Results 91 to 100 of about 814,547 (292)

A Compensatory Mutation Provides Resistance to Disparate HIV Fusion Inhibitor Peptides and Enhances Membrane Fusion [PDF]

, 2013
Fusion inhibitors are a class of antiretroviral drugs used to prevent entry of HIV into host cells. Many of the fusion inhibitors being developed, including the drug enfuvirtide, are peptides designed to competitively inhibit the viral fusion protein ...
A Adachi, A Cole, A Cole, A Cole, Alan J. Waring, Alexander M. Cole, AM Barrio, AM Cole, Amy L. Cole, C Baldwin, C Baldwin, C Fuhrman, CR Eade, CT Wild, D Chan, D Eggink, E Micewicz, E Poveda, G Melikyan, GB Fields, J Kimpton, J Reeves, K Miyauchi, L Furci, Lisa C. Rohan, LJ Reed, M Caffrey, Matthew P. Wood, N Ray, Patrick M. Tarwater, Phalguni Gupta, Piotr Ruchala, Preston Marx, RP Lamers, S Gallo, S Owen, Shan-Lu Liu, T Chang, VD Trivedi, X Wei, Y He, Z Liu   +41 more
core   +3 more sources

Recombinant expression library of Pyrococcus furiosus constructed by high-throughput cloning: a useful tool for functional and structural genomics

Frontiers in Microbiology, 2015
Hyperthermophile Pyrococcus furiosus grows optimally near 100°C and is an important resource of many industrial and molecular biological enzymes. To study the structure and function of Pyrococcus furiosus proteins at whole genome level, we constructed ...
Hui eYuan, Li ePeng, Zhong eHan, Juan-Juan eXie, Xi-Peng eLiu   +4 more
doaj   +1 more source

YAP1::TFE3 mediates endothelial‐to‐mesenchymal plasticity in epithelioid hemangioendothelioma

Molecular Oncology, EarlyView.
The YAP1::TFE3 fusion protein drives endothelial‐to‐mesenchymal transition (EndMT) plasticity, resulting in the loss of endothelial characteristics and gain of mesenchymal‐like properties, including resistance to anoikis, increased migratory capacity, and loss of contact growth inhibition in endothelial cells.
Ant Murphy, Samuel Hartzler, Paula A. Vargas Carranza, Shyaman Jayasundara, Madison E. Yates, Nimod D. Janson, Bozhi Liu, Annaleigh Benton, Majid Kazemian, Jason A. Hanna   +9 more
wiley   +1 more source

A novel platform for the production of nonhydroxylated gelatins based on the methylotrophic yeast Hansenula polymorpha [PDF]

, 2007
The use of yeast as a host for heterologous expression of proteins that are normally derived from animal tissue is a promising way to ensure defined products that are devoid of potential harmful animal side products.
Boer, Arjo L. de,, Geerlings, Torsten H.,, Klei, Ida J. van der,, Lunenborg, Marcel G.J.,, Veenhuis, Marten,   +4 more
core   +2 more sources

Bioterrorismo e a facilidade de acesso à biotecnologia e seus insumos

Revista Bioética
Os fundamentalismos surgiram no Ocidente a partir de questões religiosas e posteriormente difundiram-se para outras partes do mundo tomando outras conotações, principalmente políticas. As técnicas de manipulação genética difundiram-se pelas universidades,
Cesar Koppe Grisolia
doaj  

High-throughput FastCloning technology: A low-cost method for parallel cloning

PLoS ONE, 2022
FastCloning, a reliable cloning technique for plasmid construction, is a widely used protocol in biomedical research laboratories. Only two-step molecular manipulations are required to add a gene (cDNA) of interest into the desired vector.
Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su   +9 more
doaj  

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Isolation and characterization of Pisum sativum apyrases, PsNTP9 and PsNTP9-DM, cloned and expressed in Escherichia coli [PDF]

, 2019
Adenosine triphosphate (ATP) is widely known as a fuel source for many biochemical processes, and to a lesser degree also as a signaling molecule in plants and animals.
Wallen, Michael Andrew, Jr
core   +1 more source

PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism

Molecular Oncology, EarlyView.
This study investigated how PYCR1 inhibition in bone marrow stromal cells (BMSCs) indirectly affects multiple myeloma (MM) cell metabolism and viability. Culturing MM cells in conditioned medium from PYCR1‐silenced BMSCs impaired oxidative phosphorylation and increased sensitivity to bortezomib.
Inge Oudaert, Lauren van den Broecke, Osman Aksoy, Judith Lind, Sonia Vallet, Arne Van der Vreken, Gamze Ates, Ann Massie, Ken Maes, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Klaus Podar, Eline Menu   +13 more
wiley   +1 more source

Cloning of a Carcinoembryonic Antigen Gene Family Member Expressed in Leukocytes of Chronic Myeloid Leukemia Patients and Bone Marrow [PDF]

, 1990
The carcinoembryonic antigen (CEA) gene family belongs to the immunoglobulin superfamily and can be subdivided into the CEA and pregnancy-specific glycoprotein subgroups.
Berling, Barbara, Brombacher, Frank, Buchegger, Franz, Grunert, Fritz, Kleist, Sabine von, Kolbinger, Frank, Thompson, John A., Zimmermann, Wolfgang   +7 more
core   +1 more source