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Agroclavine hydroxylase of Claviceps purpurea

Biochimica et Biophysica Acta (BBA) - General Subjects, 1970
Abstract The 60–80% ammonium sulfate fraction of Claviceps purpurea PRL 1980 catalyzed the conversion of agroclavine to elymoclavine. The reaction required NADPH. EDTA and cyanide inhibited the reaction. Agroclavine increased the rate of oxidation of NADPH. The increase in rate was 4.8 μmoles NADPH per h mg protein at 25° and pH 7.0.
J C, Hsu, J A, Anderson
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Host infection by Claviceps purpurea

Transactions of the British Mycological Society, 1980
Infection of wheat and rye by Claviceps purpurea and its subsequent sphacelial development was investigated by light and electron microscopy. Penetration occurred via the stigma and ovary wall and colonization was intercellular. Pectolytic enzymes were detected both in submerged cultures of the fungus and in infected ovaries and may play a part in ...
Barbara I. Shaw, P.G. Mantle
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Delimitation of cryptic species inside Claviceps purpurea

Fungal Biology, 2015
Claviceps purpurea is an ovarian parasite infecting grasses (Poaceae) including cereals and forage plants. This fungus produces toxic alkaloids and consumption of contaminated grains can cause ergotism in humans and other mammals. Recent molecular genetics studies have indicated that it included three cryptic species (G1, G2, G3).
Sylvie Pažoutová   +4 more
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Parasitic differentiation of Claviceps purpurea

Transactions of the British Mycological Society, 1980
The origin and progress of differentiation of sclerotial tissues of Claviceps purpurea within the parasitic sphacelium is described. Light microscopy revealed early differentiation adjacent to conidiogenous tissue in a superficial region 12 days after inoculation.
Barbara I. Shaw, P.G. Mantle
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Enzymatic Methylation of Nicotinamide by Claviceps purpurea

Journal of Pharmaceutical Sciences, 1964
Soluble supernatant fractions from two strains of Claviceps purpurea were tested for the presence of enzymes capable of methylating tryptamine, 5-hydroxytryptamine, tyramine, and nicotinamide. The latter compound was the only one of the substrates tested in which methylation took place.
R C, AUDETTE   +2 more
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Succinate dehydrogenase in the ergot fungus claviceps purpurea☆

Biochimica et Biophysica Acta, 1963
Abstract 1. 1. A study of electron transport by cell extracts of the parasitic fungus Claviceps purpurea revealed a succinate dehydrogenase (EC 1.3.99.1) which appeared unique among mammalian and microbial succinate dehydrogenases so far studied in respect to ease of solubilization, solubility and stability, freedom from cytochrome contamination,
J K, MCDONALD   +3 more
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Semicontinuous cultivation of immobilized Claviceps purpurea

Applied Microbiology and Biotechnology, 1984
Mycelia of Claviceps purpurea CBS 164.59 were immobilized in 2%, 4%, and 8% calcium alginate. Alkaloid production by free cells declined after 60 days, while immobilized cells retained their activity for 200 days. The cumulative alkaloid production for all fermentation cycles using 8% calcium alginate immobilized mycelia was 25 times higher than that ...
Bettina Kopp, H. J. Rehm
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Extrachromosomal genetics of Claviceps purpurea

Current Genetics, 1983
In order to establish a system for gene cloning in the ergot fungus Claviceps purpurea mitochondrial DNA was purified from two wild strains and four descendants of production strains. A characterization of the mt DNA with respect to buoyant density, molecular weight (45 kb corresponding to acontour lenght of 14 μm) showed no differences.
P, Tudzynski, A, Düvell, K, Esser
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A GLUCAN PRODUCED BY CLAVICEPS PURPUREA

Canadian Journal of Chemistry, 1963
A glucan is produced by pure cultures of Claviceps purpurea (Fr.) Tul., PRL 1980, and its formation appears to be related to the accumulation of other shunt products such as ergot alkaloids, polyols, and trehalose. The glucan consists of β-D-glucopyranosyl units, most of which constitute a (1 → 3)-linked main chain.
A. S. Perlin, W. A. Taber
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Application of oxygen vectors to Claviceps purpurea cultivation

Applied Microbiology and Biotechnology, 2001
The application of a two-phase fermentation system for the production of ergot peptide alkaloids by Claviceps purpurea is described. Perfluorocarbons (PFC) are used as oxygen vectors in Claviceps fermentation for the first time. In shake-flask cultivations, the inclusion of PFC in the medium brings about a five-fold increase in the total alkaloid ...
M, Menge, J, Mukherjee, T, Scheper
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