Results 91 to 100 of about 1,593,998 (268)

3C-BASED METHODS FOR 3D GENOME ORGANIZATION ANALYSIS

Вавиловский журнал генетики и селекции, 2014
The 3D organization of an eukaryotic genome plays an important role in nuclear gene expression. Until recently, studies of this organization were limited by light microscopy and electron microscopy methods.
N. R. Battulin, V. S. Fishman, Yu. L. Orlov, A. G. Menzorov, D. A. Afonnikov, O. L. Serov   +5 more
doaj  

Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence.

PLoS Genetics, 2020
Telomeres have the ability to adopt a lariat conformation and hence, engage in long and short distance intra-chromosome interactions. Budding yeast telomeres were proposed to fold back into subtelomeric regions, but a robust assay to quantitatively ...
Tina Wagner, Lara Pérez-Martínez, René Schellhaas, Marta Barrientos-Moreno, Merve Öztürk, Félix Prado, Falk Butter, Brian Luke   +7 more
doaj   +1 more source

Can chromatin conformation technologies bring light into human molecular pathology? [PDF]

, 2015
Regulation of gene expression in eukaryotes involves many complex processes, in which chromatin structure plays an important role. In addition to the epigenetic effects, such as DNA methylation and phosphorylation or histone modifications, gene ...
Lewandowska, Marzena, Kubiak, Marta
core   +1 more source

Optimized Cas9‐Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders

Advanced Science, EarlyView.
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee, Chanju Jung, Minjeong Kim, Narae Kim, Gue‐Ho Hwang, Soon‐Tae Lee, Kon Chu, Sang Kun Lee, Han‐Joon Kim, Jong‐Hee Chae, Sangsu Bae, Jangsup Moon   +11 more
wiley   +1 more source

Crosslinking intensity modulates the reliability and sensitivity of chromatin conformation detection at different structural levels

Communications Biology
Formaldehyde (FA) is a chemical that facilitates crosslinking between DNA and proteins. It is widely used in various biochemical assays, such as chromosome conformation capture (3C) and Chromatin Immunoprecipitation (ChIP).
Bingxiang Xu, Xiaomeng Gao, Xiaoli Li, Feifei Li, Zhihua Zhang   +4 more
doaj   +1 more source

High-resolution circular chromosome conformation capture assay

, 2008
The pioneering chromosome conformation capture (3C) method provides the opportunity to study chromosomal folding in the nucleus. It is based on formaldehyde cross-linking of living cells followed by enzyme digestion, intramolecular ligation and ...
Gondor, A, Ohlsson, Rolf,, Rougier, C, Anita Göndör, Carole Rougier, Rougier, Carole,, Rolf Ohlsson, Göndör, Anita,, Ohlsson, R   +8 more
core   +1 more source

Glucose and Glutamine Deprivation Promotes Breast Cancer Lung Metastasis via BRD4‐Dependent Enhancer Activation

Advanced Science, EarlyView.
To model a nutrient‐restricted tumor microenvironment, breast cancer cells were adapted to low glucose and glutamine levels. In the adapted cells, BRD4‐driven enhancer activation increases pro‐invasive EDN1 expression, facilitated by ATF3/c‐JUN and reinforced by enhancer RNA.
Poshan Yugal Bhattarai, Arathy Vasukutty, Yujin Na, Sung‐Chul Lim, Hong Seok Choi   +4 more
wiley   +1 more source

Cancer‐Associated Fibroblasts Promote Glucose Metabolic Reprogramming and Progression of Triple‐Negative Breast Cancer via Exosomal circFAD104‐Mediated Intercellular Communication

Advanced Science, EarlyView.
CAF‐derived exosomes deliver circFAD104 into TNBC cells, where it acts as a molecular scaffold that bridges the E3 ligase MARCHF8 and PGM1, promoting MARCHF8‐mediated K48‐linked ubiquitination and proteasomal degradation of PGM1. Loss of PGM1 redirects glucose‐phosphate flux from glycogen synthesis toward glycolysis, thereby driving stemness, EMT, and ...
Lei Wang, Xiaoyan Li, Yiran Liang, Yifei Wang, Yuhan Jin, Jianing Wang, Dan Luo, Yuxia Yang, Jingze Yang, Yaming Li, Tong Chen, Dianwen Han, Zekun Wang, Wenjing Zhao, Bing Chen, Lijuan Wang, Qifeng Yang   +16 more
wiley   +1 more source

Technical Review: A Hitchhiker’s Guide to Chromosome Conformation Capture

, 2017
International audienceThe introduction of chromosome conformation capture (3C) technologies boosted the field of 3D-genome research and significantly enhanced the available toolset to study chromosomal architecture.
Giacomo Cavalli, Stefan Grob, Grob, Stefan, Cavalli, Giacomo   +3 more
core   +1 more source

MethyAnno: An Interpretable Automated Annotation Method Leveraging Multi‐Scale Information and Metric Learning Framework for scDNAm Data

Advanced Science, EarlyView.
MethyAnno enables robust and interpretable annotation of single‐cell DNA methylation data by integrating multi‐scale epigenetic information, bidirectional cross‐attention, and prototype‐based metric learning. The framework resolves rare and novel cell types across datasets while revealing cell‐type‐specific epigenetic signatures associated with disease
Yuhang Jia, Siyu Li, Songming Tang, Keju Gu, Shengquan Chen   +4 more
wiley   +1 more source