Results 31 to 40 of about 210,847 (212)

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Combination of AID2 and BromoTag expands the utility of degron-based protein knockdowns

EMBO Reports
Acute protein knockdown is a powerful approach to dissecting protein function in dynamic cellular processes. We previously reported an improved auxin-inducible degron system, AID2, but recently noted that its ability to induce degradation of some ...
Yuki Hatoyama, Moutushi Islam, Adam G Bond, Ken-ichiro Hayashi, Alessio Ciulli, Masato T Kanemaki   +5 more
doaj   +1 more source

SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription

Cell Reports, 2017
The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that ...
Ngat T. Tran, Michael T. Laub, Tung B.K. Le   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like Tail

mBio, 2017
In bacteria, chromosomal DNA must be efficiently compacted to fit inside the small cell compartment while remaining available for the proteins involved in replication, segregation, and transcription.
Joanna Hołówka, Damian Trojanowski, Katarzyna Ginda, Bartosz Wojtaś, Bartłomiej Gielniewski, Dagmara Jakimowicz, Jolanta Zakrzewska-Czerwińska   +6 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress

Nature Communications
RIF1 is a multifunctional protein that regulates DNA replication and repair. RIF1-deficient cells are hypersensitive to DNA replication stress. Of the two alternatively spliced RIF1 isoforms, called RIF1-Short and RIF1-Long, the RIF1-Long isoform is more
Qianqian Dong, Matthew Day, Yuichiro Saito, Emma Parker, Lotte P. Watts, Masato T. Kanemaki, Antony W. Oliver, Laurence H. Pearl, Shin-ichiro Hiraga, Anne D. Donaldson   +9 more
doaj   +1 more source

The RIF1-long splice variant promotes G1 phase 53BP1 nuclear bodies to protect against replication stress

eLife, 2020
Human cells lacking RIF1 are highly sensitive to replication inhibitors, but the reasons for this sensitivity have been enigmatic. Here, we show that RIF1 must be present both during replication stress and in the ensuing recovery period to promote cell ...
Lotte P Watts, Toyoaki Natsume, Yuichiro Saito, Javier Garzon, Qianqian Dong, Lora Boteva, Nick Gilbert, Masato T Kanemaki, Shin-ichiro Hiraga, Anne D Donaldson   +9 more
doaj   +1 more source

Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes

iScience, 2021
Summary: The proximity pattern and radial distribution of chromosome territories within spherical nuclei are random and non-random, respectively. Whether this distribution pattern is conserved in the partitioned or lobed nuclei of polymorphonuclear cells
Christine R. Keenan, Michael J. Mlodzianoski, Hannah D. Coughlan, Naiara G. Bediaga, Gaetano Naselli, Erin C. Lucas, Qike Wang, Carolyn A. de Graaf, Douglas J. Hilton, Leonard C. Harrison, Gordon K. Smyth, Kelly L. Rogers, Thomas Boudier, Rhys S. Allan, Timothy M. Johanson   +14 more
doaj   +1 more source