Results 61 to 70 of about 759,664 (294)

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

open access: yesBMC Biotechnology, 2011
Background Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. Results Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any ...
Lu Jia   +5 more
doaj   +1 more source

Single‐cell insights into the role of T cells in B‐cell malignancies

open access: yesFEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

Characterization of Arabidopsis thaliana CPR5 via the elucidation of interacting protein partners : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Biochemistry at Massey University, Palmerston North, New Zealand [PDF]

open access: yes, 2015
he Arabidopsis thaliana Constitutive expresser of pathogenesis related genes5 (CPR5) has previously been suggested to play a role in the regulation of disease resistance, plant and cell proliferation, development and death.
Chiem, Fiona (Shane)
core  

Identification of a non-mammalian leptin-like gene:characterization and expression in the tiger salamander (Ambystoma tigrinum) [PDF]

open access: yes, 2006
Leptin is well established as a multifunctional cytokine in mammals. However, little is known about the evolution of the leptin gene in other vertebrates. A recently published set of ESTs from the tiger salamander (Ambystoma tigrinum) contains a sequence
Boswell, Timothy   +5 more
core   +1 more source

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

open access: yesFEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi   +5 more
wiley   +1 more source

The Carcinoembryonic Antigen Gene Family [PDF]

open access: yes, 1988
The molecular cloning of carcinoembryonic antigen (CEA) and several cross-reacting antigens reveals a basic domain structure for the whole family, which shows structural similarities to the immunoglobulin superfamily.
Thompson, John A., Zimmermann, Wolfgang
core  

Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli [PDF]

open access: yes, 2017
peer-reviewedThe bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin ...
Cotter, Paul D.   +5 more
core   +2 more sources

Goodbye flat lymphoma biology

open access: yesFEBS Letters, EarlyView.
Three‐dimensional (3D) biological systems have become key tools in lymphoma research, offering reliable in vitro and ex vivo platforms to explore pathogenesis and support precision medicine. This review highlights current 3D non‐Hodgkin lymphoma models, detailing their features, advantages, and limitations, and provides a broad perspective on future ...
Carla Faria   +3 more
wiley   +1 more source

Carcinoembryonic Antigen Gene Family [PDF]

open access: yes, 1991
The carcinoembryonic antigen (CEA) gene family belongs to the immunoglobulin supergene family and can be divided into two main subgroups based on sequence comparisons.
Accili   +236 more
core   +1 more source

Mycobacterium tuberculosis sulfurtransferase SseA is activated by its neighboring gene product Rv3284

open access: yesFEBS Letters, EarlyView.
Tuberculosis remains a global health challenge and new therapeutic targets are required. Here, we characterized SseA, a sulfurtransferase from Mycobacterium tuberculosis involved in macrophage infection, and its interaction with the newly identified protein SufEMtb that activates SseA enzymatic activity.
Giulia Di Napoli   +10 more
wiley   +1 more source

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