Results 61 to 70 of about 923,115 (353)

Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments

Nature Communications, 2022
Tension stabilizes properly attached microtubules to kinetochores during chromosome segregation, and lack of tension leads to release. Here the authors show that tension directly suppresses Aurora B kinase mediated destabilization of reconstituted ...
Anna K. de Regt, Cordell J. Clark, Charles L. Asbury, Sue Biggins   +3 more
doaj   +1 more source

MUS81 nuclease activity is essential for replication stress tolerance and chromosome segregation in BRCA2-deficient cells

Nature Communications, 2017
Failure to restart replication forks stalled at genomic regions that are difficult to replicate or contain endogenous DNA lesions is a hallmark of BRCA2 deficiency.
Xianning Lai, R. Broderick, V. Bergoglio, J. Zimmer, Sophie Badie, W. Niedzwiedz, J. Hoffmann, M. Tarsounas   +7 more
semanticscholar   +1 more source

MYC Binding Near Transcriptional End Sites Regulates Basal Gene Expression, Read‐Through Transcription, and Intragenic Contacts

Advanced Science, EarlyView.
MYC is a transcription factor (TF) that binds DNA near transcriptional start sites (TSSs) and within enhancer elements. Here, unappreciated sites of MYC binding in the vicinity of transcriptional end sites (TESs) of many genes in multiple cell types in association with numerous other TFs are described previously.
Huabo Wang, Bingwei Ma, Taylor Stevens, Jessica Knapp, Jie Lu, Edward V. Prochownik   +5 more
wiley   +1 more source

Unique Function of the Bacterial Chromosome Segregation Machinery in Apically Growing Streptomyces - Targeting the Chromosome to New Hyphal Tubes and its Anchorage at the Tips.

PLoS Genetics, 2016
The coordination of chromosome segregation with cell growth is fundamental to the proliferation of any organism. In most unicellular bacteria, chromosome segregation is strictly coordinated with cell division and involves ParA that moves the ParB ...
Agnieszka Kois-Ostrowska, Agnieszka Strzałka, Natalia Lipietta, Emma Tilley, Jolanta Zakrzewska-Czerwińska, Paul Herron, Dagmara Jakimowicz   +6 more
doaj   +1 more source

Chromosome segregation occurs by microtubule pushing in oocytes

Nature Communications, 2017
During cell division, spindle microtubules ensure an equal repartition of chromosomes between the two daughter cells. While the kinetochore-dependent mechanisms that drive mitotic chromosome segregation are well understood, in oocytes of most species ...
K. Laband, R. Le Borgne, F. Edwards, Marine Stefanutti, J. Canman, J. Verbavatz, J. Dumont   +6 more
semanticscholar   +1 more source

Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos

Advanced Science, EarlyView.
This study performs a comprehensive single‐cell transcriptome analysis of sheep embryos from embryonic (E) day 1 to E14 to elucidate the mechanism of early lineage specification and the pluripotency changes (naïve, formative, and primed) of epiblast.
Jinying Zhang, Runbo Li, Ruijie Luo, Qiang Zhang, Zimo Zhao, Haishen Xu, Minglei Zhi, Wenjie Jiang, Meng Wang, Xinze Chen, Zhiqiang Feng, Yingjie Wang, Yuhan Yang, Pengcheng He, Hanyue Su, Tianzhi Chen, Shunxin Wang, Yixuan Yao, Jinghui Yang, Fan Zhao, Tingting Li, Haitao Wang, Xiaosheng Zhang, Jinlong Zhang, Qiuyue Liu, Shuai Gao, Yuchang Yao, Jianyong Han, Suying Cao   +28 more
wiley   +1 more source

Chromosome Replication and Segregation in Bacteria

Annual Review of Genetics, 2012
In dividing cells, chromosome duplication once per generation must be coordinated with faithful segregation of newly replicated chromosomes and with cell growth and division. Many of the mechanistic details of bacterial replication elongation are well established. However, an understanding of the complexities of how replication initiation is controlled
Reyes-Lamothe, Rodrigo, Nicolas, Emilien, Sherratt, David J   +2 more
openaire   +3 more sources

Cell-Cycle Regulation of Dynamic Chromosome Association of the Condensin Complex

Cell Reports, 2018
Summary: Eukaryotic cells inherit their genomes in the form of chromosomes, which are formed from the compaction of interphase chromatin by the condensin complex. Condensin is a member of the structural maintenance of chromosomes (SMC) family of ATPases,
Rahul Thadani, Julia Kamenz, Sebastian Heeger, Sofía Muñoz, Frank Uhlmann   +4 more
doaj  

Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling

STAR Protocols, 2020
Summary: Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS).
Andrew W. Jones, Helen R. Flynn, Frank Uhlmann, Ambrosius P. Snijders, Sandra A. Touati   +4 more
doaj  

A time out for CENP-A

Molecular & Cellular Oncology, 2017
Proper chromosome segregation relies on a functional centromere–kinetochore interface. We showed that chromatin containing CENtromere Protein A (CENP-A) is essential for centromere assembly, but dispensable for chromosome segregation in the presence of ...
S. Hoffmann, D. Fachinetti
doaj   +1 more source