Results 111 to 120 of about 1,786,363 (358)

Lipoproteins of Mycobacterium tuberculosis : an abundant and functionally diverse class of cell envelope components [PDF]

, 2004
Mycobacterium tuberculosis remains the predominant bacterial scourge of mankind. Understanding of its biology and pathogenicity has been greatly advanced by the determination of whole genome sequences for this organism.
Harrington, Dean, Sutcliffe, Iain
core   +1 more source

Bio‐Orthogonally Crosslinked Supramolecular Polymer Bottlebrush Hydrogels for Long‐Term 3D Cell Culture

Advanced Functional Materials, EarlyView.
Fibrous benzenetrispeptide (BTP) hydrogels, fabricated via strain‐promoted azide‐alkyne cycloaddition (SPAAC) crosslinking, form robust, bioinert networks. These hydrogels can support 3D cell culture, where cell viability and colony growth depend on the fiber content.
Ceren C. Pihlamagi, David Pretzel, Peer Nölte, Kourosh Rezaei, Ulrich S. Schubert, Johannes C. Brendel   +5 more
wiley   +1 more source

Cloning, expression, and purification of recombinant Enterotoxin B and cholera toxin B fusion protein in Lactobacillus plantarum [PDF]

Medical Laboratory Journal
Background: Lactic acid bacteria (LAB) are promising platforms for mucosal vaccine development. Staphylococcus aureus enterotoxin B (SEB), a potent superantigen, is associated with food poisoning and toxic shock syndrome.
Fatemeh Maghsood Ahmadi, Arash Mahboubi, Farzaneh Hosseini, Davoud Esmaeili, Bahareh Hajikhani   +4 more
doaj  

Proteins, Processing, and Properties of Adhesive Fluid Condensates Purified from Mussels

Advanced Functional Materials, EarlyView.
Mussels exhibit an unmatched proficiency for adhering to wet surfaces in salty environments—a remarkable ability that could inspire new biomedical and technical glues. The fluid protein condensates used to form the underwater mussel glue are extracted, reconstituted and characterized with advanced spectroscopy and nanomechanical analysis, revealing ...
Mathieu D. Rivard, Max Renner‐Rao, Nahid Hassanpour, Franziska Jehle, Chang‐Sheng Wang, Duy Anh Pham, Daniel J. Jackson, Xavier Banquy, Matthew J. Harrington   +8 more
wiley   +1 more source

Advanced Protocol for Molecular Characterization of Viral Genome in Fission Yeast (Schizosaccharomyces pombe)

Pathogens
Fission yeast, a single-cell eukaryotic organism, shares many fundamental cellular processes with higher eukaryotes, including gene transcription and regulation, cell cycle regulation, vesicular transport and membrane trafficking, and cell death ...
Jiantao Zhang, Zsigmond Benko, Chenyu Zhang, Richard Y. Zhao   +3 more
doaj   +1 more source

Poly(lactic‐co‐glycolic acid) Nanoparticles for IL‐12 Self‐Amplifying RNA Delivery in Glioblastoma Models

Advanced Functional Materials, EarlyView.
xxxx. ABSTRACT Glioblastoma (GBM) remains one of the most lethal brain cancers, with median survival rarely exceeding 15 months after diagnosis. Interleukin‐12 (IL‐12) is a potent immunostimulatory cytokine capable of reshaping the tumor microenvironment (TME), yet its clinical translation is hindered by systemic toxicity and short half‐life. RNA‐based
Fatima Hameedat, Johan Sebastian Lopez‐Parra, Willem M. H. Hoogaars, Emma Dijkstra, Cynthia Huang, Irafasha C. Casmil, Anna K. Blakney, Peter Olinga, Barbara Rothen‐Rutishauser, Hélder A. Santos, Alke Petri‐Fink, Flávia Sousa   +11 more
wiley   +1 more source

Regulation of virulence in Francisella tularensis by small non-coding RNAs [PDF]

, 2011
Using a cDNA cloning and sequencing approach we have shown that Francisella tularensis expresses homologues of several small RNAs (sRNAs) that are well-conserved among diverse bacteria.
Alain Charbit, Eric Frapy, Guillaume Postic, Iharilalao Dubail, Jonathan Livny, Karin L. Meibom, Marion Dupuis   +6 more
core   +2 more sources

Amyloidogenic Peptide Fragments Designed From Bacterial Collagen‐like Proteins Form Hydrogel

Advanced Functional Materials, EarlyView.
This study identified amyloidogenic sequence motifs in bacterial collagen‐like proteins and exploited these to design peptides that self‐assemble into β‐sheet fibers and form hydrogels. One hydrogel supported healthy fibroblast growth, showing promise for biocompatible materials. Our work demonstrates that bacterial sequences can be harnessed to create
Vamika Sagar, Xuen J Ng, Lukas Lechel, Ashish Chand Rajwar, Hendrik Bargel, Abhishek A. Jalan   +5 more
wiley   +1 more source

Generation of an Induced Pluripotent Stem Cell‐Derived Alveolar Type II In Vitro Model to Study Influenza A Virus Infection and Drug Treatments

Advanced Healthcare Materials, EarlyView.
Patient‐specific induced pluripotent stem cells (iPSCs) can be differentiated into alveolar type II cells (iAT2s), expanded as 3D alveolospheres, and grown at physiologically relevant air–liquid interface (ALI). This study shows for the first time the infectability of iAT2s by the influenza A virus (IAV) and proves their responsiveness to the well ...
Lena Gauthier, Hristina Koceva, Yann Bachelot, Rosanne W. Koutstaal, Puck B. van Kasteren, Marc Thilo Figge, Christian Eggeling, Alexander Sandy Mosig   +7 more
wiley   +1 more source

A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology

Microbial Biotechnology, 2019
Summary The oleaginous yeast Yarrowia lipolytica is an established host for the bio‐based production of valuable compounds and an organism for which many genetic tools have been developed. However, to properly engineer Y.
Macarena Larroude, Young‐Kyoung Park, Paul Soudier, Monika Kubiak, Jean‐Marc Nicaud, Tristan Rossignol   +5 more
doaj   +1 more source