Results 121 to 130 of about 259,952 (309)

Isolation of a vicilin gene from pea (pisum sativum l.), and nuclease sensitivity of seed storage protein genes in pea chromatin [PDF]

, 1986
A library of pea genomic DNA in the bacteriophage vector EMBL3 was screened by hybridisation to cDNAs encoding vicilin, a major storage protein of pea (Pisum sativum L.) seeds.
Sawyer, Rosalind Mary
core  

DNA methylation in chromatin - complexes and mechanisms

, 2010
The DNA in the eukaryotic genome is packaged into chromatin whose basic repeating unit is the nucleosome consisting of 147bp of DNA wrapped around an octameric histone core.
Felle, Max
core   +1 more source

Acetylation of histone H3 at lysine 64 regulates nucleosome dynamics and facilitates transcription [PDF]

, 2014
Post-translational modifications of proteins have emerged as a major mechanism for regulating gene expression. However, our understanding of how histone modifications directly affect chromatin function remains limited.
Holzner, M. (M), Eleni Kallis, Holzner, M., Michaelis, J., Richter, F. (F) M. (M), Ryan, D. (D) P. (P), Feldmann, A. (A), Daniel P Ryan, Owen-Hughes, Tom; id_orcid, Kacem, S., Tropberger, Philipp, Di Cerbo, V., Bannister, A., Kallis, E. (E), Michaelis, Jens, Feldmann, A., Richter, Florian, Martin, Daujat, S. (Sylvain), Robert Schneider, Richter, F., Tropberger, P., Hoerner, Leslie, Richter, Florian Martin, Mittler, G. (G), Emilie Montellier, Di Cerbo, V. (V), Feil, Robert, Bannister, A. (A) J. (J), Vincenzo Di Cerbo, Ryan, D., Kallis, Eleni, Ryan, D. P., Di Cerbo, Vincenzo, Owen-Hughes, T. (T), Leslie Hoerner, Jens Michaelis, Kallis, E., Khochbin, S. (S), Saadi Khochbin, Schneider, Robert, Holzner, Monika, Monika Holzner, Feil, R. (R), Ryan, Daniel, P., Feldmann, Angelika, Tom Owen-Hughes, Owen-Hughes, T., Bannister, A. J., Feil, R., Mohn, F., Hoerner, L., Fabio Mohn, Richter, F. M., Montellier, E. (E), Mittler, G., Schuebeler, D., Montellier, Emilie, Mohn, F. (F), Tropberger, P. (P), Angelika Feldmann, Owen-Hughes, Tom, Kacem, Salim, Mittler, Gerhard, Daujat, Sylvain, Montellier, E., Schneider, R., Dirk Schuebeler, Schuebeler, D. (D), Bannister, Andrew J., Hoerner, L. (L), Khochbin, S., Schneider, R. (Robert), Schuebeler, Dirk, Salim Kacem, Philipp Tropberger, Mohn, Fabio, Florian Martin Richter, Kacem, S. (S), Sylvain Daujat, Ryan, Daniel P., Andrew J Bannister, Khochbin, Saadi, Gerhard Mittler, Michaelis, J. (J), Robert Feil, Daujat, S.   +85 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Characterization of the HIV-1 integrase chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase

Virology Journal, 2010
Background During the early stage of HIV-1 replication, integrase (IN) plays important roles at several steps, including reverse transcription, viral DNA nuclear import, targeting viral DNA to host chromatin and integration.
Yao Xiaojian, Jayappa Kallesh, Ao Zhujun, Zheng Yingfeng   +3 more
doaj   +1 more source

High resolution discovery of chromatin interactions

Nucleic Acids Research, 2018
ABSTRACT Chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) is a method for the genome-wide de novo discovery of chromatin interactions. Existing computational methods typically fail to detect weak or dynamic interactions because they use a peak-calling step that
Guo, Yuchun, Krismer, Konstantin, Closser, Michael, Wichterle, Hynek, Gifford, David K.   +4 more
openaire   +2 more sources

Models incorporating chromatin modification data identify functionally important p53 binding sites

, 2013
Genome-wide prediction of transcription factor binding sites is notoriously difficult. We have developed and applied a logistic regression approach for prediction of binding sites for the p53 transcription factor that incorporates sequence information ...
Barker, Daniel, Iggo, Richard Derek, Lim, Ji-Hyun   +2 more
core   +1 more source

The RNA helicase Rm62 cooperates with SU(VAR)3-9 to re-silence active transcription in Drosophila melanogaster. [PDF]

, 2011
Gene expression is highly dynamic and many genes show a wide range in expression over several orders of magnitude. This regulation is often mediated by sequence specific transcription factors.
Bag, I., M. Janaki Ramaiah, Ramaiah, M.J., Bag, Indira, Irene Vetter, Boeke Joern, Kremmer Elisabeth, Vetter, I., Imhof Axel, Bhadra Utpal, Bhadra, U., Kremmer, E., Boeke, J., Kremmer, Elisabeth, Axel Imhof, Manika Pal-Bhadra, Boeke, Joern, Utpal Bhadra, Pal-Bhadra, Manika, Indira Bag, Vetter, Irene, Vetter Irene, M Janaki Ramaiah, Pal-Bhadra Manika, Imhof, Axel, Pal-Bhadra, M., Joern Boeke, Bhadra, Utpal, Ramaiah, M. Janaki, Imhof, A., Bag Indira, Elisabeth Kremmer, Ramaiah M. Janaki   +32 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Biophysical studies of cholesterol effects on chromatin[S]

Journal of Lipid Research, 2017
Changes in chromatin structure regulate gene expression and genome maintenance. Molecules that bind to the nucleosome, the complex of DNA and histone proteins, are key modulators of chromatin structure.
Isabel T.G. Silva, Vinícius Fernandes, Caio Souza, Werner Treptow, Guilherme M. Santos   +4 more
doaj   +1 more source