Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls [PDF]
, 2007 Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Klinghammer, Michaela, Tenhaken, Raimund
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Arguments for Human Therapeutic Cloning
Biomolecules & Biomedicine, 2008 For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
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Sequence-specific protein aggregation generates defined protein knockdowns in plants [PDF]
, 2016 Protein aggregation is determined by short (5-15 amino acids) aggregation-prone regions (APRs) of the polypeptide sequence that self-associate in a specific manner to form beta-structured inclusions.
Aesaert, Stijn +16 more
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Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research. [PDF]
PLoS ONE, 2017 Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding ...
Mostafa Aboulela +7 more
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Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]
, 2016 Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng +7 more
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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR. [PDF]
PLoS ONE, 2017 The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental ...
Faqing Huang +2 more
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An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Recombination-assisted megaprimer (RAM) cloning
MethodsX, 2014 No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide ...
Jacques Mathieu +2 more
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The TelN/tos-assisted precise targeting of chromosome segments (TAPE)
Journal of Advanced Research, 2022 Introduction: Performing genomic large segmentation experiments will promote the annotation of complex genomic functions and contribute to the synthesis of designed genomes.
You-Zhi Cui +3 more
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Ion channels: structural basis for function and disease. [PDF]
, 1996 Ion channels are ubiquitous proteins that mediate nervous and muscular function, rapid transmembrane signaling events, and ionic and fluid balance. The cloning of genes encoding ion channels has led to major strides in understanding the mechanistic basis
Goldstein, SA
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