Results 161 to 170 of about 5,041 (212)

Expression and characterization of camel chymosin in Pichia pastoris

Protein Expression and Purification, 2015
Chymosin efficiently coagulates milk and so is widely used in commercial cheese production. Traditional chymosin production requires the slaughter of a large numbers of unweaned calves. In the present study, a full-length camel prochymosin gene was synthesized and cloned into the pPIC9K vector, which was then inserted into the yeast strain, Pichia ...
Dehu Liu
exaly   +3 more sources

Generation and characterization of caprine chymosin in corn seed

Protein Expression and Purification, 2017
Chymosin is widely used in the dairy industry, and much is produced through recombinant DNA in organisms such as bacteria and tobacco. In this study, we used a new transgenic method to express caprine chymosin in corn seeds with lower cost and better storage capability.
Yun-Peng Wang
exaly   +3 more sources

Bovine chymosin

Biotechnology Advances, 1999
A.K Mohanty   +3 more
exaly   +2 more sources

Improved Production of Chymosin in Aspergillus by Expression as a Glucoamylase-Chymosin Fusion

Nature Biotechnology, 1990
We have extended the work on chymosin production in Aspergillus by constructing an expression vector in which the cDNA encoding bovine prochymosin B was fused in frame immediately following the codon for the last amino acid of the A. awamori glucoamylase (glaA) gene. Transformation of A.
M, Ward   +4 more
openaire   +2 more sources

The crystallography of calf rennin (chymosin)

Proceedings of the Royal Society of London. Series B. Biological Sciences, 1971
Abstract The growth of crystals of calf rennin (chymosin, † EC 3.4.4.3) and the control of nucleation to produce crystals of desired size, are described. Only one stable species, orthorhombic rennin I, has been found, but a metastable monoclinic species, rennin II, appeared on one occasion in a solution nearly saturated with glycine ...
C W, Bunn, P C, Moews, M E, Baumber
openaire   +2 more sources

Increased production of chymosin by glycosylation

Journal of Biotechnology, 2006
Filamentous fungi are well known in the industry as producers of large amounts of extracellular proteins. However, production levels of heterologous proteins are often disappointing low. In this paper it is shown that increasing glycosylation is a powerful strategy for increasing production levels of chymosin in filamentous fungi.
Hans J M, van den Brink   +4 more
openaire   +2 more sources

INFLUENCE of ULTRASOUND ON the PROPERTIES of CHYMOSIN and the ULTRASTRUCTURE of ABOMASUM DURING CHYMOSIN EXTRACTION

Journal of Food Processing and Preservation, 1991
The effects of ultrasound treatment on the properties of chymosin and the ultrastructure of abomasum during chymosin extraction were investigated. Ultrasound treatment at 36 W/cm2 for 80 min did not decrease chymosin activity and its proteolytic activity.
S. M. KIM, J. F. ZAYAS
openaire   +1 more source

Chymosin

2004
Chymosin
Foltmann, Bent, Szecsi, Pal B.
openaire   +1 more source

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