biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the <i>ACTB</i> locus. [PDF]
Pelosse M, Marcia M.
europepmc +1 more source
Transcript Identification Using Arrayed Hydrogels With TrapFISH
TrapFISH is a novel method for high‐throughput single‐cell transcriptomics. It embeds cells in hyaluronic acid hydrogel beads and uses probe hybridization to quantify custom gene panels. By arraying hydrogels in microfluidic devices, cell barcoding is removed, enhancing scalability for studying cellular heterogeneity and rare cell states by targeting ...
David B. Morse+5 more
wiley +1 more source
DAPE cloning with modified primers for producing designated lengths of 3' single-stranded ends in PCR products. [PDF]
Lee S+5 more
europepmc +1 more source
Designing a multi-epitope universal vaccine for concurrent infections of SARS-CoV-2 and influenza viruses using an immunoinformatics approach. [PDF]
Mohammadipour S+4 more
europepmc +1 more source
A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9. [PDF]
García-Calvo L+4 more
europepmc +1 more source
Development of Novel ssDNA Aptamers for Detection of Receptor-Binding Domain of SARS-COV‑2. [PDF]
Auti A+8 more
europepmc +1 more source
Molecular Mechanisms of Somatic Cell Cloning and Other Assisted Reproductive Technologies in Mammals: Which Determinants Have Been Unraveled Thus Far?-Current Status, Further Progress and Future Challenges. [PDF]
Samiec M.
europepmc +1 more source
Development of the coronavirus reverse genetic system: Core technology for pathogenesis mechanisms research and vaccine/drug development. [PDF]
Zhang Y, Hou R, Wei Z, Yuan J, Zu S.
europepmc +1 more source