y42, Y43 and H162

Toxins, 2022
Clostridium perfringens epsilon toxin (Etx) is a pore forming toxin that causes enterotoxaemia in ruminants and may be a cause of multiple sclerosis in humans. To date, most in vitro studies of Etx have used the Madin-Darby canine kidney (MDCK) cell line.
Skye Marshall, Beth McGill, Helen Morcrette, C. Peter Winlove, Catalin Chimerel, Peter G. Petrov, Monika Bokori-Brown   +6 more
doaj   +1 more source

The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells [PDF]

, 2005
The polarized distribution of Na+,K+-ATPase plays a paramount physiological role, because either directly or through coupling with co- and countertransporters, it is responsible for the net movement of, for example, glucose, amino acids, Ca2+, K+, Cl ...
Balda, MS, Cereijido, M, Contreras, RG, Lazaro, A, Matter, K, Moreno, J, Roldan, ML, Shoshani, L   +7 more
core  

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Serine one-carbon catabolism with formate overflow [PDF]

, 2016
Serine catabolism to glycine and a one-carbon unit has been linked to the anabolic requirements of proliferating mammalian cells. However, genome-scale modeling predicts a catabolic role with one-carbon release as formate. We experimentally prove that in
Athineos, Dimitris, Blyth, Karen, Gottlieb, Eyal, Kamphorst, Jurre J., Labuschagne, Christiaan Fred, Mackay, Gillian M., Maddocks, Oliver, Meiser, Johannes, Tumanov, Sergey, Van Den Broek, Niels, Vazquez, Alexei, Vousden, Karen   +11 more
core   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Development of technology for culturing a cell line producing a single-domain antibody fused with the Fc fragment of human IgG1

Тонкие химические технологии
Objectives. To develop an effective technology for the cultivation of Chinese hamster ovary (CHO) cells stably producing GamP2C5 antibody which is a component I of the GamCoviMab candidate drug for emergency prevention and therapy of  infection caused by
D. S. Polyansky, E. I. Ryabova, A. A. Derkaev, N. S. Starkov, I. S. Kashapova, D. V. Shcheblyakov, A. P. Karpov, I. B. Esmagambetov   +7 more
doaj   +1 more source