Results 21 to 30 of about 2,398 (50)
Drug Design, Development and Therapy, 2020 Hassan Dana, 1, 2,* Ghanbar Mahmoodi Chalbatani, 1,* Elahe Gharagouzloo, 1 Seyed Rouhollah Miri, 1 Fereidoon Memari, 1 Reza Rasoolzadeh, 3 Mohammad Reza Zinatizadeh, 3 Peyman Kheirandish Zarandi, 3 Vahid Marmari 2 1Cancer Research Center, Cancer ...
Dana H +8 more
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Streamlined assembly of cloning and genome editing vectors for genus Clostridium
iScience, 2023 Summary: Reported herein is a new set of vectors designed to streamline molecular cloning and genome editing by exploiting modern cloning methods. The new vectors build on the existing pMTL8000 vectors that have been a staple of Clostridium research for ...
Tom S. Bailey +3 more
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Recombination-assisted megaprimer (RAM) cloning
MethodsX, 2014 No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide ...
Jacques Mathieu +2 more
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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR. [PDF]
PLoS ONE, 2017 The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental ...
Faqing Huang +2 more
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BMC Research Notes, 2012 Background Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an incorrect ...
Orlova Nadezhda A +2 more
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Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique
JOIV: International Journal on Informatics Visualization, 2021 Bioinformatics has developed into an application tool for basic and applied research in the biomedical and biotechnology field. Polymerase Chain Reaction (PCR) is a common technique in the molecular area that has always involved bioinformatics science ...
- Mardalisa +5 more
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BMC Biotechnology, 2011 Background Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. Results Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any ...
Lu Jia +5 more
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TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method
Frontiers in Bioengineering and Biotechnology, 2023 Molecular cloning is used in a wide variety of biological and medical research. Here, we developed a rapid and efficient DNA-assembling method for routine laboratory work.
Fang Yu +8 more
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Porcine SLITRK1: Molecular cloning and characterization
FEBS Open Bio, 2014 The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The
Knud Larsen +3 more
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iScience, 2019 Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé +2 more
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