Results 271 to 280 of about 759,664 (294)

Molecular Cloning of Human Amidophosphoribosyltransferase

Biochemical and Biophysical Research Communications, 1993
The cDNA of human amidophosphoribosyltransferase (EC 2.4.2.14, ATase), which is the supposed regulatory allosteric enzyme of de novo purine nucleotide biosynthesis, has been cloned from human hepatoma (HepG2) cDNA library. The predicted open reading frame encodes a protein of 517 amino acids with a deduced molecular weight (Mr) of 57,398, which is ...
H, Iwahana, J, Oka, N, Mizusawa, E, Kudo, S, Ii, K, Yoshimoto, E W, Holmes, M, Itakura   +7 more
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Molecular Cloning of the Thyrotropin Receptor

Science, 1989
The pituitary hormone thyrotropin, or thyroid-stimulating hormone (TSH), is the main physiological agent that regulates the thyroid gland. The thyrotropin receptor (TSHR) was cloned by selective amplification with the polymerase chain reaction of DNA segments presenting sequence similarity with genes for G protein-coupled receptors. Out of
Parmentier, Marc, Libert, Frédérick, Maenhaut, Carine, Lefort, Anne, Gérard, Claude, Perret, Jason, Van Sande, Jacqueline, Dumont, Jacques, Vassart, Gilbert   +8 more
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Molecular Cloning of HumanFrizzled-6,

Biochemical and Biophysical Research Communications, 1998
The Frizzled genes encode receptors for WNTs, secreted glycoproteins implicated in development as well as in carcinogenesis. In this paper, we report molecular cloning of Hfz6, the human homologue of Mfz6. Nucleotide sequence analysis showed that the Hfz6 gene encodes the 706 amino-acid protein with seven transmembrane domains, a cystein-rich domain in
Masaru Katoh, Yutaka Atomi, Masaaki Terada, Momoki Hirai, Makoto Tokuhara   +4 more
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Molecular Cloning

2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
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Isolation and molecular cloning of a fish myeloperoxidase

Molecular Immunology, 2008
Myeloperoxidase (MPO) is a conspicuous enzyme in neutrophils of many fish species. Although the MPO gene has been identified in some fish species, the structure and functions of the protein remain to be determined in these vertebrates. In the present study, we isolated turbot neutrophil MPO from kidney cells by affinity chromatography, with Ulva rigida
Castro, Rosario, Piazzon de Haro, María Carla, Noia, Manuel, Leiro, José M., Lamas, Jesús   +4 more
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Molecular cloning and expression of internalin in Listeria

1994
Publisher Summary This chapter discusses the molecular cloning and expression of internalin in Listeria . Listeria monocytogenes is a ubiquitous gram-positive bacterium that causes severe infections in humans and in a variety of warm-blooded animals. This pathogen has emerged as an important agent of foodborne disease in the last decade.
Gaillard, Jean-Louis, Dramsi, Shaynoor, Berche, Patrick, Cossart, Pascale   +3 more
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Molecular cloning of mouse allantoicase cDNA

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2001
The uric acid degradation pathway is progressively lost during vertebrate evolution. In mammals, the end product of this catabolic pathway is allantoin and, therefore, no allantoicase should be present in mouse tissues. Surprisingly, we have found an expressed sequence tag (EST) from mouse testis with high similarity to allantoicase.
VIGETTI, DAVIDE, MONETTI C, BERNARDINI, GIOVANNI BATTISTA   +2 more
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Molecular cloning of lupin leghemoglobin cDNA

Molecular Biology Reports, 1987
Poly(A)+ RNA isolated from root nodules of yellow lupin (Lupinus luteus, var. Ventus) has been used as a template for the construction of a cDNA library. The ds cDNA was synthesized and inserted into the Hind III site of plasmid pBR 322 using synthetic Hind III linkers.
Konieczny, A, Jensen, E O, Marcker, K A, Legocki, A   +3 more
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Molecular Cloning of Steroid 21-Hydroxylase

Endocrine Research, 1984
Congenital adrenal hyperplasia due to 21-hydroxylase (21-OH) deficiency is HLA-linked. The haplotype HLA-(A3);Bw47;DR7 is strongly associated with 21-OH deficiency and always carries a null allele at the complement C4A (Rodgers) locus. It seemed likely that this haplotype carries a deletion encompassing both the C4A and 21-OH loci. We hypothesized that
Bo Dupont, Bo Dupont, Maria I. New, Maria I. New, Perrin C. White, Perrin C. White   +5 more
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Sarcolectin: Complete purification for molecular cloning

Biochimie, 1999
A great variety of vertebrate cells contain detectable amounts of lectins, able to stimulate the initiation of cellular DNA synthesis. One of them, sarcolectin (SCL) can block interferon (IFN) action, by inhibiting the synthesis and the expression of the IFN dependent secondary proteins.
Françoise Chany-Fournier, Charles Chany, Pan Hong Jiang   +2 more
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