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Chromatin Immunoprecipitation (ChIP) in Schizosaccharomyces pombe
Cold Spring Harbor Protocols, 2016Chromatin immunoprecipitation (ChIP), the cross-linking of chromatin followed by immunoprecipitation with antibodies against a chromatin target, is a key method for measuring association of proteins with a specific genomic region(s). As a negative control, a mock ChIP experiment in which no antibody is added to the immunoprecipitation reaction is ...
Hugh P, Cam, Simon, Whitehall
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Chromatin Immunoprecipitation (ChIP)
2020Chromatin immunoprecipitation or ChIP is an excellent method of investigation of the specific protein interaction and its altered forms with DNA region. These interactions have a significant role in various cellular processes such as replication, transcription, DNA damage repair, genome stability, gene regulation and segregation at mitosis.
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Chromatin Immunoprecipitation (ChIP) with Erythroid Samples
2017Chromatin immunoprecipitation (ChIP) allows determination of the locations to which a select protein is bound in chromatin. Chemical crosslinking of DNA and protein with bi-functional reagents such as formaldehyde and precipitation of the protein with a specific antibody permit PCR amplification (ChIP) or sequencing (ChIP-seq) to identify the bound ...
Ivan, Krivega, Ann, Dean
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Protocol for the fast chromatin immunoprecipitation (ChIP) method
Nature Protocols, 2006Chromatin and transcriptional processes are among the most intensively studied fields of biology today. The introduction of chromatin immunoprecipitations (ChIP) represents a major advancement in this area. This powerful method allows researchers to probe specific protein-DNA interactions in vivo and to estimate the density of proteins at specific ...
Joel D, Nelson +2 more
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Chromatin Immunoprecipitation (ChIP) to Study DNA–Protein Interactions
2021Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements). As ChIP provides a snapshot of in vivo DNA-protein interactions, it lends insight to the mechanisms of gene expression and genome regulation.
Eliza C, Small +5 more
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Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP
2009Chromatin immunoprecipitation has been widely used to determine the status of histone covalent modifications and also to investigate DNA-protein and protein-protein associations to a particular genomic location in vivo. Generally, DNA regulatory elements nucleate the interaction of several transcription factors in conjunction with ubiquitous and/or ...
Mayra, Furlan-Magaril +2 more
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Yeast Chromatin Immunoprecipitation (ChIP) Assay
Cold Spring Harbor Protocols, 2007INTRODUCTIONThis protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of this assay.
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Targeted discovery tools: proteomics and chromatin immunoprecipitation‐on‐chip
BJU International, 2005Despite the availability of several completely sequenced genomes, we are still, for the most part, ignorant about how genes interact and regulate each other within a given cell type to specify identity, function and cellular memory. A realistic model of cellular regulation based on current knowledge indicates that many interacting networks operate at ...
Guezennec, X.S. le +6 more
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Reverse Chromatin Immunoprecipitation (R-ChIP)
DNA-protein interactions play fundamental roles in diverse biological functions. The gene-centered method is used to identify the upstream regulators of defined genes. In this study, we developed a novel method for capturing the proteins that bind to certain chromatin fragments or DNA sequences, which is called reverse chromatin immunoprecipitation (R ...Xuejing, Wen, Yucheng, Wang
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Chop it, ChIP it, check it: the current status of chromatin immunoprecipitation
Frontiers in Bioscience, 2008Our understanding of the significance of interactions of proteins with DNA in the context of gene expression, cell differentiation or to some extent disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP has been widely used to map the localization of post-translationally modified histones or histone variants on
Philippe, Collas, John Arne, Dahl
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