Results 11 to 20 of about 5,041,968 (259)

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

Frontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan, Yanjing Zeng, Wentao Shen, Decai Tuo, Xiaoying Li, Peng Zhou   +5 more
doaj   +2 more sources

Porcine SLITRK1: Molecular cloning and characterization [PDF]

FEBS Open Bio, 2014
The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The genomic organization of SLITRK1 lacks introns, as does its human and mouse counterparts.
Larsen, Knud Erik, Momeni, Jamal, Farajzadeh, Leila, Bendixen, Christian   +3 more
openaire   +6 more sources

Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli. [PDF]

Plant Methods, 2005
© 2005 Roden et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and ...
Göttgens, Berthold, Mutasa-Gottgens, Euphemia, Roden, Laura C   +2 more
core   +7 more sources

Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses

Microbiology Spectrum, 2023
Enteroviruses (EVs) have been associated with several human diseases. Due to their continuous emergence and divergence, EV species have generated more than 100 types and recombinant strains, increasing the public health threat caused by them.
Won-Suk Choi, Sol Oh, Khristine Joy C. Antigua, Ju Hwan Jeong, Beom Kyu Kim, Yu Soo Yun, Da Hyeon Kang, Seong Cheol Min, Byung-Kwan Lim, Won Seop Kim, Ji-Hyuk Lee, Eung-Gook Kim, Young Ki Choi, Yun Hee Baek, Min-Suk Song   +14 more
doaj   +1 more source

'Two in One' Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion-Ligation and Screening of Positive Recombinants by Unaided Eyes. [PDF]

Curr Issues Mol Biol
To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. Some cloning vectors have been developed for the cloning of PCR products, including blunt-end and T-A cloning. However, different plasmids are required
Zhang X, Teng C, Lyu K, Lyu S, Fan Y.
europepmc   +2 more sources

A practical comparison of ligation-independent cloning techniques. [PDF]

PLoS ONE, 2013
The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and ...
Julian Stevenson, James R Krycer, Lisa Phan, Andrew J Brown   +3 more
doaj   +1 more source

Evaluating the Performance of Clone Detection Tools in Detecting Cloned Co-change Candidates [PDF]

Journal of Systems and Software Volume 187, May 2022, 111229, 2022
Co-change candidates are the group of code fragments that require a change if any of these fragments experience a modification in a commit operation during software evolution. The cloned co-change candidates are a subset of the co-change candidates, and the members in this subset are clones of one another.
arxiv   +1 more source

Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method [PDF]

PeerJ, 2018
Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min.
Lendl Tan, Emily J. Strong, Kyra Woods, Nicholas P. West   +3 more
doaj   +2 more sources

Molecular cloning of a bovine cathepsin [PDF]

Biochemical Journal, 1985
A cDNA clone for a thiol endoproteinase has been isolated from a bovine heart cDNA library by using a mixture of 32 synthetic oligonucleotides as a hybridization probe. The inserted region is 672 base pairs in length. It contains a sequence encoding the C-terminal region of a protein that is homologous to rat liver cathepsins B and H and to plant thiol
N J, Gay, J E, Walker
openaire   +3 more sources

Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students

Journal of Microbiology & Biology Education, 2023
Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes.
Mihaela Bejenari, Line Nielsen, Eva Mie Lang Spedtsberg, Mikkel Rank Nielsen, Tobias Bruun Pedersen, Jens Laurids Sørensen   +5 more
doaj   +1 more source