Results 251 to 260 of about 750,149 (308)
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Cloning by function: expression cloning in mammalian cells
Trends in Pharmacological Sciences, 1994malian protein, the most important decision is the selection of a suitable strategy. Until recently, cloning re- quired prior purification of the pro- tein, and the corresponding cDNA was isolated by one of two approaches: (1) Micro-sequencing of all or parts of the purified protein was used to generate nucleic acid probes for ...
H, Simonsen, H F, Lodish
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Clones of alloreactive T cells
Nature, 1978Abstract Previous observations from our lab and many others have suggested that MLR determinants are composed of I region gene products. We extended our original observations by studying the reactivities of responder cells obtained after in vitro primary stimulation (primed responder cells = PRC) with selected stimulator cells.
Fathman, C G, Hengartner, H
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Diabetes, 1996
The role of T-cells in the pathogenesis of IDDM has been an area of much interest, and investigators have recently acquired new tools for studies on T-cells with the advent of T-cell clones that are reactive with islet antigens. Derived from NOD mice, diabetogenic T-cell lines and clones have for the most part been CD4+ and T-helper 1 (Th1)-like in ...
K, Haskins, D, Wegmann
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The role of T-cells in the pathogenesis of IDDM has been an area of much interest, and investigators have recently acquired new tools for studies on T-cells with the advent of T-cell clones that are reactive with islet antigens. Derived from NOD mice, diabetogenic T-cell lines and clones have for the most part been CD4+ and T-helper 1 (Th1)-like in ...
K, Haskins, D, Wegmann
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Embryonic and somatic cell cloning
Reproduction, Fertility and Development, 1999Revolutionary opportunities in biology, medicine and agriculture arise from the observation that offspring are obtained after nuclear transfer if somatic donor cells are induced to become quiescent. Exploitation of many of these opportunities will depend upon optimizing procedures for nuclear transfer.
I, Wilmut, L, Young, K H, Campbell
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From Clones of Cells to Cloned Genes and the Proteinpaedia
Scandinavian Journal of Immunology, 2005AbstractThis brief description follows the association of the author with Ivan Lefkovits from 1971 until this volume. Sketches of our mutual interests are included. Time periods in California, Basel and Texas are described. Decisions about preparing new tools for clonal analysis are elucidated, and experimental approaches leading to the x‐omic ...
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Modular cloning in plant cells
Trends in Plant Science, 2005New plant genes are being discovered at a rapid pace. Yet, in most cases, their precise function remains elusive. The recent advent of recombinational cloning techniques has significantly improved our ability to investigate gene functions systematically.
Karimi, Mansour +2 more
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Anticytomegalovirus T-Cell Clones
New England Journal of Medicine, 1996To the Editor: The article by Walter et al. (Oct. 19 issue)1 provides important evidence that the infusion of donor–derived CD8+ cytotoxic T-cell clones specific for human cytomegalovirus proteins can promptly reconstitute cellular immunity against human cytomegalovirus in recipients of allogeneic bone marrow, thus reducing the risk of morbidity and ...
Locatelli, F, Maccario, R, Gerna, G
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Single cell sorting and cloning
Journal of Immunological Methods, 2000Cell sorters now allow the selection of cells and other bodies according to a range of quite diverse criteria. The additional refinement that allows the sorting of individual cells based on these criteria has seen application in many fields of research.
F L, Battye, A, Light, D M, Tarlinton
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