Results 61 to 70 of about 66,240 (247)

DistAMo: A web-based tool to characterize DNA-motif distribution on bacterial chromosomes

Frontiers in Microbiology, 2016
Short DNA motifs are involved in a multitude of functions such as for example chromosome segregation, DNA replication or mismatch repair. Distribution of such motifs is often not random and the specific chromosomal pattern relates to the respective motif
Patrick eSobetzko, Lukas eJelonek, Marc eStrickert, Wenxia eHan, Alexander eGoesmann, Torsten eWaldminghaus   +5 more
doaj   +1 more source

Chromosome‐Level Genome Assembly of the Leafcutter Bee Megachile rotundata Reveals Its Ecological Adaptation and Pollination Biology

Advanced Science, EarlyView.
The genome of Megachile rotundata, an extensively managed solitary pollination bee species, has been sequenced, covering 280.68 Mb and predicting 10 701 genes. The study reveals significant expansions of the Toll gene family and their abundant expression in diapause prepupae, highlighting enhanced immune responses during diapause. This genome serves as
Rangjun Shi, Pan Duan, Mengmeng Zhu, Rong Zhang, Zihua Zhao, Xin Nie, Hanhou He, Li Hou, Xianhui Wang   +8 more
wiley   +1 more source

FRUIT, a scar-free system for targeted chromosomal mutagenesis, epitope tagging, and promoter replacement in Escherichia coli and Salmonella enterica. [PDF]

PLoS ONE, 2012
Recombineering is a widely-used approach to delete genes, introduce insertions and point mutations, and introduce epitope tags into bacterial chromosomes. Many recombineering methods have been described, for a wide range of bacterial species.
Anne M Stringer, Navjot Singh, Anastasiya Yermakova, Brianna L Petrone, Jayaleka J Amarasinghe, Lucia Reyes-Diaz, Nicholas J Mantis, Joseph T Wade   +7 more
doaj   +1 more source

METTL14‐Induced M6A Methylation Increases G6pc Biosynthesis, Hepatic Glucose Production and Metabolic Disorders in Obesity

Advanced Science, Volume 12, Issue 22, June 12, 2025.
It is shown that obesity is associated with increases in hepatic METTL14 and m6A methylation of G6pc transcript. YTHDF1 and YTHDF3 bind to m6A‐marked G6pc mRNA to increase its synthesis. Hepatocyte‐specific deletion of Mettl14 decreases G6pc m6A methylation, G6pc biosynthesis, and G6pc‐mediated gluconeogenesis, alleviating glucose metabolic defects in ...
Qiantao Zheng, Xiao Zhong, Qianqian Kang, Zhiguo Zhang, Decheng Ren, Yong Liu, Liangyou Rui   +6 more
wiley   +1 more source

Selection Effects on the Positioning of Genes and Gene Structures from the Interplay of Replication and Transcription in Bacterial Genomes

Evolutionary Bioinformatics, 2007
Bacterial chromosomes are partly shaped by the functional requirements for efficient replication, which lead to strand bias as commonly characterized by the excess of guanines over cytosines in the leading strand.
Kazuharu Arakawa, Masaru Tomita
doaj  

Linear dicentric chromosomes in bacterial natural isolates reveal common constraints for replicon fusion

mBio
Multipartite bacterial genome organization can confer advantages, including coordinated gene regulation and faster genome replication, but is challenging to maintain.
Ram Sanath-Kumar, Arafat Rahman, Zhongqing Ren, Ian P. Reynolds, Lauren Augusta, Clay Fuqua, Alexandra J. Weisberg, Xindan Wang   +7 more
doaj   +1 more source

Production of Shotgun Libraries Using Random Amplification

BioTechniques, 2001
In the following report, thermal cycling coupled with random 10-mers as primers was used to construct randomly amplified shotgun libraries (RASLs). This approach allowed shotgun libraries to be constructed from nanogram quantities of input DNA.
F. Rohwer, V. Seguritan, D.H. Choi, A.M. Segall, F. Azam   +4 more
doaj   +1 more source

LncRNA‐MEG3 Regulates Muscle Mass and Metabolic Homeostasis by Facilitating SUZ12 Liquid–Liquid Phase Separation

Advanced Science, EarlyView.
This study has identified lncRNA‐MEG3 as a key regulator of muscle mass, promoting slow‐twitch muscle fibers and preventing muscle atrophy. By stabilizing the SUZ12/PRC2 complex through liquid–liquid phase separation (LLPS), lncRNA‐MEG3 influences mitochondrial function and lipid metabolism.
Yilong Yao, Chao Yan, Haibo Huang, Shilong Wang, Jiaying Li, Yun Chen, Xiaolu Qu, Qi Bao, Lingna Xu, Yuanyuan Zhang, Danyang Fan, Xia He, Yanwen Liu, Yongsheng Zhang, Yalan Yang, Zhonglin Tang   +15 more
wiley   +1 more source

Editorial for Special Issue “Bacterial Toxin-Antitoxin Systems”

Microorganisms
Toxin antitoxin systems (TAS) are widely distributed in bacterial chromosomes as well as on mobile genetic elements [...]
Muhammad Kamruzzaman
doaj   +1 more source

SMC4 Promotes Prostate Cancer Cell Proliferation and Metastasis via the Rheb/mTOR Pathway

Advanced Science, EarlyView.
Structural maintenance of chromosome protein 4 (SMC4) has been implicated in prostate cancer metastasis. Knockdown of SMC4 in RM1‐LM cells significantly attenuates proliferation, migration, and lung metastasis in vivo. Furthermore, SMC4 interacts with GLUT1 (Slc2a1), thereby enhancing glycolysis, ATP production, and activation of the Rheb/mTOR pathway.
Wei Zhang, Siyuan Qin, Xiaokang Li, Ming Chang, Tianzi Wei, Xin Huang, Haibo Tong, Xia Guo, Yi Lu, Jian Zhang   +9 more
wiley   +1 more source