Results 41 to 50 of about 7,699,681 (297)

Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

Nature Communications, 2019
Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into ...
Marcus D. Wilson, Ludovic Renault, Daniel P. Maskell, Mohamed Ghoneim, Valerie E. Pye, Andrea Nans, David S. Rueda, Peter Cherepanov, Alessandro Costa   +8 more
doaj   +1 more source

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV

Nature Communications, 2021
Understanding the pathology and immunological response to SARS CoV2 infection in specific patient groups is essential for informing the scientific and clinical handling of infections within these patient populations.
Aljawharah Alrubayyi, Ester Gea-Mallorquí, Emma Touizer, Dan Hameiri-Bowen, Jakub Kopycinski, Bethany Charlton, Natasha Fisher-Pearson, Luke Muir, Annachiara Rosa, Chloe Roustan, Christopher Earl, Peter Cherepanov, Pierre Pellegrino, Laura Waters, Fiona Burns, Sabine Kinloch, Tao Dong, Lucy Dorrell, Sarah Rowland-Jones, Laura E. McCoy, Dimitra Peppa   +20 more
doaj   +1 more source

Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells [PDF]

, 2002
Expression of the gene for the macrophage colony stimulating factor receptor (CSF-1R), c-fms, has been viewed as a hallmark of the commitment of multipotent precursor cells to macrophages.
Bonifer, Constanze, Pieter J.M. Leenen, Himes, R, Clarke, D, Himes, Roy, Riggs, A. D., Bonifer, Constanze; id_orcid, Tagoh, H., Tagoh, Hiromi, Hume, David, Himes, R., Tagoh, H, Hume, D., Leenen, PJ, Roy Himes, Clarke, D., Bonifer, C, Leenen, Pieter J M, Constanze Bonifer, David Hume, Riggs, AD, Bonifer, C., Leenen, P. J. M., Arthur D. Riggs, Leenen, Pieter, Clarke, Deborah, Hiromi Tagoh, Deborah Clarke, Riggs, Arthur D, Hume, D   +29 more
core   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Structural basis of RNAPII transcription on the nucleosome containing histone variant H2A.B

The EMBO Journal
H2A.B is a distant histone H2A variant associated with actively transcribed regions of the genome, suggesting its positive role in promoting transcription.
Munetaka Akatsu, Rina Hirano, Tomoya Kujirai, Mitsuo Ogasawara, Haruhiko Ehara, Shun-ichi Sekine, Yoshimasa Takizawa, Hitoshi Kurumizaka   +7 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Quantitative analysis of chromatin compaction in living cells using FLIM-FRET [PDF]

, 2009
We present a quantitative Forster resonance energy transfer (FRET)-based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell
Angus I. Lamond, Norman, David G., James, John, Lleres, David, Lamond, Angus, I, John James, David Llères, Swift, Sam, David G. Norman, Norman, David, G, Lamond, Angus I.; id_orcid, Sam Swift   +11 more
core   +1 more source

Unconventional metabolites in chromatin regulation

, 2021
Chromatin, the complex of DNA and histone proteins, serves as a main integrator of cellular signals. Increasing evidence links cellular functional to chromatin state.
Alfardus, Huda, Fischle, Wolfgang, Gapa, Liubov   +2 more
core   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

RNA‐related functions of BRCA2 in transcription, replication, and genome stability

FEBS Letters, EarlyView.
Beyond its role in homologous recombination, BRCA2 regulates R‐loop homeostasis, DNA–RNA hybrid resolution, RNA polymerase II dynamics, and transcriptional activation. Disruption of any of these functions can contribute to genome instability, inflammatory signaling, and tumorigenesis. BRCA2 is a central tumor suppressor protein best known for its roles
Lucia Alvaro‐Aranda, Sreerama Chaitanya Sridhara, Aura Carreira   +2 more
wiley   +1 more source