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Protocol for the fast chromatin immunoprecipitation (ChIP) method
Nature Protocols, 2006Chromatin and transcriptional processes are among the most intensively studied fields of biology today. The introduction of chromatin immunoprecipitations (ChIP) represents a major advancement in this area. This powerful method allows researchers to probe specific protein-DNA interactions in vivo and to estimate the density of proteins at specific ...
Joel D, Nelson +2 more
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Chromatin Immunoprecipitation (ChIP) to Study DNA–Protein Interactions
2021Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements). As ChIP provides a snapshot of in vivo DNA-protein interactions, it lends insight to the mechanisms of gene expression and genome regulation.
Eliza C, Small +5 more
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Chromatin Immunoprecipitation (ChIP) with Erythroid Samples
2017Chromatin immunoprecipitation (ChIP) allows determination of the locations to which a select protein is bound in chromatin. Chemical crosslinking of DNA and protein with bi-functional reagents such as formaldehyde and precipitation of the protein with a specific antibody permit PCR amplification (ChIP) or sequencing (ChIP-seq) to identify the bound ...
Ivan, Krivega, Ann, Dean
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Chromatin Immunoprecipitation (ChIP)
2020Chromatin immunoprecipitation or ChIP is an excellent method of investigation of the specific protein interaction and its altered forms with DNA region. These interactions have a significant role in various cellular processes such as replication, transcription, DNA damage repair, genome stability, gene regulation and segregation at mitosis.
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Fish’n ChIPs: Chromatin Immunoprecipitation in the Zebrafish Embryo
2009Chromatin immunoprecipitation (ChIP) is arguably the assay of choice to determine the genomic localization of DNA- or chromatin-binding proteins, including post-translationally modified histones, in cells. The increasing importance of the zebrafish, Danio rerio, as a model organism in functional genomics has recently sparked investigations of ChIP ...
Leif C, Lindeman +3 more
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Targeted discovery tools: proteomics and chromatin immunoprecipitation‐on‐chip
BJU International, 2005Despite the availability of several completely sequenced genomes, we are still, for the most part, ignorant about how genes interact and regulate each other within a given cell type to specify identity, function and cellular memory. A realistic model of cellular regulation based on current knowledge indicates that many interacting networks operate at ...
Guezennec, X.S. le +6 more
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Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei
2020Understanding the binding of regulatory proteins to their cognate genomic sites is an important step in deciphering transcriptional networks such as the circadian oscillator. Chromatin immunoprecipitation (ChIP) enables the detection and temporal analysis of such binding events in vivo.
Sara S, Fonseca Costa +1 more
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Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans
2016Chromatin immunoprecipitation (ChIP) is a widely used technique which can determine the in vivo association of a specific protein on a particular DNA locus in the genome. In this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest.
Sreyoshi, Mitra +3 more
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Yeast Chromatin Immunoprecipitation (ChIP) Assay
Cold Spring Harbor Protocols, 2007INTRODUCTIONThis protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of this assay.
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Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP
2009Chromatin immunoprecipitation has been widely used to determine the status of histone covalent modifications and also to investigate DNA-protein and protein-protein associations to a particular genomic location in vivo. Generally, DNA regulatory elements nucleate the interaction of several transcription factors in conjunction with ubiquitous and/or ...
Mayra, Furlan-Magaril +2 more
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