Results 11 to 20 of about 210,847 (212)

Constructing Auxin-Inducible Degron Mutants Using an All-in-One Vector

Pharmaceuticals, 2020
Conditional degron-based methods are powerful for studying protein function because a degron-fused protein can be rapidly and efficiently depleted by adding a defined ligand.
Aisha Yesbolatova, Yuichiro Saito, Masato T. Kanemaki   +2 more
doaj   +1 more source

Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA

Cell Reports, 2016
Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood.
Anita Minnen, Frank Bürmann, Larissa Wilhelm, Anna Anchimiuk, Marie-Laure Diebold-Durand, Stephan Gruber   +5 more
doaj   +1 more source

SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis

eLife, 2015
Smc–ScpAB forms elongated, annular structures that promote chromosome segregation, presumably by compacting and resolving sister DNA molecules. The mechanistic basis for its action, however, is only poorly understood. Here, we have established a physical
Larissa Wilhelm, Frank Bürmann, Anita Minnen, Ho-Chul Shin, Christopher P Toseland, Byung-Ha Oh, Stephan Gruber   +6 more
doaj   +1 more source

Chromosome Architecture and Genome Organization. [PDF]

PLoS ONE, 2015
How the same DNA sequences can function in the three-dimensional architecture of interphase nucleus, fold in the very compact structure of metaphase chromosomes and go precisely back to the original interphase architecture in the following cell cycle ...
Giorgio Bernardi
doaj   +1 more source

Bacterial chromosome segregation by the ParABS system [PDF]

Open Biology, 2020
Proper chromosome segregation during cell division is essential in all domains of life. In the majority of bacterial species, faithful chromosome segregation is mediated by the tripartite ParABS system, consisting of an ATPase protein ParA, a CTPase and ...
Adam S. B. Jalal, Tung B. K. Le
doaj   +1 more source

Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci

Nature Communications, 2022
Trans-chromosomic (Tc) mice have helped the development of therapeutic antibodies, but chromosome instability limits its application. Here the authors develop a new line of Tc mice with full human Ig heavy and kappa loci integrated into the mouse ...
Hiroyuki Satofuka, Satoshi Abe, Takashi Moriwaki, Akane Okada, Kanako Kazuki, Hiroshi Tanaka, Kyotaro Yamazaki, Genki Hichiwa, Kayoko Morimoto, Haruka Takayama, Yuji Nakayama, Shinya Hatano, Yutaro Yada, Yasufumi Murakami, Yoshihiro Baba, Mitsuo Oshimura, Kazuma Tomizuka, Yasuhiro Kazuki   +17 more
doaj   +1 more source

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

Nature Communications, 2020
Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.
Aisha Yesbolatova, Yuichiro Saito, Naomi Kitamoto, Hatsune Makino-Itou, Rieko Ajima, Risako Nakano, Hirofumi Nakaoka, Kosuke Fukui, Kanae Gamo, Yusuke Tominari, Haruki Takeuchi, Yumiko Saga, Ken-ichiro Hayashi, Masato T. Kanemaki   +13 more
doaj   +1 more source

Novel Divisome-Associated Protein Spatially Coupling the Z-Ring with the Chromosomal Replication Terminus in Caulobacter crescentus

mBio, 2020
Cell division requires proper spatial coordination with the chromosome, which undergoes dynamic changes during chromosome replication and segregation. FtsZ is a bacterial cytoskeletal protein that assembles into the Z-ring, providing a platform to build ...
Shogo Ozaki, Urs Jenal, Tsutomu Katayama
doaj   +1 more source

Genome Copy Number Quantification Revealed That the Ethanologenic Alpha-Proteobacterium Zymomonas mobilis Is Polyploid

Frontiers in Microbiology, 2021
The alpha-proteobacterium Zymomonas mobilis is a promising biofuel producer, based on its native metabolism that efficiently converts sugars to ethanol. Therefore, it has a high potential for industrial-scale biofuel production.
Katsuya Fuchino, Katsuya Fuchino, Daniel Wasser, Jörg Soppa   +3 more
doaj   +1 more source

The complete and fully assembled genome sequence of Aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other Aeromonas species: investigation of the mobilome in environmental and pathogenic strains

BMC Genomics, 2018
Background Due to the predominant usage of short-read sequencing to date, most bacterial genome sequences reported in the last years remain at the draft level.
Friedhelm Pfeiffer, Maria-Antonia Zamora-Lagos, Martin Blettinger, Assa Yeroslaviz, Andreas Dahl, Stephan Gruber, Bianca H. Habermann   +6 more
doaj   +1 more source