Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction [PDF]
NucleusAberrant nuclear morphology is a hallmark of human disease and causes nuclear dysfunction. Perturbed nuclear mechanics via reduced heterochromatin weakens the nucleus resulting in nuclear blebbing and rupture.
Gianna Manning +4 more
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Acute irradiation induces a senescence-like chromatin structure in mammalian oocytes [PDF]
Communications Biology, 2023 The mechanisms leading to changes in mesoscale chromatin organization during cellular aging are unknown. Here, we used transcriptional activator-like effectors, RNA-seq and superresolution analysis to determine the effects of genotoxic stress on oocyte ...
Claudia Baumann +9 more
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EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis [PDF]
Advanced ScienceEukaryotic translation initiation factor 5A (eIF5A) facilitates protein synthesis and impacts diverse biological processes, yet its role in transcriptional regulation is poorly understood.
Yuling Cai +15 more
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PLoS Genetics, 2021 Pericentromeric heterochromatin (PCH), the constitutive heterochromatin of pericentromeric regions, plays crucial roles in various cellular events, such as cell division and DNA replication.
Yota Hagihara +4 more
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L-2-hydroxyglutarate regulates centromere and heterochromatin conformation in the male germline. [PDF]
PLoS GeneticsGerm cell differentiation in the male testis involves extensive phenotypic, transcriptional, and epigenetic modifications, which are essential for producing functional spermatozoa.
Nina Mayorek +18 more
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Mouse chromocenters DNA content: sequencing and in silico analysis [PDF]
BMC Genomics, 2018 Background Chromocenters are defined as a punctate condensed blocks of chromatin in the interphase cell nuclei of certain cell types with unknown biological significance.
Dmitrii I. Ostromyshenskii +3 more
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SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state [PDF]
eLifeChromocenters are established after the 2-cell (2C) stage during mouse embryonic development, but the factors that mediate chromocenter formation remain largely unknown.
Ruben Sebastian-Perez +16 more
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NODeJ: an ImageJ plugin for 3D segmentation of nuclear objects [PDF]
BMC Bioinformatics, 2022 Background The three-dimensional nuclear arrangement of chromatin impacts many cellular processes operating at the DNA level in animal and plant systems.
Tristan Dubos +8 more
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Mechanisms governing the accessibility of DNA damage proteins to constitutive heterochromatin [PDF]
Frontiers in Genetics, 2022 Chromatin is thought to regulate the accessibility of the underlying DNA sequence to machinery that transcribes and repairs the DNA. Heterochromatin is chromatin that maintains a sufficiently high density of DNA packing to be visible by light microscopy ...
Anastasia Roemer +4 more
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Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression. [PDF]
PLoS BiologyNoncoding satellite DNA repeats are abundant at the pericentromeric heterochromatin of eukaryotic chromosomes. During interphase, sequence-specific DNA-binding proteins cluster these repeats from multiple chromosomes into nuclear foci known as ...
Ankita Chavan +12 more
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