Results 311 to 320 of about 248,487 (359)

Effect of Concanavalin A on the Classical Complement Pathway

The Journal of Immunology, 1977
Abstract Lysis of sheep erythrocytes (E) sensitized with anti-Forssman antiserum (EA) is inhibited by the action of concanavalin A (Con A) on whole guinea pig complement (GPC). The degree of inhibition observed for a given quantity of GPC was dependent on the Con A concentration.
J J, Langone, M D, Boyle, T, Borsos
openaire   +2 more sources

Will a hyperactive classical complement pathway exacerbate autoimmune diseases?

Autoimmunity Reviews, 2023
Peer ...
Okrój, Marcin, Rodríguez de Córdoba, Santiago   +1 more
openaire   +3 more sources

Control of the classical and the MBL pathway of complement activation

Molecular Immunology, 2000
The activation of complement via the mannan-binding lectin (MBL) pathway is initiated by the MBL complex consisting of the carbohydrate binding molecule, MBL, two associated serine proteases, MASP-1 and MASP-2, and a third protein, MAp19. In the present report we used an assay of complement activation specifically reflecting the physiological activity ...
Petersen, Steen Vang, Thiel, S, Jensen, L, Vorup-Jensen, T, Koch, C, Jensenius, J C   +5 more
openaire   +5 more sources

Interaction of fucoidan with the complement proteins of the classical pathway

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2003
Fucoidan inhibits complement by mechanisms that so far remain to be unraveled, and the objective of this work was to delineate the mode of inhibition by this sulfated polysaccharide. For that purpose, low molecular weight fractions of algal (Ascophyllum nodosum) fucoidan containing the disaccharide unit [-->3)-alpha-L-Fuc(2SO3(-))-(1-->4)-alpha-L-Fuc(2,
Tissot, B., Montdargent, B., Chevolot, L., Varenne, A., Descroix, S., Gareil, P., Daniel, Régis   +6 more
openaire   +4 more sources

A novel ELISA for the evaluation of the classical pathway of complement

Journal of Immunological Methods, 1998
Assessment of the overall function of the classical pathway of complement is traditionally performed by the hemolytic titration assay CH50. In the present study, we established a novel method for the quantitation of complement activity by measuring the deposition of C1q, C4, C3 and C9 on solid-phase IgM by an enzyme-linked immunosorbent assay (ELISA ...
Otto Götze, A Wittig, Jörg Zwirner, E Kremmer   +3 more
openaire   +3 more sources

Activation of the classical complement pathway by BioRex-70

Immunology Letters, 1987
The cation exchange resin BioRex-70 was able to activate the classical complement pathway in human serum at 37 degrees C over the resin concentration range 0-5% (v/v). Using zymosan-treated human serum, it was found that the activation proceeded as far as complement protein C3.
S.B. Easterbrook-Smith, R.J. Vandenberg
openaire   +3 more sources

The Classical Pathway of Complement [PDF]

, 2016
Complement evolved in parallel with coagulation as part of the primordial explosion of life in the Cambrian era. Remnants of this connection still exist in vertebrate animals as well as invertebrates. A classic example is the horseshoe crab, Limulus polyphemus, still sought after by scientists today to test substances for traces of bacterial ...
openaire   +1 more source

An immunofluorescence assay for complement activation by the classical pathway

Journal of Immunological Methods, 1981
The functional integrity of classical complement pathway components was determined by an immunofluorescence (IFL) assay based on the capacity of cytoskeletal intermediate filaments (IMF) to bind C1q and to activate the complement pathway. The assay uses IMF-rich capillary endothelium of human term placentae as complement-activating substrate.
S. Meri, M. Rhen, Ewert Linder
openaire   +3 more sources

complement classical and lectin pathways

2010
The classical and lectin pathways of complement are major recognition systems of innate immunity that are found in mammals and other animal species. By means of several multimolecular proteases – C1, the mannan-binding lectin (MBL)–MBL-associated serine protease 2 (MBL–MASP-2) and the ficolin–MASP-2 complexes – each comprising a recognition protein and
Thielens, Nicole, Gaboriaud, Christine, Thiel, Steffen   +2 more
openaire   +3 more sources

Identification of the activator system for antibody to Toxoplasma as the classical complement pathway.

Journal of Infectious Diseases, 1980
In view of the many recent advances in our understanding of the composition and function of the complement system, it was decided to apply this newer knowledge to an investigation of the heat-labile activator required for the action of antibody to ...
R. Schreiber, H. Feldman
semanticscholar   +1 more source