Tagging of MADS domain proteins for chromatin immunoprecipitation
BMC Plant Biology, 2007 Background Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns.
van Zuijlen Lisette GC +4 more
doaj +1 more source
Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus [PDF]
, 1996 The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene silencing. We investigated their binding specificity at the constitutively methylated region of a CpG island containing the ...
Auriol, Emilie +3 more
core +2 more sources
Magnetic Beads Make Chromatin Immunoprecipitation Faster and Easier
BioTechniques, 2007 Active Motif's ChIP-IT™ Express Kits use protein G-coated magnetic beads instead of the traditionally used agarose beads to make chromatin immunoprecipitation (ChIP) faster and better than ever before.
John Heyman +3 more
doaj +1 more source
Direct identification of insulator components by insertional chromatin immunoprecipitation. [PDF]
PLoS ONE, 2011 Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. However, non-biased methods to identify molecules bound to specific genomic loci in vivo are limited ...
Toshitsugu Fujita, Hodaka Fujii
doaj +1 more source
A requirement for STAG2 in replication fork progression creates a targetable synthetic lethality in cohesin-mutant cancers. [PDF]
, 2019 Cohesin is a multiprotein ring that is responsible for cohesion of sister chromatids and formation of DNA loops to regulate gene expression. Genomic analyses have identified that the cohesin subunit STAG2 is frequently inactivated by mutations in cancer.
Ashworth, Alan +4 more
core +2 more sources
Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells
Bio-Protocol, 2013 Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA sequences ...
Pia Giovannelli +2 more
doaj +1 more source
HTRIdb: an open-access database for experimentally verified human transcriptional regulation interactions [PDF]
, 2012 Background: The modeling of interactions among transcription factors (TFs) and their respective target genes (TGs) into transcriptional regulatory networks is important for the complete understanding of regulation of biological processes.
Luiz A. Bovolenta +2 more
core +4 more sources
Fast chromatin immunoprecipitation assay [PDF]
Nucleic Acids Research, 2006 Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples.
Nelson, Joel D. +3 more
openaire +2 more sources
Assessing the Epigenetic Status of Human Telomeres [PDF]
, 2019 The epigenetic modifications of human telomeres play a relevant role in telomere functions and cell proliferation. Therefore, their study is becoming an issue of major interest.
Vaquero Sedas, María Isabel +1 more
core +1 more source
Impact of chromatin structures on DNA processing for genomic analyses. [PDF]
PLoS ONE, 2009 Chromatin has an impact on recombination, repair, replication, and evolution of DNA. Here we report that chromatin structure also affects laboratory DNA manipulation in ways that distort the results of chromatin immunoprecipitation (ChIP) experiments. We
Leonid Teytelman +6 more
doaj +1 more source