Results 251 to 260 of about 3,939,762 (298)
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Molecular Cloning of PCR Products
Current Protocols in Molecular Biology, 2001AbstractIt is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high‐quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified
M, Finney, P E, Nisson, A, Rashtchian
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Molecular Cloning of the Thyrotropin Receptor
Science, 1989The pituitary hormone thyrotropin, or thyroid-stimulating hormone (TSH), is the main physiological agent that regulates the thyroid gland. The thyrotropin receptor (TSHR) was cloned by selective amplification with the polymerase chain reaction of DNA segments presenting sequence similarity with genes for G protein-coupled receptors. Out of
Parmentier, Marc +8 more
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Molecular cloning of feline CD34
Veterinary Immunology and Immunopathology, 2003In humans, baboons, dogs and mice CD34 is a cell surface molecule that is expressed on primitive hematopoietic cells and in all these species CD34 positive cells can be used to effect long-term haematopoietic reconstitution. CD34 positive haematopoietic cells therefore provide a convenient and relatively small cell population to target when attempting ...
Yogalingam, G., Anson, D.
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Molecular Cloning of Steroid Hydroxylases
Endocrine Research, 1984Recombinant plasmids specific for bovine adrenocortical cytochromes P-450scc and P-45011 beta have been identified and characterized. Using these cDNA inserts as probes, it is found that tissue specificity of gene expression for these two proteins is as expected. Cytochrome P-450scc mRNA is found in adrenocortical and corpus luteum RNA while cytochrome
M E, John +3 more
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2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
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This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
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Human Genetics, 1977
Biochemical, biophysical and genetic studies of DNA segments of complex genomes are greatly facilitated by a variety of techniques, called molecular cloning of DNA, which permit propagation of single DNA segments of virtually any origin in bacterial cells.
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Biochemical, biophysical and genetic studies of DNA segments of complex genomes are greatly facilitated by a variety of techniques, called molecular cloning of DNA, which permit propagation of single DNA segments of virtually any origin in bacterial cells.
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Sarcolectin: Complete purification for molecular cloning
Biochimie, 1999A great variety of vertebrate cells contain detectable amounts of lectins, able to stimulate the initiation of cellular DNA synthesis. One of them, sarcolectin (SCL) can block interferon (IFN) action, by inhibiting the synthesis and the expression of the IFN dependent secondary proteins.
P H, Jiang, F, Chany-Fournier, C, Chany
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Molecular cloning of mouse thioredoxin reductases
Gene, 2000We screened clones for thioredoxin reductase genes with a degenerate PCR-based strategy and have isolated two novel cDNA clones from a mouse thymocyte cDNA library. These encode two distinct thioredoxin reductases (TrxR1 and TrxR2) with 499 and 527 amino acid (aa) residues and calculated molecular masses of 54.5 kDa and 56.8 kDa respectively.
H, Kawai, T, Ota, F, Suzuki, M, Tatsuka
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Molecular cloning and characterization of human WNT7B
International Journal of Oncology, 2001WNT signaling molecules are implicated in carcinogenesis and embryogenesis. Only partial coding sequence of human WNT7B is reported so far, and human genome draft sequence corresponding to the WNT7B gene in human chromosome 22q13 region is not available at present.
H, Kirikoshi, H, Sekihara, M, Katoh
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