Results 91 to 100 of about 4,869 (131)

Mass Spectrometric Characterization of the β-Subunit of Human Chorionic Gonadotropin

Journal of Mass Spectrometry, 1997
A high-performance liquid chromatographic/electrospray mass spectrometric (HPLC/MS) technique is described for the characterization of the beta-subunit of the glycopeptide human chorionic gonadotropin (hCG). The beta-subunit of hCG was dissociated from the alpha-subunit using 0.1% trifluoroacetic acid (TFA) and separated by reversed-phase HPLC using a ...
Larry D. Bowers, Chuan-Liang Liu
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Identification and selective destruction of shared epitopes in human chorionic gonadotropin beta subunit

Journal of Reproductive Immunology, 1996
The feasibility of producing epitope-specific antigens by mutation of the gene is demonstrated, the aim being to eliminate unwanted surface epitopes yet allowing the natural folding of the protein to maintain the desired epitope(s). The model protein is the beta subunit of human chorionic gonadotropin (hCG beta) which previously has been used as an ...
Torben Lund, Alison M. Jackson, Thomas Klonisch, Neil W. Isaacs, Adrian J. Lapthorn, Peter Berger, Ivan M. Roitt, Peter J. Delves   +7 more
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Expression of α- and β-human chorionic gonadotropin subunits in cultured human cells [PDF]

In Vitro Cellular & Developmental Biology, 1990
We surveyed several human cell lines for production of alpha- and beta-human chorionic gonadotropin (hCG) under a variety of conditions known to induce gene expression. alpha- and beta-hCG subunits were monitored in culture media by specific radioimmunoassays and were shown to be quite sensitive to serum refeeding and growth state of all cell types ...
Robert J. Shmookler Reis, Glenn D. Braunstein, Jones W. Hardin, Richard A. Jones, Samuel Goldstein   +4 more
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Human chorionic gonadotropin: Amino acid sequence of the α and β subunits

Biochemical and Biophysical Research Communications, 1972
Summary The linear amino acid sequences of the α and β subunits of human chorionic gonadotropin have been determined. HCG-α has 92 and HCG-β 139 amino acid residues. There is more homology between HCG-α and ovine LH-α than between HCG-β and ovine LH-β.
Robert B. Carlsen, N. Swaminathan, Ronald Bellisario, Om P. Bahl   +3 more
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Monoclonal Antibody against Freeβ-Subunit of Human Chorionic Gonadotropin

Hybridoma and Hybridomics, 2002
Spleen cells from BALB/c mice immunized with free native human chorionic gonadotropin hormone beta-subunit (beta hCG) were fused with mouse myeloma cells (P3/X63-Ag8) and one hybridoma secreting monoclonal antibodies (MAbs), was obtained. This hybridoma specifically recognizes beta hCG and does not cross-react with other human glycoprotein hormones ...
Ileana B. Baluja Conde, Luisa Martínez, Adys I. Brito Moreno, Milenen Hernández, Isis Amores Sánchez, Orlando Zalueta Rodríguez, Carmen Acosta Bas, Antonio Melchor Rodríguez   +7 more
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Evolution of the genes for the β subunits of human chorionic gonadotropin and luteinizing hormone

Nature, 1984
Nucleotide sequence comparisons of the single gene for the human luteinizing hormone gene beta subunit with two of the seven genes for the human chorionic gonadotropin beta subunit suggest that the beta human chorionic gonadotropin genes have evolved from an ancestral beta luteinizing hormone gene by a series of selected changes with very little ...
Nikos C. Vamvakopoulos, Karen Talmadge, John C. Fiddes   +2 more
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A Radioimmunoassay for the Core Fragment of the Human Chorionic Gonadotropinβ-Subunit

The Journal of Clinical Endocrinology & Metabolism, 1988
We developed a RIA for the beta-core fragment of hCG that is excreted in the urine of pregnant women and some patients with cancer. We purified beta-core from crude commercial preparations of hCG (of which beta-core is a major constituent) derived from pregnancy urine and used this purified beta-core material to immunize rabbits.
Bruce C. Nisula, Robert E. Wehmann, Catherine Blacker, Antoine H. Akar, Diana L. Blithe   +4 more
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Detection and Quantitation of the β-Subunit of Human Chorionic Gonadotropin in Serum by Radioimmunoassay

Fertility and Sterility, 1980
Qualitative and quantitative radioimmunoassays (RIA) specific for the beta-subunit of human chorionic gonadotropin (HCG) produced by Wampole Laboratories and Monitor Science Corporation were compared in a study involving the sera from 213 individuals. Qualitative assays from both manufacturers were found to be equivalent in clinical specificity (99.2%).
Beverly Barrett, William L. Boyko
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Monoclonal Antibodies Against Immunodeterminants Associated with the Alpha and Beta Subunits of Human Chorionic Gonadotropin

Hybridoma, 1982
Spleen cells of BALB/c mice immunized with human chorionic gonadotropin (HCG), a glycopeptide hormone composed of two nonidentical alpha and beta subunits, were fused with NS-1 mouse myeloma cells. Two hybrid cell lines, cone PC2 and clone PD3 secreting monoclonal antibodies (McAb) to HCG were isolated.
Michael Inbar, Lynn Wang, Nurit Harpaz, Nadia Rahamim, Charles S. Hexter   +4 more
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Characterization of a Discrete Degradation Product of the Human Chorionic Gonadotropin β-Subunit in Humans

The Journal of Clinical Endocrinology & Metabolism, 1980
We have investigated the different forms of immunoreactive hCGβ excreted in the urine of normal volunteers after iv administration of highly purified hCG β-subunit. Chromatography of urine on Sephadex G-100 revealed two discrete peaks ofhCGβ immunoreactivity as measured with antiserum to hCGβ (SB6).
Bruce C. Nisula, Robert E. Wehmann
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