Results 181 to 190 of about 1,949,061 (374)

Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66 [PDF]

, 1995
Michael J. Butler, Craig Binnie, Michele A. DiZonno, Phyllis Krygsman, Glenn Soltes, Gisela Soostmeyer, Eva Walczyk, Lawrence T. Malek   +7 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

InSillyClo, a User-Friendly Web Application to Assist Large-Scale Golden Gate Cloning and MoClo Workflows. [PDF]

ACS Synth Biol
Galez H, Brancotte B, Bonche J, Fumey J, Napolitano S, Batt G.   +5 more
europepmc   +1 more source

Cloning conundrums [PDF]

Nature Medicine, 2002
openaire   +2 more sources

Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemia

, 1998
Alison Brownlie, Adriana Donovan, Stephen J. P. Pratt, Barry H. Paw, Andrew C. Oates, Carlo Brugnara, H. Ewa Witkowska, Shigeru Sassa, Leonard I. Zon   +8 more
openalex   +2 more sources

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

CrisprBuildr: an open-source application for CRISPR-mediated genome engineering in Drosophila melanogaster. [PDF]

G3 (Bethesda)
Horsley N, Sythoff AVB, Delgado M, Liu S, Cabernard C.   +4 more
europepmc   +1 more source

SELECTION OF EUCALYPT CLONES WITH HIGHER STABILITY IN PULP YIELD [PDF]

, 2020
Thaise da Silva Souza, Bruno Marco de Lima, José Luís Lima, Aurélio Mendes Aguiar, Donizete da Costa Dias, Gabriel Dehon Sampaio Peçanha Rezende, Magno Antônio Patto Ramalho   +6 more
openalex   +1 more source

Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276

FEBS Open Bio, EarlyView.
Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig, Swanhild Lohse, Sara Elahi, Nils Hartmann, Stefanie Deckert, Shutian Si, Alexander Desuki, Anja Harder   +7 more
wiley   +1 more source

CloneCoordinate: Open-Source Software for Collaborative DNA Construction. [PDF]

ACS Synth Biol
Jeon E, Shen Z, Christ S, Qi E, Fan I, Lee N, Morgan O, Ohl M, Millson D, Laker M, Pierson A, Villegas Garcia E, Zhang Y, Choi A, Iyengar A, Kim R, Lee J, Niedeck L, Oeien V, Rashid M, Seetharaman N, Singh A, Soble D, Yu J, Yu K, Berdy S, Chang E, Kitazono R, Ortiz S, Taylor D, Thuronyi B.   +30 more
europepmc   +1 more source