Results 31 to 40 of about 759,664 (294)

Molecular cloning and expression of human caldecrin [PDF]

FEBS Letters, 1996
Earlier we reported the primary structure of serum calcium‐decreasing factor (caldecrin) from rat pancreas, a protein which is considered to be a member of the elastase family. In this report, we describe the isolation of the two homologous cDNA clones encoding caldecrin from human pancreas, the structures of which are identical except for one base and
Mineko Tomomura, Izumi Yoshino, Takenori Noikura, Hirotaka Itoh, Takeyori Saheki, Akito Tomomura, Masashi Akiyama, Yasuho Nishii   +7 more
openaire   +3 more sources

Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells

STAR Protocols, 2022
Summary: Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures.
Ana Luiza Drumond-Bock, Magdalena Cybula, Luyao Wang, Lin Wang, Magdalena Bieniasz   +4 more
doaj  

Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization [PDF]

, 2013
Calreticulin (CRT) is a highly conserved and ubiquitously expressed Ca2+-binding protein in multicellular eukaryotes. As an endoplasmic reticulum-resident protein, CRT plays a key role in many cellular processes including Ca2+ storage and release ...
A Christensen, AJ Crofts, AM Hassan, Anna Suwińska, BA Kwiatkowski, BQ Huang, CM Williams, CN Nardi, CO Lim, DE Nelson, E Gasteiger, E Zuckerkandl, F Chen, H Jin, HJ Li, HJ Li, HQ Tian, J Denecke, J Felsenstein, J Li, JC Huang, JL Went Van, JR Nevins, Justyna Prusińska, K Hsieh, K Tamura, Krzysztof Gumowski, L Navazio, L Thelin, LI Gold, LL Ge, M Iwano, M Lenartowska, M Lenartowska, M Lenartowska, M Michalak, MA Larkin, Marta Lenartowska, MTM Willemse, N Blom, N Borisjuk, N Saitou, P Mariani, P Shaw, Robert Lenartowski, S Persson, S Persson, SF Altschul, SJ Coughlan, T Dresselhaus, T Ishimizu, UK Tirlapur, WA Wang, XL Ye, XY Jia, XY Jia, Y Fu, Y Saijo, YQ An, Z Li   +59 more
core   +2 more sources

Arguments for Human Therapeutic Cloning

Biomolecules & Biomedicine, 2008
For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
doaj   +1 more source

Accelerated Evolution of the ASPM Gene Controlling Brain Size Begins Prior to Human Brain Expansion [PDF]

, 2004
Primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by global reduction in cerebral cortical volume. The microcephalic brain has a volume comparable to that of early hominids, raising the possibility that some MCPH genes may have ...
Abeysinghe, Aota, Benson, Bernardi, Bond, Bond, Chen, Chenn, Chuzhanova, Clark, Crandall, Dewyse, do Carmo Avides, Duret, Endo, Evans, Galtier, Gould, Hughes, Jurka, Kent, Kouprina, Kreitman, McCreary, McDonald, Mochida, Morgenstern, Noskov, Polushin, Rehen, Riparbelli, Ripoll, Roberts, Rudiger, Sharp, Sharp, Tobias, Wood, Yang, Yang, Zhang   +40 more
core   +3 more sources

Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]

, 2016
Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng, Huang, Bei, Huang, Wen-Shu, Jia, Qin-qin, Nie, Pin, Wang, Ke-Jian, Wang, Tiehui, Zhang, Cai-Liang   +7 more
core   +1 more source

Molecular cloning of the genome of human spumaretrovirus [PDF]

Gene, 1987
DNA of human spumaretrovirus (HSRV) was cloned from both cDNA and from viral DNA into phage lambda and bacterial plasmid vectors. The recombinant plasmids harboring viral DNA were characterized by Southern blot hybridization and restriction mapping. Physical maps were constructed from cDNA and found to be colinear with the restriction maps obtained ...
Rethwilm, Axel, Darai, G., Rösen, A., Maurer, Bernd, Flügel, Rolf M.   +4 more
openaire   +4 more sources

Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research. [PDF]

PLoS ONE, 2017
Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding ...
Mostafa Aboulela, Yuji Tanaka, Kohji Nishimura, Shoji Mano, Mikio Nishimura, Sumie Ishiguro, Tetsuya Kimura, Tsuyoshi Nakagawa   +7 more
doaj   +1 more source

In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment

Drug Design, Development and Therapy, 2020
Hassan Dana, 1, 2,* Ghanbar Mahmoodi Chalbatani, 1,* Elahe Gharagouzloo, 1 Seyed Rouhollah Miri, 1 Fereidoon Memari, 1 Reza Rasoolzadeh, 3 Mohammad Reza Zinatizadeh, 3 Peyman Kheirandish Zarandi, 3 Vahid Marmari 2 1Cancer Research Center, Cancer ...
Dana H, Mahmoodi Chalbatani G, Gharagouzloo E, Miri SR, Memari F, Rasoolzadeh R, Zinatizadeh MR, Kheirandish Zarandi P, Marmari V   +8 more
doaj  

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR. [PDF]

PLoS ONE, 2017
The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental ...
Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang   +2 more
doaj   +1 more source