Results 21 to 30 of about 814,547 (292)
Molecular cloning and characterization of B-cadherin, a novel chick cadherin. [PDF]
, 1991 Calcium-dependent cell-cell adhesion is mediated in large part by a set of homologous integral membrane glycoproteins termed cadherins. In this report, antibodies to conserved domains in previously described cadherins have been used to isolate cDNAs ...
Napolitano, EW +3 more
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BioTechniques, 2019 Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal +4 more
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BioTechniques, 2020 During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited.
Tomo Kondo, Shigehiko Yumura
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Optimal cloning of PCR fragments by homologous recombination in Escherichia coli. [PDF]
PLoS ONE, 2015 PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown
Ana Paula Jacobus, Jeferson Gross
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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
BMC Biotechnology, 2010 Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm Morten HH
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Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice
Czech Journal of Genetics and Plant Breeding, 2022 Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism,
Yu Li +6 more
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Metabolic Engineering Communications, 2016 Molecular Cloning Designer Simulator (MCDS) is a powerful new all-in-one cloning and genetic engineering design, simulation and management software platform developed for complex synthetic biology and metabolic engineering projects.
Zhenyu Shi, Claudia E. Vickers
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Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls [PDF]
, 2007 Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Klinghammer, Michaela, Tenhaken, Raimund
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Stem Cell Research & Therapy, 2019 Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu +13 more
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Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]
, 2016 Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng +7 more
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