Results 41 to 50 of about 663,360 (333)

GRP78 protects CHO cells from ribosylation

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2018
d-Ribose (Rib), a reactive glycation compound that exists in organisms, abnormally increases in the urine of diabetic patients and can yield large amounts of advanced glycation end products (AGEs), leading to cell dysfunction. However, whether cellular proteins are sensitive to this type of glycation is unknown.
Wu, Beibei, Yu, Lexiang, Hu, Pingdong, Lu, Yang, Li, Juan, Wei, Yan, He, Rongqiao, Y. Wei, R. He   +8 more
openaire   +3 more sources

Amplification Using CHO Cell Expression Vectors [PDF]

Current Protocols in Molecular Biology, 2002
AbstractThe ability to select for integration of plasmid DNA into the host chromosome allows the generation of stably transfected cell lines. With transfection of a selectable marker linked to a nonselectable target gene (or by cotransfection of the two unlinked genes), high‐level expression of the desired gene is obtained by selecting for ...
Kingston, Robert E., Kaufman, Randal J., Bebbington, C.R., Rolfe, M.R.   +3 more
openaire   +3 more sources

The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells [PDF]

, 2005
The polarized distribution of Na+,K+-ATPase plays a paramount physiological role, because either directly or through coupling with co- and countertransporters, it is responsible for the net movement of, for example, glucose, amino acids, Ca2+, K+, Cl ...
Balda, MS, Cereijido, M, Contreras, RG, Lazaro, A, Matter, K, Moreno, J, Roldan, ML, Shoshani, L   +7 more
core  

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment

AIMS Bioengineering, 2021
Cell growth on three-dimensional objects is of high interest for bioprocess engineering of adherent cells, tissue engineering and other biomedical applications.
Sven Sölmann , Anke Rattenholl, Hannah Blattner, Guido Ehrmann, Frank Gudermann, Dirk Lütkemeyer, Andrea Ehrmann   +6 more
doaj   +1 more source

Serine one-carbon catabolism with formate overflow [PDF]

, 2016
Serine catabolism to glycine and a one-carbon unit has been linked to the anabolic requirements of proliferating mammalian cells. However, genome-scale modeling predicts a catabolic role with one-carbon release as formate. We experimentally prove that in
Athineos, Dimitris, Blyth, Karen, Gottlieb, Eyal, Kamphorst, Jurre J., Labuschagne, Christiaan Fred, Mackay, Gillian M., Maddocks, Oliver, Meiser, Johannes, Tumanov, Sergey, Van Den Broek, Niels, Vazquez, Alexei, Vousden, Karen   +11 more
core   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Development of technology for culturing a cell line producing a single-domain antibody fused with the Fc fragment of human IgG1

Тонкие химические технологии
Objectives. To develop an effective technology for the cultivation of Chinese hamster ovary (CHO) cells stably producing GamP2C5 antibody which is a component I of the GamCoviMab candidate drug for emergency prevention and therapy of  infection caused by
D. S. Polyansky, E. I. Ryabova, A. A. Derkaev, N. S. Starkov, I. S. Kashapova, D. V. Shcheblyakov, A. P. Karpov, I. B. Esmagambetov   +7 more
doaj   +1 more source

Recombinant Vgr-1/BMP-6-expressing tumors induce fibrosis and endochondral bone formation in vivo. [PDF]

, 1994
Members of the TGF-beta superfamily appear to modulate mesenchymal differentiation, including the processes of cartilage and bone formation. Nothing is yet known about the function of the TGF-beta-related factor vgr-1, also called bone morphogenetic ...
Derynck, R, Gitelman, SE, Kobrin, MS, Lee, A, Lopez, AR, Ye, JQ   +5 more
core  

The role of diacylglycerol lipase in constitutive and angiotensin AT(1) receptor-stimulated cannabinoid CB1 receptor activity [PDF]

, 2018
The cannabinoid CB1 receptor (CB1R) is,a G protein-coupled receptor, which couples to the G(i/o) family of heterotrimeric G proteins. The receptor displays both basal and agonist-induced signaling and internalization.
Azpiazu, Bisogno, Bisogno, Bisogno, Bouaboula, Devane, Freund, Hentges, Howlett, Hunyady, Leterrier, Leterrier, MacLennan, Makara, Matsuda, Mechoulam, Neu, Pacher, Pan, Pertwee, Randall, Rinaldi-Carmona, Spaöt, Turu, Vasquez, Vinod, Zhu   +26 more
core   +1 more source