Results 221 to 230 of about 92,482 (260)
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Non-histone proteins and the structure of metaphase chromosomes
Experimental Cell Research, 1978Abstract Differential intensity of fluorescence corresponding to the banding patterns found in single metaphases can be obtained with isolated Chinese hamster chromosomes using the fluorochrome Hoechst 33258. Removal of histones from the chromosomes with 0.2 N HCl causes an approx.
A.T. Bankier +2 more
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The Primary Structure of a Non-histone Chromosomal Protein
European Journal of Biochemistry, 1977The primary structure of the calf thymus non-histone chromosomal protein HMG-17 has been determined. The sequence was determined mainly from data provided by the peptides obtained by cleavage with staphylococcal protease. Additional information was obtained from peptides produced by cleavage with trypsin and alpha-protease (from Crotalus atrox venom ...
John Walker +2 more
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Chromosomal non histone proteins and nuclear protein kinases
Biochimie, 1974Summary Some properties of chromosomal non histone proteins (NHP) have been studied: The biosynthesis of several NHP is modified in regenerating liver after hepatectomy, it is completely disturbed in Zajdela hepatoma cells. In cultured fibroblasts (coll. with Dr. Y.
Jacques Kruh +4 more
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A retropseudogene for non-histone chromosomal protein HMG-1
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1993Southern analysis of the human genome revealed that there are several sequences with homology to the nonhistone chromosomal protein HMG-1. The majority of the HMG-1 sequences are intronless as suggested from the polymerase chain reaction of HeLa DNA. Sequencing of a clone from a human placenta genomic library revealed that the clone was intronless and ...
Gordon H. Dixon, Michal Štros
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Chromosome replication and synthesis of non-histone proteins in giant polytene chromosomes
Chromosoma, 1968Synthesis of chromosomal proteins was studied by means of autoradiography in giant polytene chromosomes of Chironomus thummi. Incorporation of tryptophane-H3 into non-histone proteins of the chromosome does not increase during DNA synthesis. Grain count data reveal that chromosomes of cells which are actively replicating DNA do not differ from non ...
M. Donald Cave, M. Donald Cave
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Distribution and function of non-histone proteins in human chromosomes
Proceedings, annual meeting, Electron Microscopy Society of America, 1992The role of non-histone proteins in the structure and movements of mitotic chromosomes remains poorly understood. We describe here experiments aimed at characterization of the distribution of two very different classes of these proteins. The first is composed of integral components of the centromere (or primary constriction).
W.C. Earnshaw, C.A. Cooke
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Tissue specificity of tightly bound non-histone chromosomal proteins
Cell Differentiation, 1980Non-histone chromosomal proteins with high affinity for DNA were isolated from three different rat tissues (liver, spleen and kidney) and were analyzed by SDS-polyacrylamide gel electrophoresis. Each tissue showed a characteristic pattern of missing protein bands and in addition contained at least one unique band.
M. Yaneva, Roumen Tsanev, B. Beltchev
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Non-histone protein associated with centromeric heterochromatin in the mouse chromosome
Experimental Cell Research, 1977Abstract Distribution of protein in cytological preparations of the mouse metaphase chromosome was studied using protein-specific fluorochroming with dansyl chloride. Dansyl-fluorescence was relatively uniform along the axis of untreated chromosomes.
S. Matsukuma, T. Utakoji
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The partial amino acid sequence of a non-histone chromosomal protein
Biochemical and Biophysical Research Communications, 1976Abstract The amino acid sequence of the first thirty nine residues of the nonhistone chromosomal protein HMG-17 has been determined. Results presented here give a molecular weight of 11,000 for the protein. Some interesting sequence homology with the trout specific histone, histone-T, is noted.
John M. Walker +3 more
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Purification of a low molecular weight non-histone chromosomal protein
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1980A non-histone chromosomal proteins was extracted from rat liver chromatin with 0.35 M NaCl and purified more than 2758 times to near homogeneity by hydroxyapatite, gel filtration, and phosphocellulose chromatography. The final fraction was greater than 95% pure as judged by non-denaturing gel electrophoresis.
Frederick F. Becker, Richard A. Montagna
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