Results 261 to 270 of about 186,921 (280)

Succinylated and acetylated concanavalin A activate the classical complement pathway

Biochemical and Biophysical Research Communications, 1981
Abstract Succinylated and acetylated concanavalin A, but not the native lectin, lyse sheep erythrocytes in the presence of guinea pig complement. The effect appears to be specific since succinylated wheat germ agglutinin is inactive and hemolysis is inhibited selectively by α-D-methylglucopyranoside. Hemolytic activity is enhanced by preincubation of
J J, Langone, R, Ejzemberg
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Tachyplesin Activates the Classic Complement Pathway to Kill Tumor Cells

Cancer Research, 2005
Abstract Tachyplesin is a small, cationic peptide that possesses antitumor properties. However, little is known about its action mechanism. We used phage display to identify a protein that interacted with tachyplesin and isolated a sequence corresponding to the collagen-like domain of C1q, a key component in the complement pathway. Their
Chen, J. G., Xu, X. N., Underhfll, C. B., Yang, S. M., Wang, L. P., Chen, Y. X., Hong, S. G., Creswell, K., Zhang, L. R.   +8 more
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Adiponectin binds C1q and activates the classical pathway of complement

Biochemical and Biophysical Research Communications, 2008
The adipose-specific protein adiponectin binds to a number of target molecules, including damaged endothelium and the surface of apoptotic cells. However, the significance of this binding remains unclear. This study demonstrates the binding of purified C1q to recombinant adiponectin under physiological conditions, and the dependence of this upon Ca(++)
Philip W, Peake, Yvonne, Shen, Alexandra, Walther, John A, Charlesworth   +3 more
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An immunofluorescence assay for complement activation by the classical pathway

Journal of Immunological Methods, 1981
The functional integrity of classical complement pathway components was determined by an immunofluorescence (IFL) assay based on the capacity of cytoskeletal intermediate filaments (IMF) to bind C1q and to activate the complement pathway. The assay uses IMF-rich capillary endothelium of human term placentae as complement-activating substrate.
E, Linder, M, Rhen, S, Meri
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New insights into the molecular mechanisms of classical complement activation

Molecular Immunology, 2010
C1q, the initiator of the classical complement cascade, is a versatile molecule with numerous ligands and variety of functions. Recent mutagenesis, epitope mapping and structural data brought novel understanding of the molecular mechanisms of C1q binding to target molecules, and subsequent C1 activation.
Mihaela, Kojouharova, Kenneth, Reid, Mihaela, Gadjeva   +2 more
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Monomeric C‐reactive protein modulates classic complement activation on necrotic cells

The FASEB Journal, 2011
ABSTRACT The acute‐phase protein C‐reactive protein (CRP) recruits C1q to the surface of damaged cells and thereby initiates complement activation. However, CRP also recruits complement inhibitors, such as C4b‐binding protein (C4bp) and factor H, which both block complement progression at the level of C3 and ...
Mihlan, M., Blom, A.M., Kupreishvili, K., Lauer, N., Stelzner, K., Bergstrom, F., Niessen, H.W.M., Zipfel, P.F.   +7 more
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The Classical Complement Pathway: Activation and Regulation of the First Complement Component

1985
Publisher Summary This chapter focuses on the activation and regulation of the first complement component. . The chapter outlines the history of the classical pathway of the complement system. Clq and C1 are bound and activated by immune complexes or aggregates containing IgG or IgM but not by those containing IgA, IgD, or IgE . Among IgG subclasses,
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Alternative complement pathway activation by C4b deposited during classical pathway activation.

The Journal of Immunology, 1986
Abstract Sheep erythrocytes (E) sensitized with anti-E antibody (A) were reacted with guinea pig C1 (C1gp) and human C4 (C4hu) or guinea pig C4 (C4gp) to prepare EAC1, 4b. Treatment of the EAC1, 4b with a buffer containing EDTA removes C1rgp and C1sgp, resulting in the formation of EAC4b. EAC4b prepared in this way were found to be lysed
M, Matsushita, H, Okada
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Activation of the Classic and Alternate Complement Pathways by Endotoxin

The Journal of Immunology, 1974
Abstract The ability of bacterial endotoxin (LPS) to activate the complement system was studied in guinea pig serum (GPS). In serum chelated with ethyleneglycol tetraacetic acid (EGTA) 10 mM, which permits alternate complement pathway activation but inhibits classic complement pathway activation, lysis of LPS-coated sheep erythrocytes (E-
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Cardiopulmonary bypass and complement activation

Anaesthesia, 1982
Summary Complement is activated during cardiopulmonary bypass with consumption of the complement components C3, C4 and factor B. This takes place when either a bubble or membrane oxygenator is used and is not affected by steroid therapy.
H M, Jones, N, Matthews, R S, Vaughan, J M, Stark   +3 more
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