Results 61 to 70 of about 45,647 (229)

Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids

, 2021
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent fusion events require additional protein machinery.
Taras, Sych, W Bruce, Turnbull, Michael E, Webb, Gemma C, Wildsmith, Winfried, Römer, Maren, Thomsen, Daniel J, Williamson, Wenyue, Dai, Lina, Siukstaite, Sarah, Wehrum, James F, Ross, Thomas R, Branson, Josef, Madl   +12 more
core   +1 more source

In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli

Toxins, 2022
Non-toxic derivatives of the cholera toxin are extensively used in neuroscience, as neuronal tracers to reveal the location of cells in the central nervous system.
Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner, Winfried Römer, W. Bruce Turnbull, Juergen Mairhofer   +7 more
doaj   +1 more source

Binding of NAD+ by cholera toxin [PDF]

Biochemical Journal, 1987
1. The Km for NAD+ of cholera toxin working as an NAD+ glycohydrolase is 4 mM, and this is increased to about 50 mM in the presence of low-Mr ADP-ribose acceptors. Only molecules having both the adenine and nicotinamide moieties of NAD+ with minor alterations in the nicotinamide ring can be competitive inhibitors of this reaction. 2.
T S, Galloway, S, van Heyningen
openaire   +2 more sources

Integrating One Health to Mitigate the Emergence and Spread of Antimicrobial Resistance in Livestock and Aquaculture

Animal Research and One Health, EarlyView.
Antimicrobial resistance (AMR) is an escalating global threat driven by antimicrobial use in aquaculture and livestock. Resistant pathogens and genes can spread across humans, animals, and the environment through interconnected ecosystems. Using a One Health approach, this review emphasizes antimicrobial stewardship, regulatory strengthening, enhanced ...
Mir Mohammad Ali, Delower Hossain, Ridwan Olamilekan Adesola, Anum Hanif, Shamsaldeen Ibrahim Saeed, Muhammad Abdul Mannan, Anoar Jamai Masroure, Kazi Estieque Alam, Md Kamal Hossain, Sabiha Zarin Tasnim Bristi, Md Jisan Ahmed   +10 more
wiley   +1 more source

Cholera toxin affects nuclear ADP-ribosylation in GH1 cells

, 2022
Incubation of GH1 cells with cholera toxin for 24 h inhibits [32P]ADP-ribose incorporation into histones and non-histone nuclear proteins by more than 50%. The toxin produces a generalized decrease of incorporation into all protein acceptors and into the
Aranda, Ana, Samuels, Herbert, Copp, Richard, Pascual, Ángel   +3 more
core   +1 more source

Force stimulation promotes nerve regeneration by restoring cellular energy

BMEMat, EarlyView.
Mechanical stimulation can help nerves regenerate in various ways. We developed two devices (a piezo‐motor‐driven stretching device and a SAW‐based actuator) to apply mechanical stimulation to sciatic nerve and DRG neurons. Our study shows that appropriate mechanical force stimulation can promote regeneration by restoring the energy supply to the ...
Zhe Wang, Chen Cai, Hetao Xie, Yupeng Yang, Lei Li, Nik Ahmad Nizam Nik Malek, Wan Hairul Anuar Kamaruddin, Meiwan Chen, Yuanhua Sang, Bing Ji, Zenan Wang   +10 more
wiley   +1 more source

Cholera toxin binding to GM1.

, 2014
(A) HEK293 cells transiently expressing ABCG1-GFP, ABCG1-KM-GFP, ABCG4-GFP, or ABCG4-KM-GFP were incubated with Alexa555-conjugated cholera toxin on ice and fixed with 4% paraformaldehyde.
Aya Kobayashi (642543), Michinori Matsuo (169167), Shiho Ito (642542), Kazumitsu Ueda (169170), Osamu Sano (642541), Yuji Shimizu (174369), Reiko Kato (372943), Noriyuki Kioka (169174), Yasuhisa Kimura (642544), Kentaro Hanada (207598)   +9 more
core   +1 more source

Photolabelling of cholera toxin by NAD+ [PDF]

Biochemical Journal, 1987
When cholera toxin is incubated under u.v. light with NAD+ labelled in either the adenine or the nicotinamide moiety, radioactivity becomes covalently bound to the protein. The reaction is specific for cholera toxin, and is inhibited by excess unlabelled NAD+ or NAD analogues. Only the active A 1 chain of the toxin is labelled.
T S, Galloway, R M, Tait, S, van Heyningen   +2 more
openaire   +2 more sources

Chemotherapeutic Potential of Fluorouracil‐Platinum (IV) Prodrugs Against Cisplatin‐Resistant Colorectal Cancer Cells

Chemistry – A European Journal, EarlyView.
ABSTRACT Fluorouracil‐platinum(IV) prodrugs represent a novel class of multimechanistic chemotherapeutics with enhanced anticancer potential. The prodrugs PtIVP‐5FUMeOBut and PtIV56‐5FUMeOBut were actualized by derivatising the clinical drug 5‐fluorouracil (5FU) and coordinating it to platinum(IV) complexes, leveraging the established cytotoxicity of ...
Maria George Elias, Aleen Khoury, Shadma Fatima, Timothy J. Mann, Shawan Karan, Meena Mikhael, Paul de Souza, Christopher P. Gordon, Kieran F. Scott, Janice R. Aldrich‐Wright   +9 more
wiley   +1 more source

Cholera toxin facilitates calcium transport in jejunal brush border vesicles

, 1986
Cholera toxin is very well characterized in terms of the activation of adenylate cyclase. In some systems, however, this cyclase activation does not seem to account for all of the physiological responses to the toxin.
David D. Maenz, G. W. Forsyth
core   +1 more source