Results 21 to 30 of about 822,631 (292)

An improved molecular tool for screening bacterial colonies using GFP expression enhanced by a Dictyostelium sequence

BioTechniques, 2020
During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited.
Tomo Kondo, Shigehiko Yumura
doaj   +1 more source

Optimal cloning of PCR fragments by homologous recombination in Escherichia coli. [PDF]

PLoS ONE, 2015
PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown
Ana Paula Jacobus, Jeferson Gross
doaj   +1 more source

Molecular cloning and characterization of B-cadherin, a novel chick cadherin. [PDF]

, 1991
Calcium-dependent cell-cell adhesion is mediated in large part by a set of homologous integral membrane glycoproteins termed cadherins. In this report, antibodies to conserved domains in previously described cadherins have been used to isolate cDNAs ...
Napolitano, EW, Reichardt, LF, Venstrom, K, Wheeler, EF   +3 more
core   +2 more sources

A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

BMC Biotechnology, 2010
Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm Morten HH
doaj   +1 more source

Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice

Czech Journal of Genetics and Plant Breeding, 2022
Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism,
Yu Li, Xiaomi Chen, Tao Lan, Jing Zhang, Ziheng Chen, Wenting Yang, Xinmei Lin   +6 more
doaj   +1 more source

Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization [PDF]

, 2013
Calreticulin (CRT) is a highly conserved and ubiquitously expressed Ca2+-binding protein in multicellular eukaryotes. As an endoplasmic reticulum-resident protein, CRT plays a key role in many cellular processes including Ca2+ storage and release ...
A Christensen, AJ Crofts, AM Hassan, Anna Suwińska, BA Kwiatkowski, BQ Huang, CM Williams, CN Nardi, CO Lim, DE Nelson, E Gasteiger, E Zuckerkandl, F Chen, H Jin, HJ Li, HJ Li, HQ Tian, J Denecke, J Felsenstein, J Li, JC Huang, JL Went Van, JR Nevins, Justyna Prusińska, K Hsieh, K Tamura, Krzysztof Gumowski, L Navazio, L Thelin, LI Gold, LL Ge, M Iwano, M Lenartowska, M Lenartowska, M Lenartowska, M Michalak, MA Larkin, Marta Lenartowska, MTM Willemse, N Blom, N Borisjuk, N Saitou, P Mariani, P Shaw, Robert Lenartowski, S Persson, S Persson, SF Altschul, SJ Coughlan, T Dresselhaus, T Ishimizu, UK Tirlapur, WA Wang, XL Ye, XY Jia, XY Jia, Y Fu, Y Saijo, YQ An, Z Li   +59 more
core   +2 more sources

Molecular Cloning Designer Simulator (MCDS): All-in-one molecular cloning and genetic engineering design, simulation and management software for complex synthetic biology and metabolic engineering projects

Metabolic Engineering Communications, 2016
Molecular Cloning Designer Simulator (MCDS) is a powerful new all-in-one cloning and genetic engineering design, simulation and management software platform developed for complex synthetic biology and metabolic engineering projects.
Zhenyu Shi, Claudia E. Vickers
doaj   +1 more source

Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system

Stem Cell Research & Therapy, 2019
Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu, Leqian Yu, Jianxiong Guo, Jinzhu Xiang, Zheng Zheng, Dengfeng Gao, Bingbo Shi, Haiyang Hao, Deling Jiao, Liang Zhong, Yu Wang, Jun Wu, Hongjiang Wei, Jianyong Han   +13 more
doaj   +1 more source

Local cloning of two product states [PDF]

, 2005
Local quantum operations and classical communication (LOCC) put considerable constraints on many quantum information processing tasks such as cloning and discrimination.
Mingsheng Ying, W. K. Wootters, Yuan Feng, Zhengfeng Ji   +3 more
core   +2 more sources

Molecular cloning of cat interleukin-12 [PDF]

Immunogenetics, 1997
The recently discovered interleukin-12 (IL-12) is a heterodimeric 70000 Mr cytokine composed of a disulphide-bonded 35000 Mr (p35) subunit and a 40000 Mr (p40) subunit. Produced mainly by activated monocytes/macrophages and B cells, it enhances NK/LAK cell cytolytic activity and stimulates cytotoxic T-cell responses and IFN-γ production.
Horzinek, M.C., Schijns, Virgil E.C.J., Wierda, C.M., Vahlenkamp, T.W.   +3 more
openaire   +3 more sources