Results 11 to 20 of about 822,631 (292)

HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs. [PDF]

PLoS ONE, 2014
Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in ...
Ming V Li, Dip Shukla, Brian H Rhodes, Anjali Lall, Jingmin Shu, Branden S Moriarity, David A Largaespada   +6 more
doaj   +1 more source

QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids [PDF]

, 2015
Background Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning.
Jajesniak, P., Wong, T.S.
core   +2 more sources

PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs

BioTechniques, 2023
With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran, Ryan D Hallam, Marvel Megaly, Anel Turgambayeva, Aleksandar Necakov   +4 more
doaj   +1 more source

Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site [PDF]

, 2018
Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Beyaert, Rudi, De Schamphelaire, Wouter, Staal, Jens   +2 more
core   +1 more source

The Cloning of the Human Tumor Supressor Gene INGI: DNA Cloning into Plasmid Vector and DNA Analysis by Restriction Enzymes

Journal of Dentistry Indonesia, 2015
DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari, Mamoru Ouchida, Mehmet Gunduz   +2 more
doaj   +1 more source

Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting

iScience, 2019
Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé, Arnaud Taton, James W. Golden   +2 more
doaj   +1 more source

Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. [PDF]

PLoS ONE, 2013
The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites.
Stephan Kirchmaier, Katharina Lust, Joachim Wittbrodt   +2 more
doaj   +1 more source

HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review

Biomedicines, 2021
Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates ...
Kiichi Hirota
doaj   +1 more source

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants [PDF]

, 1989
Transposition of the Anthirrinum majus Tam3 element and the Zea mays Ac element has been monitored in petunia and tobacco plants. Plant vectors were constructed with the transposable elements cloned into the leader sequence of a marker gene ...
A Day, A Herrmann, A Hoekema, A Hudson, AH Shirsat, B Baker, B Baker, C Martin, C Martin, C O'Reilly, Caius M. T. Rommens, D Inzé, DC Baulcombe, F Sanger, G Ditta, H Sommer, H. John J. Nijkamp, J Gielen, J Paz-Ares, Jacques Hille, JDG Jones, JI Yoder, Jie Gao, JM Goddard, KC Cone, LO Vodkin, M Motto, M-A Grandbastien, MA Sluys Van, Michel A. Haring, NV Fedoroff, P Maliga, P Nevers, P Vlaming De, R Carpenter, RA Jefferson, S Knapp, SL Dellaporta, T Maniatis, Tjeerd Volbeda, U Behrens, WD Sutton   +41 more
core   +13 more sources

Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site

BioTechniques, 2019
Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal, Kübra Alci, Wouter De Schamphelaire, Martine Vanhoucke, Rudi Beyaert   +4 more
doaj   +1 more source