Results 51 to 60 of about 2,183,040 (299)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

3D Analysis of chromosome architecture: advantages and limitations with SEM [PDF]

, 2005
Three-dimensional mitotic plant chromosome architecture can be investigated with the highest resolution with scanning electron microscopy compared to other microscopic techniques at present.
Formanek, H., Schroeder-Reiter, E., Wanner, Gerhard   +2 more
core   +1 more source

Nuclear architecture explored by live-cell fluorescence microscopy using laser and ion microbeam irradiation. [PDF]

, 2010
Nuclear architecture is a biological field of research that studies the spatio-temporal organization of the components within cell nuclei. Since nuclei are the organelles that harbor the genome and epigenome, they are the place where most of the genetic ...
Strickfaden, Hilmar
core   +1 more source

Extensive Chromatin Structure-Function Associations Revealed by Accurate 3D Compartmentalization Characterization

Frontiers in Cell and Developmental Biology, 2022
A/B compartments are observed in Hi-C data and coincide with eu/hetero-chromatin. However, many genomic regions are ambiguous under A/B compartment scheme.
Zi Wen, Zi Wen, Weihan Zhang, Quan Zhong, Quan Zhong, Jinsheng Xu, Jinsheng Xu, Chunhui Hou, Zhaohui Steve Qin, Li Li, Li Li, Li Li   +11 more
doaj   +1 more source

Chromatin architecture and nuclear RNA. [PDF]

Proceedings of the National Academy of Sciences, 1989
The maintenance of normal chromatin morphology requires ongoing RNA synthesis. We have examined the role of RNA in chromatin organization, using selective detergent extraction of cells, RNA synthesis inhibitors, and enzymatic digestion of nuclear RNA.
J A, Nickerson, G, Krochmalnic, K M, Wan, S, Penman   +3 more
openaire   +2 more sources

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

H2A.Z facilitates access of active and repressive complexes to chromatin in embryonic stem cell self-renewal and differentiation. [PDF]

, 2013
SummaryChromatin modifications have been implicated in the self-renewal and differentiation of embryonic stem cells (ESCs). However, the function of histone variant H2A.Z in ESCs remains unclear.
Northrup, D., Levens, D., Tang, Q., Liu, Chengyu, Zhao, Keji, Hu, G., Cui, K., Wang, Chaochen, Hu, Gangqing, Crane-Robinson, Colyn, Ge, Kai, Northrup, Daniel, Wang, C., Tang, Qingsong, Zhao, K., Levens, David, Liu, C., Cui, Kairong, Ge, K.   +18 more
core   +1 more source

BRD4 Phosphorylation Regulates the Structure of Chromatin Nanodomains

Cells
The interplay between chromatin structure and phase-separating proteins is an emerging topic in cell biology with implications for understanding disease states.
Clayton Seitz, Donghong Fu, Mengyuan Liu, Hailan Ma, Jing Liu   +4 more
doaj   +1 more source

Dynamic chromatin architecture of the porcine adipose tissues with weight gain and loss

Nature Communications, 2023
Using an adult female miniature pig model with diet-induced weight gain/weight loss, we investigated the regulatory mechanisms of three-dimensional (3D) genome architecture in adipose tissues (ATs) associated with obesity.
Long Jin, Danyang Wang, Jiaman Zhang, Pengliang Liu, Yujie Wang, Yu Lin, Can Liu, Ziyin Han, Keren Long, Diyan Li, Yu Jiang, Guisen Li, Yu Zhang, Jingyi Bai, Xiaokai Li, Jing Li, Lu Lu, Fanli Kong, Xun Wang, Hua Li, Zhiqing Huang, Jideng Ma, Xiaolan Fan, Linyuan Shen, Li Zhu, Yanzhi Jiang, Guoqing Tang, Bin Feng, Bo Zeng, Liangpeng Ge, Xuewei Li, Qianzi Tang, Zhihua Zhang, Mingzhou Li   +33 more
doaj   +1 more source