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Cloning of fresh lymphoma cells
Medical and Pediatric Oncology, 1981AbstractWe attempted to induce in vivo clonal growth of neoplastic lymphoid cells from fresh specimens of involved tissue from 22 patients with non‐Hodgkin lymphoma (NHL). Conditioned media derived from two human B‐lymphocyte tissue culture lines were tested for their ability to promote colony growth.
M, Nishikori +3 more
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The Evolution of B-Cell Clones
1990This chapter identifies three forms of B-cell memory: (a) B blasts which characterize the established stage of the follicular response to TD antigens, (b) recirculating memory B cells, and (c) non-recirculating memory B cells of the marginal zones of the spleen and equivalent areas of other secondary lymphoid organs.
I C, MacLennan +4 more
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Cell Biology International Reports, 1978
Abstract The major problem in studying gene expression in eukaryotic organisms is the complexity of the eukaryotic genome. We would like to have the same detailed understanding of the genetics of eukaryotes as has been obtained for λ bacteriophage but are faced with the daunting statistic that the human genome could accommodate nearly 100,000 times ...
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Abstract The major problem in studying gene expression in eukaryotic organisms is the complexity of the eukaryotic genome. We would like to have the same detailed understanding of the genetics of eukaryotes as has been obtained for λ bacteriophage but are faced with the daunting statistic that the human genome could accommodate nearly 100,000 times ...
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2003
Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1, 2). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended periods (3).
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Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1, 2). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended periods (3).
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1987
Publisher Summary This chapter describes techniques for obtaining monoclonal lines of antigen-specific T cells. Murine antigen-reactive T cell clones can be divided into three categories, based on their functional phenotype (cytolytic vs noncytolytic), and their ability to proliferate in response to antigen in the absence of an exogenous source of ...
A, Livingstone, C G, Fathman
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Publisher Summary This chapter describes techniques for obtaining monoclonal lines of antigen-specific T cells. Murine antigen-reactive T cell clones can be divided into three categories, based on their functional phenotype (cytolytic vs noncytolytic), and their ability to proliferate in response to antigen in the absence of an exogenous source of ...
A, Livingstone, C G, Fathman
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Ageing of Clones of Mammalian Cells
Nature, 1973The chief purpose of this article is to review some recent work on the ageing of mammalian cells and to comment on the theoretical interpretation of the experimental findings. No claim will be made that cellular ageing is a primary cause of the ageing changes observed in whole animals; the evidence supporting such a causal connexion is inconclusive.
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1984
T cell clones are useful models for studying lymphocyte function both at the level of the individual cell and in interacting systems. Murine cytolytic and non- cytolyic T cell clones have been obtained with relative ease, and the particular procedure used to derive and maintain T cell clones may influence profoundly the characteristics of the resulting
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T cell clones are useful models for studying lymphocyte function both at the level of the individual cell and in interacting systems. Murine cytolytic and non- cytolyic T cell clones have been obtained with relative ease, and the particular procedure used to derive and maintain T cell clones may influence profoundly the characteristics of the resulting
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Blood, 2013
In this issue of Blood , Stevanovic et al demonstrate a possible link between cytomegalovirus (CMV) infection and the induction of CD4-dependent HLA-DP–directed colonic graft-versus-host disease (GVHD).[1][1] Their data suggest that virus-specific T cells mediate this effect via cytokine ...
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In this issue of Blood , Stevanovic et al demonstrate a possible link between cytomegalovirus (CMV) infection and the induction of CD4-dependent HLA-DP–directed colonic graft-versus-host disease (GVHD).[1][1] Their data suggest that virus-specific T cells mediate this effect via cytokine ...
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Handmade Somatic Cell Cloning in Cattle
2006Apart from the biological and ethical problems, technical difficulties also hamper the improvement and widespread application of somatic cell nuclear transfer (NT). Recently introduced zona-free procedures may offer a solution for the latter problem. The most radical approach of these techniques is the so-called handmade cloning (HMC).
Vajta, Gabor +2 more
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Somatic Cell Cloning without Micromanipulators
Cloning, 2001Until now, micromanipulators have been regarded as indispensable for somatic cell nuclear transfer. This paper describes an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Blastocyst
Vajta, G. +4 more
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