Results 61 to 70 of about 1,593,998 (268)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Are the processes of DNA replication and DNA repair reading a common structural chromatin unit?

Nucleus, 2020
Decades of investigation on genomic DNA have brought us deeper insights into its organization within the nucleus and its metabolic mechanisms. This was fueled by the parallel development of experimental techniques and has stimulated model building to ...
Stefania Mamberti, M. Cristina Cardoso
doaj   +1 more source

Every gene everywhere all at once: High-precision measurement of 3D chromosome architecture with single-cell Hi-C

Frontiers in Molecular Biosciences, 2022
The three-dimensional (3D) structure of chromosomes influences essential biological processes such as gene expression, genome replication, and DNA damage repair and has been implicated in many developmental and degenerative diseases.
Yi Chi, Yi Chi, Jenny Shi, Jenny Shi, Jenny Shi, Dong Xing, Dong Xing, Longzhi Tan, Longzhi Tan   +8 more
doaj   +1 more source

Clinical Impact of MGMT Promoter Methylation in IDH‐Mutant Gliomas: Influence of Threshold Selection and Clinical Confounding

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background The clinical relevance of MGMT promoter methylation in IDH‐mutant gliomas remains controversial in the era of molecular classification. We aimed to systematically evaluate its clinical relevance by integrating quantitative assessment, cutoff exploration, and adjustment for clinical confounding.
Haihui Jiang, Linxi Cao, Xiaodong Chen, Siqi Tong, Qinghui Zhu, Jun Yang, Song Lin, Yong Cui   +7 more
wiley   +1 more source

3PD: Rapid design of optimal primers for chromosome conformation capture assays

BMC Genomics, 2009
Background Higher eukaryotes control the expression of their genes by mechanisms that we are just beginning to understand. A complex layer of control is the dynamic spatial organization of the nucleus.
Dieterich Christoph, Fröhler Sebastian
doaj   +1 more source

Chromosome conformation capture technologies as tools to detect structural variations and their repercussion in chromatin 3D configuration

Frontiers in Cell and Developmental Biology, 2023
3D genome organization regulates gene expression in different physiological and pathological contexts. Characterization of chromatin structure at different scales has provided information about how the genome organizes in the nuclear space, from ...
Aura Stephenson-Gussinye, Mayra Furlan-Magaril   +1 more
doaj   +1 more source

3C-Based Chromatin Interaction Analyses

, 2018
This introduction presents a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function-chromosome conformation capture (3C).
Dekker, Job, Kim, Tae Hoon
core   +1 more source

Microfluidic Engineering of Microgels: A Review of Emerging Technologies, Materials, Fabrication Strategies, and Applications

Advanced Healthcare Materials, EarlyView.
Recent advances in microfluidic technologies for microgel fabrication are critically reviewed, highlighting the capabilities and limitations of major microfluidic platforms and discussing how platform design, material selection, and crosslinking strategies influence microgel architecture and functionality. Current challenges and future opportunities in
Afshin Abrishamkar, Laura Neely, Cynthia Pham, Nada Ibrahim, Alexis Larios‐Cervantes, Ben A. Kertesz, Todd Hoare   +6 more
wiley   +1 more source

Measuring Blood Plasma Glutamine Levels Using a Nanopore‐Based Sensor

Advanced Healthcare Materials, EarlyView.
Glutamine (GLN) is a biomarker useful for predicting toxic ammonia accumulation. Here, we used a biosensor based on the Cytolysin A (ClyA) nanopore coupled with the GLN‐specific protein substrate‐binding domain 2 (SBD2) to demonstrate real‐time measurement of GLN in blood plasma, highlighting the potential for future point‐of‐care applications ...
Sofia B. Relvas, Iris M. Kormelink, Sandra Michel‐Souzy, Florian L. R. Lucas, Nieck J. van der Heide, Hans R. Waterham, Ramon Versteeg, Judith Jans, Loes I. Segerink, Carsten Wloka   +9 more
wiley   +1 more source

Disruption of c-MYC Binding and Chromosomal Looping Involving Genetic Variants Associated With Ankylosing Spondylitis Upstream of the RUNX3 Promoter

Frontiers in Genetics, 2022
Background: Ankylosing Spondylitis (AS) is a common form of inflammatory spinal arthritis with a complex aetiology and high heritability, involving more than 100 genetic associations.
Carla J. Cohen, Carla J. Cohen, Connor Davidson, Connor Davidson, Connor Davidson, Carlo Selmi, Paul Bowness, Paul Bowness, Julian C. Knight, B. Paul Wordsworth, B. Paul Wordsworth, Matteo Vecellio, Matteo Vecellio, Matteo Vecellio   +13 more
doaj   +1 more source