Results 81 to 90 of about 222,465 (345)

Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

Advanced Functional Materials, EarlyView.
CRISPR machinery in diverse molecular formats (DNA, RNA, and ribonucleic protein) is complexed into nanoparticles with the cell‐friendly arginine‐alanine‐leucine‐alanine (RALA) cell‐penetrating peptide. Nanoparticles are delivered to primary mesenchymal stem cells ex vivo or locally in vivo to facilitate multimodal CRISPR gene editing. This RALA‐CRISPR
Joshua P. Graham, Jose Gabriel Castro, Lisette C. Werba, Joseph Amitrano, Luke Fardone, Kevin P. Francis, Anand Ramamurthi, Michael J. Layden, Helen O. McCarthy, Tomas Gonzalez‐Fernandez   +9 more
wiley   +1 more source

3D Culture of Chondrocytes in Gelatin Hydrogels with Different Stiffness

Polymers, 2016
Gelatin hydrogels can mimic the microenvironments of natural tissues and encapsulate cells homogeneously, which makes them attractive for cartilage tissue engineering.
Xiaomeng Li, Shangwu Chen, Jingchao Li, Xinlong Wang, Jing Zhang, N. Kawazoe, Guoping Chen   +6 more
semanticscholar   +1 more source

Adalimumab‐Poloxamer Conjugate for Bio‐Better: Enhanced Stability and Function

Advanced Functional Materials, EarlyView.
Antibody‐polymer conjugates, particularly poloxamer conjugates, enhance antibody stability by improving tolerance to physicochemical stress and attenuating proteolysis by proteases. Furthermore, the higher affinity observed with poloxamer conjugation compared to standard PEGylation results in improved therapeutic efficacy in rheumatoid arthritis mouse ...
Jaewon Roh, Sanghee Lee, Soomin Cho, Kun Na   +3 more
wiley   +1 more source

Cultured Horse Articular Chondrocytes in 3D-Printed Chitosan Scaffold With Hyaluronic Acid and Platelet Lysate

Frontiers in Veterinary Science, 2021
Three-dimensional (3D) printing has gained popularity in tissue engineering and in the field of cartilage regeneration. This is due to its potential to generate scaffolds with spatial variation of cell distribution or mechanical properties, built with a ...
Elena De Angelis, Roberta Saleri, Paolo Martelli, Lisa Elviri, Annalisa Bianchera, Carlo Bergonzi, Marta Pirola, Roberta Romeo, Melania Andrani, Valeria Cavalli, Virna Conti, Ruggero Bettini, Benedetta Passeri, Francesca Ravanetti, Paolo Borghetti   +14 more
doaj   +1 more source

SOST/Sclerostin Improves Posttraumatic Osteoarthritis and Inhibits MMP2/3 Expression After Injury. [PDF]

, 2018
Patients with anterior cruciate ligament (ACL) rupture are two times as likely to develop posttraumatic osteoarthritis (PTOA). Annually, there are ∼900,000 knee injuries in the United States, which account for ∼12% of all osteoarthritis (OA) cases.
Baek, Balemans, Baron, Bouaziz, Chan, Chang, Christiansen, Collette, Collette, Dejour, Glasson, Goldring, Hwang, Itin, Kapoor, Karlsson, Li, Li, Livak, Lockwood, Loeser, Lohmander, Loots, Mabey, Ovcharenko, Ratzlaff, Reppe, Rigoglou, Roudier, Sokolove, Swarnkar, Trapnell, Trapnell, Trapnell, van Dinther, Velasco, Wehmeyer, Zhu   +37 more
core   +2 more sources

Synergistic Osteogenesis After Co‐Administration of cmRNAs Encoding BMP‐2 and BMP‐7 Utilizing a Transcript‐Activated Matrix

Advanced Functional Materials, EarlyView.
This study demonstrates that the dual delivery of BMP‐2/‐7 coding cmRNAs for bone healing is demonstrated as feasible, safe, and highly osteogenic. Compared to single BMP‐2 or BMP‐7 cmRNAs, the combination enhances the production of both mineral and organic components of the extracellular matrix when delivered using a collagen‐HA scaffold, supporting ...
Claudia Del Toro Runzer, Joanna M. Sadowska, Nadia Roumans, Christian Plank, Christopher H. Evans, Fergal J. O'Brien, Martijn van Griensven, Elizabeth R. Balmayor   +7 more
wiley   +1 more source

Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo

PLoS ONE, 2017
Cartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of a process capable of creating a patient-specific cartilage framework would be a major breakthrough.
Peter Apelgren, M. Amoroso, A. Lindahl, C. Brantsing, N. Rotter, P. Gatenholm, L. Kölby   +6 more
semanticscholar   +1 more source

Wettability influences cell behavior on superhydrophobic surfaces with different topographies [PDF]

, 2012
Surface wettability and topography are recognized as critical factors influencing cell behavior on biomaterials. So far only few works have reported cell responses on surfaces exhibiting extreme wettability in combination with surface topography.
Apeldoorn, A.A. van, Blitterswijk, C.A. van, Karperien, H.B.J., Lourenco, B.N., Mano, J.F., Marchioli, G., Reis, R.L., Song, W   +7 more
core   +3 more sources

Biomaterial Strategies for Targeted Intracellular Delivery to Phagocytes

Advanced Functional Materials, EarlyView.
Phagocytes are essential to a functional immune system, and their behavior defines disease outcomes. Engineered particles offer a strategic opportunity to target phagocytes, harnessing inflammatory modulation in disease. By tuning features like size, shape, and surface, these systems can modulate immune responses and improve targeted treatment for a ...
Kaitlyn E. Woodworth, Neal I. Callaghan, Locke Davenport Huyer   +2 more
wiley   +1 more source

HOX Gene Expressions in Cultured Articular and Nasal Equine Chondrocytes

Animals, 2021
Osteoarthritis the quality and span of life in horses. Previous studies focused on nasal cartilage as a possible source for autologous chondrocyte implantation (ACI) in cartilage defects in humans. “HOX gene-negative” nasal chondrocytes adapted articular
Christiane Storch, Herbert Fuhrmann, Axel Schoeniger   +2 more
doaj   +1 more source