Results 71 to 80 of about 1,593,998 (268)

Chromosome conformation capture in primary human cells

, 2016
3D organization of the genome, its structural and regulatory function of cell identity, is acquiring prominent features in epigenetics studies; more efforts have been done to develop techniques that allow studying nuclear structure.
Bodega B., Cortesi A.
core   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Computational methods for analyzing genome-wide chromosome conformation capture data

, 2018
In all organisms, chromatin is packed to fulfil structural constraints and functional requirements. The hierarchical model of chromatin organization in the 3D nuclear space encompasses different topologies at diverse scale lengths, with chromosomes ...
Nicoletti, Chiara, Silvio Bicciato, Forcato, Mattia, Chiara Nicoletti, Bicciato, Silvio, Mattia Forcato   +5 more
core   +1 more source

A Rare De Novo Missense Mutation in IFT122 Confers a Genetic Susceptibility Factor of Idiopathic Pediatric Uveitis Via Trio‐based Whole‐Exome Sequencing

Advanced Science, EarlyView.
A rare de novo IFT122‐A773E variant is identified in idiopathic pediatric uveitis and shown to exacerbate retinal inflammation and barrier dysfunction. Mechanistically, the variant enhances IFT43 interaction, elevates calcium signaling, and activates the MEK/ERK/FRA1 axis, revealing a previously unrecognized cilia‐associated pathway that may increase ...
Qian Zhou, Jiaxing Huang, Yilin Wang, Xingran Li, Xianyang Liu, Lingyu Dai, Hongshun Li, Qingfeng Wang, Jiangyi Liu, Guannan Su, Wanyun Zhang, Yang Deng, Qingfeng Cao, Yujie Lai, Xiang Luo, Changwei Huang, Ling Chen, Shengping Hou, Peizeng Yang   +18 more
wiley   +1 more source

3C and 3C-based techniques: the powerful tools for spatial genome organization deciphering

Molecular Cytogenetics, 2018
It is well known that the chromosomes are organized in the nucleus and this spatial arrangement of genome play a crucial role in gene regulation and genome stability. Different techniques have been developed and applied to uncover the intrinsic mechanism
Jinlei Han, Zhiliang Zhang, Kai Wang
doaj   +1 more source

4See: A Flexible Browser to Explore 4C Data

Frontiers in Genetics, 2020
It is established that transcription of many metazoan genes is regulated by distal regulatory sequences beyond the promoter. Enhancers have been identified at up to megabase distances from their regulated genes, and/or proximal to or within the introns ...
Yousra Ben Zouari, Yousra Ben Zouari, Yousra Ben Zouari, Yousra Ben Zouari, Angeliki Platania, Angeliki Platania, Angeliki Platania, Angeliki Platania, Anne M. Molitor, Anne M. Molitor, Anne M. Molitor, Anne M. Molitor, Tom Sexton, Tom Sexton, Tom Sexton, Tom Sexton   +15 more
doaj   +1 more source

Detecting long-range chromatin interactions using the chromosome conformation capture sequencing (4C-seq) method

, 2011
Eukaryotic transcription is tightly regulated by transcriptional regulatory elements, even though these elements may be located far away from their target genes. It is now widely recognized that these regulatory elements can be brought in close proximity
Reymond, Alexandre, Leleu, Marion, Rougemont, J., Marion Leleu, Gheldof, N., Noordermeer, Daan, Reymond, A., Jacques Rougemont, Nele Gheldof, Noordermeer, D., Gheldof, Nele, Rougemont, Jacques, Daan Noordermeer, Alexandre Reymond, Leleu, M., Leleu M, Gheldof N, Rougemont J, Reymond A, Noordermeer D   +19 more
core   +1 more source

Data_Sheet_1_Investigation of the Basic Steps in the Chromosome Conformation Capture Procedure.pdf

, 2021
A constellation of chromosome conformation capture methods (С-methods) are an important tool for biochemical analysis of the spatial interactions between DNA regions that are separated in the primary sequence.
Yulii V. Shidlovskii (3838981), Anna E. Pravednikova (11454502), Oleg V. Bylino (11454496), Airat N. Ibragimov (11454499)   +3 more
core   +1 more source

Regulating Cells Fate and Function to Facilitate Bone Regeneration via Designing Programmable Bio‐Interactive Materials

Advanced Science, EarlyView.
This article aims to elucidate the biological mechanisms of bone repair and the evolution of material design, highlighting key cellular and molecular processes. It further proposes strategies and prospects for programmable bio‐interactive materials, which enable precisely guided bone tissue regeneration by dynamically regulating cell behavior and the ...
Qingrui Fan, Fuming Cao, Junqiang Mao, Pengbin Yin, Licheng Zhang, Jianjun Wang, Peifu Tang   +6 more
wiley   +1 more source

The Landscape of Long Non‐Coding RNAs Provides Insights Into the Domestication and Improvement of Pear Fruit

Advanced Science, EarlyView.
This study reveals that long non‐coding RNAs represent important regulatory components underlying pear domestication and improvement. Integrative multi‐omics analyses identify selected lncRNAs associated with fruit traits, while functional validation uncovers a lignin‐related lncRNA, lncRNA‐pys, that promotes lignin accumulation.
Bobo Song, Peilin Han, Jiaming Li, Xiaolong Li, Xiang Zhang, Jing Fan, Dongliang Liu, Jianfa Cai, Lingchao Zhang, Jun Wu   +9 more
wiley   +1 more source