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Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes
Methods in Molecular Biology, 2019 Chromatin immunoprecipitation (ChIP) in conjunction with qPCR or next generation sequencing (ChIPseq) is used to detect protein-DNA interaction. Typically, DNA bound to a protein of interest is captured with an antibody against this protein, and DNA is ...
Barbara Di Benedetto, Mira Jakovcevski
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Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)
Methods in Molecular Biology, 2017Telomere chromatin immunoprecipitation (ChIP) is an experimental method used to determine whether proteins are associated with telomere DNA inside the nuclei of cells and tissues. Telomere-associated proteins are first covalently crosslinked to telomere DNA, and then immunoprecipitated using an antibody specific for the protein of interest. This method
Wenbin Ma, Xuyang Feng
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Chromatin Immunoprecipitation for ChIP-chip and ChIP-seq
Methods in Molecular Biology, 2014Bacterial adaptation to given environmental conditions is largely achieved by complex gene regulatory processes. To address the question how and to what extend single transcriptional regulators modulate gene expression, chromatin immunoprecipitation (ChIP) coupled to DNA microarrays (ChIP-chip) or to next-generation sequencing (ChIP-seq) is one of the ...
Susanne Häusler, Sebastian Schulz
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Chromatin Immunoprecipitation (ChIP)
Cold Spring Harbor Protocols, 2009INTRODUCTIONChromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with
Michael F, Carey +2 more
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Fish’n ChIPs: Chromatin Immunoprecipitation in the Zebrafish Embryo
Methods in Molecular Biology, 2009Chromatin immunoprecipitation (ChIP) is arguably the assay of choice to determine the genomic localization of DNA- or chromatin-binding proteins, including post-translationally modified histones, in cells. The increasing importance of the zebrafish, Danio rerio, as a model organism in functional genomics has recently sparked investigations of ChIP ...
Peter Aleström, Philippe Collas
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Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin-Associated Proteins [PDF]
Methods in Molecular Biology, 2009 Disruption of epigenetic regulators of transcription is a central mechanism of oncogenesis. Many of the advances in the understanding of these mechanisms are attributable to the successful development of chromatin immunoprecipitation (ChIP) for in vivo detection of histone modifications as well as chromatin binding regulatory proteins.
Keji Zhao, Thomas A Milne
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DNA-Enrichment Microfluidic Chip for Chromatin Immunoprecipitation
Analytical Chemistry, 2009Chromatin immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting association of individual proteins with specific genomic regions; the technique requires several complex steps and is tedious. In this paper, we develop a microbead-packed microfluidic chip which eliminates most of the laborious, time-consuming, and skill ...
Hyun Jik, Oh +7 more
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