Results 31 to 40 of about 77,065 (293)
Improvement of Technology of Cholera Toxin B-Subunit Production
Проблемы особо опасных инфекций, 2015 Consideration is given to implementation of state-of-the-art filtration technologies for up-scaled manufacturing of cholera toxin B-subunit, produced by recombinant Vibrio cholerae non O1 KM93 strain. Selected are micro- and ultra-filtration membranes to
A. V. Komissarov +8 more
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Binding of NAD+ by cholera toxin [PDF]
Biochemical Journal, 1987 1. The Km for NAD+ of cholera toxin working as an NAD+ glycohydrolase is 4 mM, and this is increased to about 50 mM in the presence of low-Mr ADP-ribose acceptors. Only molecules having both the adenine and nicotinamide moieties of NAD+ with minor alterations in the nicotinamide ring can be competitive inhibitors of this reaction. 2.
Tamara S. Galloway, S van Heyningen
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Проблемы особо опасных инфекций, 2015 Put forward is an efficient method for manufacturing cholera toxin B-subunit. Its advantages are relative simplicity and economy feasibility, as well as maximum output of the purified B-subunit, absolutely free from toxic A-subunit contaminant.
T. L. Zakharova +2 more
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Cell Propagation of Cholera Toxin CTA ADP-Ribosylating Factor by Exosome Mediated Transfer [PDF]
, 2018 In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population.
Boussadia, Zaira +11 more
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Production of putative enhanced oral cholera vaccine strains that express toxin-coregulated pilus. [PDF]
PLoS ONE, 2017 The use of whole cell killed (WCK) oral cholera vaccines is an important strategy for cholera prevention in endemic areas. To overcome current vaccine limitations, we engineered strains of V.
Caitlyn A Hauke, Ronald K Taylor
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Photolabelling of cholera toxin by NAD+ [PDF]
Biochemical Journal, 1987 When cholera toxin is incubated under u.v. light with NAD+ labelled in either the adenine or the nicotinamide moiety, radioactivity becomes covalently bound to the protein. The reaction is specific for cholera toxin, and is inhibited by excess unlabelled NAD+ or NAD analogues. Only the active A 1 chain of the toxin is labelled.
S van Heyningen +2 more
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Microcavity supported lipid membranes: versatile platforms for building asymmetric lipid bilayers and for protein recognition [PDF]
, 2019 Microcavity supported lipid bilayers (MSLB) are contact-free membranes suspended across aqueousfilled pores that maintain the lipid bilayer in a highly fluidic state and free from frictional interactions with substrate.
Berselli, Guilherme +4 more
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Actions of cholera toxin and the prevention and treatment of cholera [PDF]
Nature, 1981 The drastic intestinal secretion of fluid and electrolytes that is characteristic of cholera is the result of reasonably well understood cellular and biochemical actions of the toxin secreted by Vibrio cholerae. Based on this understanding it is possible to devise new techniques for the treatment and prophylaxis of cholera to complement those based on ...
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A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells [PDF]
, 2008 Mannose 6-phosphate receptors (MPRs) are transported from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway. This process requires Rab9 guanosine triphosphatase (GTPase) and the putative tether GCC185.
Espinosa, Eric +2 more
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Quantitative prediction of multivalent ligand–receptor binding affinities for influenza, cholera, and anthrax inhibition [PDF]
, 2018 Multivalency achieves strong, yet reversible binding by the simultaneous formation of multiple weak bonds. It is a key interaction principle in biology and promising for the synthesis of high-affinity inhibitors of pathogens. We present a molecular model
Liese, Susanne, Netz, Roland R.
core +2 more sources