Rapid one-step CRISPR-cas vector assembly by isothermal spacer removal linearization and sequence-ligation independent cloning (ISRL-SLIC). [PDF]
Madsen CK +3 more
europepmc +1 more source
In- & Out-Cloning: plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly. [PDF]
de Vries ST +3 more
europepmc +1 more source
Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable. [PDF]
Chan DTC, Bjerg J, Bernstein HC.
europepmc +1 more source
Abstract Premise As the sister clade to angiosperms, extant gymnosperms are crucial for reconstructing ancestral gene regulatory networks in seed plants. This highlights the need for model systems representing each of their distinct lineages. However, tools to quickly and effectively investigate gene function in gymnosperms are still limited due to the
Anthony G. K. Garcia +4 more
wiley +1 more source
Designing a Vaccine for the Monkeypox Virus Using Immunoinformatics and Structural Tools. [PDF]
Dowran R +4 more
europepmc +1 more source
Abstract A subadult Moschognathus whaitsi from the Eastern Cape Province, South Africa, was scanned using synchrotron radiation X‐ray computed tomography (SRXCT). Its subadult state allowed the cranial bones and teeth to be identified and individually reconstructed in 3D.
Tristen Lafferty +3 more
wiley +1 more source
Conserving genetic material and even increasing genetic diversity is critical. To conduct the conservation of wild boar germplasm resources, we have successfully obtained healthy cloned wild boars for the first time using interspecies somatic cell nuclear transfer and established transgene‐free iPSCs that can be used to conduct iterative rounds of gene
Chen Gao +11 more
wiley +1 more source
A composition-matching algorithm, MatchIDR, identifies prion-like domains that localize to stress granules. [PDF]
Cascarina SM, Paul KR, Ford LL, Ross ED.
europepmc +1 more source
Bioprospecting Ulleungdo‐isolated microalgae for carbon valorization and utilization
Abstract The growing demand for renewable energy alternatives has intensified global interest in microalgae as versatile bioresources for carbon valorization and bioenergy production. Owing to their rapid growth and biochemical versatility, microalgae provide a sustainable route for converting captured carbon into bioenergy and high‐value products ...
Ha‐Neul Choi +5 more
wiley +1 more source
Metagenomic analysis of the biofilm community at the oxic-anoxic interface of a deep-underground saline spring at the Baksan Neutrino Observatory. [PDF]
Tarasov K +4 more
europepmc +1 more source

