Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning
Frontiers in Bioengineering and Biotechnology, 2020 Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan +5 more
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Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students [PDF]
Journal of Microbiology & Biology Education, 2023 Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes.
Mihaela Bejenari +5 more
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Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site [PDF]
BioTechniques, 2019 Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal +4 more
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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering [PDF]
BMC Biotechnology, 2010 Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm Morten HH
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Protocol: Precision engineering of plant gene loci by homologous recombination cloning in
Plant Methods, 2005 Plant genome sequence data now provide opportunities to conduct molecular genetic studies at the level of the whole gene locus and above. Such studies will be greatly facilitated by adopting and developing further the new generation of genetic ...
Göttgens Berthold +2 more
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‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes [PDF]
Current Issues in Molecular BiologyTo clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. Some cloning vectors have been developed for the cloning of PCR products, including blunt-end and T-A cloning. However, different plasmids are required
Xingli Zhang +4 more
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Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses
Microbiology Spectrum, 2023 Enteroviruses (EVs) have been associated with several human diseases. Due to their continuous emergence and divergence, EV species have generated more than 100 types and recombinant strains, increasing the public health threat caused by them.
Won-Suk Choi +14 more
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Evaluating the Performance of Clone Detection Tools in Detecting Cloned Co-change Candidates [PDF]
Journal of Systems and Software Volume 187, May 2022, 111229, 2022 Co-change candidates are the group of code fragments that require a change if any of these fragments experience a modification in a commit operation during software evolution. The cloned co-change candidates are a subset of the co-change candidates, and the members in this subset are clones of one another.
arxiv +1 more source
A practical comparison of ligation-independent cloning techniques. [PDF]
PLoS ONE, 2013 The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and ...
Julian Stevenson +3 more
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Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method [PDF]
PeerJ, 2018 Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min.
Lendl Tan +3 more
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