Acute irradiation induces a senescence-like chromatin structure in mammalian oocytes [PDF]
Communications Biology, 2023 The mechanisms leading to changes in mesoscale chromatin organization during cellular aging are unknown. Here, we used transcriptional activator-like effectors, RNA-seq and superresolution analysis to determine the effects of genotoxic stress on oocyte ...
Claudia Baumann +9 more
doaj +2 more sources
The modular mechanism of chromocenter formation in Drosophila
eLife, 2019 A central principle underlying the ubiquity and abundance of pericentromeric satellite DNA repeats in eukaryotes has remained poorly understood. Previously we proposed that the interchromosomal clustering of satellite DNAs into nuclear structures known ...
Madhav Jagannathan +2 more
doaj +3 more sources
Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction [PDF]
NucleusAberrant nuclear morphology is a hallmark of human disease and causes nuclear dysfunction. Perturbed nuclear mechanics via reduced heterochromatin weakens the nucleus resulting in nuclear blebbing and rupture.
Gianna Manning +4 more
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L-2-hydroxyglutarate regulates centromere and heterochromatin conformation in the male germline. [PDF]
PLoS GeneticsGerm cell differentiation in the male testis involves extensive phenotypic, transcriptional, and epigenetic modifications, which are essential for producing functional spermatozoa.
Nina Mayorek +18 more
doaj +2 more sources
SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state [PDF]
eLifeChromocenters are established after the 2-cell (2C) stage during mouse embryonic development, but the factors that mediate chromocenter formation remain largely unknown.
Ruben Sebastian-Perez +16 more
doaj +2 more sources
Mechanisms governing the accessibility of DNA damage proteins to constitutive heterochromatin [PDF]
Frontiers in Genetics, 2022 Chromatin is thought to regulate the accessibility of the underlying DNA sequence to machinery that transcribes and repairs the DNA. Heterochromatin is chromatin that maintains a sufficiently high density of DNA packing to be visible by light microscopy ...
Anastasia Roemer +4 more
doaj +2 more sources
NODeJ: an ImageJ plugin for 3D segmentation of nuclear objects [PDF]
BMC Bioinformatics, 2022 Background The three-dimensional nuclear arrangement of chromatin impacts many cellular processes operating at the DNA level in animal and plant systems.
Tristan Dubos +8 more
doaj +2 more sources
Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression. [PDF]
PLoS BiologyNoncoding satellite DNA repeats are abundant at the pericentromeric heterochromatin of eukaryotic chromosomes. During interphase, sequence-specific DNA-binding proteins cluster these repeats from multiple chromosomes into nuclear foci known as ...
Ankita Chavan +12 more
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Mouse chromocenters DNA content: sequencing and in silico analysis [PDF]
BMC Genomics, 2018 Background Chromocenters are defined as a punctate condensed blocks of chromatin in the interphase cell nuclei of certain cell types with unknown biological significance.
Dmitrii I. Ostromyshenskii +3 more
doaj +3 more sources
A genetic basis for a postmeiotic X versus Y chromosome intragenomic conflict in the mouse. [PDF]
, 2012 Intragenomic conflicts arise when a genetic element favours its own transmission to the detriment of others. Conflicts over sex chromosome transmission are expected to have influenced genome structure, gene regulation, and speciation.
Affara, Nabeel A. +5 more
core +13 more sources