Results 21 to 30 of about 5,041,968 (259)
HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs. [PDF]
PLoS ONE, 2014 Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in ...
Ming V Li +6 more
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Biomédica: revista del Instituto Nacional de Salud, 2008 Introducción. Los dos subgéneros en los cuales se divide el género Leishmania: Viannia y Leishmania, presentan diferencias significativas en las manifestaciones clínicas que causan, en su comportamiento de crecimiento en cultivos in vitro, en sus ...
Marcel Marín +4 more
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bioRxiv, 2017 Basic helix-loop-helix (bHLH) transcription factors play key roles in plant male reproduction. More than 14 bHLH proteins related to pollen development have been cloned from rice and Arabidopsis. However, little is known about the role of the bHLH family
Yongming Liu +8 more
semanticscholar +1 more source
BioTechniques, 2023 With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran +4 more
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QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids [PDF]
, 2015 Background Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning.
Jajesniak, P., Wong, T.S.
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Diversity of actinobacteria in the marshes of Ezzemoul and Djendli in northeastern Algeria [PDF]
, 2019 The main purpose of this research is to study the microbial diversity of actinobacteria, living in “Ezzemoul” and “Djendli” sebkhas soils. These salt lakes are situated in the east of Algeria and they are microbiologically underexploited.
Kitouni, Mahmoud, Smati, Maria
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Journal of Dentistry Indonesia, 2015 DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari +2 more
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Molecular cloning and characterization of human endothelial nitric oxide synthase [PDF]
, 1992 The constitutive calcium/calmodulin-dependent nitric oxide (NO) synthase expressed in vascular endothelium shares common biochemical and pharmacologic properties with neuronal NO synthase.
Ignarro +21 more
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Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site [PDF]
, 2018 Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Beyaert, Rudi +2 more
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Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. [PDF]
PLoS ONE, 2013 The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites.
Stephan Kirchmaier +2 more
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