Results 271 to 280 of about 814,547 (292)
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Human Genetics, 1977
Biochemical, biophysical and genetic studies of DNA segments of complex genomes are greatly facilitated by a variety of techniques, called molecular cloning of DNA, which permit propagation of single DNA segments of virtually any origin in bacterial cells.
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Biochemical, biophysical and genetic studies of DNA segments of complex genomes are greatly facilitated by a variety of techniques, called molecular cloning of DNA, which permit propagation of single DNA segments of virtually any origin in bacterial cells.
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Sarcolectin: Complete purification for molecular cloning
Biochimie, 1999A great variety of vertebrate cells contain detectable amounts of lectins, able to stimulate the initiation of cellular DNA synthesis. One of them, sarcolectin (SCL) can block interferon (IFN) action, by inhibiting the synthesis and the expression of the IFN dependent secondary proteins.
P H, Jiang, F, Chany-Fournier, C, Chany
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Molecular cloning of mouse thioredoxin reductases
Gene, 2000We screened clones for thioredoxin reductase genes with a degenerate PCR-based strategy and have isolated two novel cDNA clones from a mouse thymocyte cDNA library. These encode two distinct thioredoxin reductases (TrxR1 and TrxR2) with 499 and 527 amino acid (aa) residues and calculated molecular masses of 54.5 kDa and 56.8 kDa respectively.
H, Kawai, T, Ota, F, Suzuki, M, Tatsuka
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Molecular cloning of Drosophila TFIID subunits
Nature, 1994Transcription initiation factor TFIID is a multisubunit complex containing a TATA-box-binding factor (TFIID tau/TBP) and associated polypeptide factors (TAFs) with sizes ranging from M(r) approximately 20,000 to > 200,000. As a result of direct promoter interactions, TFIID nucleates the assembly of RNA polymerase II and other initiation factors into a ...
T, Kokubo +5 more
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Molecular Cloning and Characterization of Xenopus RGS5
Biochemical and Biophysical Research Communications, 2000We identified six genes that encode putative RGS proteins (XRGSI-VI) in developing Xenopus embryos using PCR amplification with degenerate primers corresponding to the conserved region (RGS domain) of known RGS proteins. RT-PCR analysis revealed that mRNAs of these XRGSs are differentially expressed during embryogenesis.
O, Saitoh +4 more
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Molecular cloning and characterization of human WNT8A
International Journal of Oncology, 2001WNT - beta-catenin - TCF pathway is involved in carcinogenesis and fetal development. Xenopus wnt-8 is one of the most potent Wnts with the capacity to activate beta-catenin - TCF pathway in the Xenopus axis duplication assay. Here, we have cloned and characterized WNT8A, a novel human homologue of Xenopus wnt-8.
T, Saitoh, M, Katoh
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Molecular Cloning of Lungfish Proopiomelanocortin cDNA
General and Comparative Endocrinology, 1999To investigate the evolution of proopiomelanocortin (POMC) from fish to tetrapods, nucleotide sequence of POMC cDNA from a lobe-finned fish, the African lungfish, was determined. POMC cDNA was prepared from lungfish pituitary glands. The POMC cDNA is composed of 1114 bp, excluding a poly-A tail, and encodes 255 amino acids (aa) including a signal ...
Y, Amemiya +3 more
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Molecular Cloning of Protein-Based Polymers
Biomacromolecules, 2006Protein-based biopolymers have become a promising class of materials for both biomedical and pharmaceutical applications, as they have well-defined molecular weights, monomer compositions, as well as tunable chemical, biological, and mechanical properties.
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