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Reverse Chromatin Immunoprecipitation (R-ChIP)
DNA-protein interactions play fundamental roles in diverse biological functions. The gene-centered method is used to identify the upstream regulators of defined genes. In this study, we developed a novel method for capturing the proteins that bind to certain chromatin fragments or DNA sequences, which is called reverse chromatin immunoprecipitation (R ...Xuejing, Wen, Yucheng, Wang
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Chromatin Immunoprecipitation (ChIP) Analysis of Protein–DNA Interactions
Cold Spring Harbor Protocols, 2018Here we describe chromatin immunoprecipitation (ChIP), a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function. This approach allows us to determine the distribution of histone modifications (e.g., acetylation, methylation), the deposition of histone variants (H2AZ, H3.3, etc.), and the location of ...
Kim, Tae Hoon, Dekker, Job
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Chop it, ChIP it, check it: the current status of chromatin immunoprecipitation
Frontiers in Bioscience, 2008Our understanding of the significance of interactions of proteins with DNA in the context of gene expression, cell differentiation or to some extent disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP has been widely used to map the localization of post-translationally modified histones or histone variants on
Philippe, Collas, John Arne, Dahl
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Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)
2011Telomere Chromatin Immunoprecipitation (ChIP) is an experimental method used to determine whether proteins are associated with telomere DNA inside the nucleus of cells or tissues. Telomere-associated proteins are covalently cross-linked to telomere DNA, and then immunoprecipitated using a specific antibody for the protein. This method has become one of
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Using Chromatin Immunoprecipitation (ChIP) to Study the Chromatin State inDrosophila
Cold Spring Harbor ProtocolsThe chromatin state plays an important role in regulating gene expression, which affects organismal development and plasticity. Proteins, including transcription factors, chromatin modulatory proteins, and histone proteins, usually with modifications, interact with gene loci involved in cellular differentiation, function, and modulation.
Chengcheng, Du, Pelin, Volkan
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ChIP-re-ChIP: Co-occupancy Analysis by Sequential Chromatin Immunoprecipitation
2017Chromatin immunoprecipitation (ChIP) exploits the specific interactions between DNA and DNA-associated proteins. It can be used to examine a wide range of experimental parameters. A number of proteins bound at the same genomic location can identify a multi-protein chromatin complex where several proteins work together to regulate gene transcription or ...
Timothy V, Beischlag +2 more
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A rapid micro chromatin immunoprecipitation assay (ChIP)
Nature Protocols, 2008Interactions of proteins with DNA mediate many critical nuclear functions. Chromatin immunoprecipitation (ChIP) is a robust technique for studying protein-DNA interactions. Current ChIP assays, however, either require large cell numbers, which prevent their application to rare cell samples or small-tissue biopsies, or involve lengthy procedures.
John Arne, Dahl, Philippe, Collas
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Chromatin Preparation from Frozen Tissues for Chromatin Immunoprecipitation (ChIP) Assays
Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a crucial method for examining transcription factor binding and histone modifications across the entire genome. This is a key step in deciphering the complex mechanisms that control cancer immunosurveillance.Virginia López, Martínez +1 more
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Chromatin immunoprecipitation (ChIP) assay
Protocol Exchange, 2009Zhongfu Ni +3 more
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