Evaluation of a new cytotoxicity assay forClostridium perfringenstype D epsilon toxin [PDF]
A new cytotoxicity assay for determining the activity of epsilon toxin produced by Clostridium perfringens type D has been developed. Viability of cultured cells was determined by the ability of only live cells to convert 5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4- sulfophenyl)tetrazolium to the coloured product formazan in the presence ...
D W, Payne +4 more
openaire +2 more sources
Molecular Detection of Clostridium perfringens Type D Alpha and Epsilon Toxin Genes from Various Tissues in Lambs [PDF]
Different tissues including duodenal scrapings, liver, lungs and kidneys of each infected (n=6) and control (n=4) lambs were subjected to PCR for the identification of alpha and epsilon toxin genes of Clostridium perfringens type D.
Azam Ali Nasir1,3, Muhammad Younus2,*, Muti-ur-Rehman3, Muhammad Latif3, Asif Rashid1, Rashid Ahmad1 and Muhammad Abbas1
doaj
Clostridium perfringens toxinotype D, toxinotype E, and gastroenteritis-linked BEC/CPILE-positive strains have never been reported in healthy children. We isolated, whole-genome sequenced and bioinformatically characterised three C. perfringens isolates
Raymond Kiu +6 more
doaj +1 more source
Further characterization of Clostridium perfringens small acid soluble protein-4 (Ssp4) properties and expression. [PDF]
Clostridium perfringens type A food poisoning (FP) is usually caused by C. perfringens type A strains that carry a chromosomal enterotoxin gene (cpe) and produce spores with exceptional resistance against heat and nitrites.
Jihong Li +3 more
doaj +1 more source
Changes of Microbial and Component Composition of Giraffe Milk During Mid‐ to Late Lactation
Giraffe milk – graphical abstract text, Giraffe: late lactation milk, Involution at approximately 15 months of lactation, Content of saccharides and fat decrease, Protein content increases, Content of minerals increase, Microbiological quality high ABSTRACT The proximate, mineral and microbiological composition of milk from five giraffes from mid ...
G. Osthoff +4 more
wiley +1 more source
Antagonistic Effect of Intestinal Bacteria from the Microflora of Holoxenic (Conventional) Piglets, Against Clostridium Perfringens in the Digestive Tract of Gnotoxenic Mice and Gnotoxenic Piglets [PDF]
Antagonistic effect of piglet microflora against Clostridium perfringens was studied in germfree mice, to isolate bacterial strains responsible for this colonization resistance.
Nicolas, Jean-Louis, Corpet, Denis E.
core
Detection of zoonotic bacteria in wild Australian freshwater turtles
Freshwater turtles are long‐lived, abundant components of Australian aquatic ecosystems, yet little is known about the diversity and prevalence of zoonotic bacteria they may carry. In this study, we conducted one of the most comprehensive surveys to date of zoonotic microorganisms associated with wild Australian freshwater turtles.
TF Scheelings +3 more
wiley +1 more source
O grande impacto do Clostridium perfringens na avicultura
Clostridium perfringens tipo A é uma bactéria Gram-positiva anaeróbia que habita a biota intestinal nativa do intestino grosso de aves, causando uma enterotoxemia necrótica, especialmente em sistemas intensivos de frangos de corte com mais de 4 semanas ...
Lina Maria Peñuela +3 more
core +1 more source
ABSTRACT Background Associations between microbial dysbiosis and malignancies of the pancreatobiliary system have been described in recent studies. As a result of the limited number of studies that have been done specifically on the malignancies of the biliary tract, information regarding oral, biliary and tumour‐related microbial alterations was ...
Esperouz Fariba +5 more
wiley +1 more source
Intramural Vascular Edema in the Brain of Goats With Clostridium perfringens Type D Enterotoxemia [PDF]
Enterotoxemia caused by Clostridium perfringens type D is an important disease of sheep and goats with a worldwide distribution. Cerebral microangiopathy is considered pathognomonic for ovine enterotoxemia and is seen in most cases of the disorder in sheep. However, these lesions are poorly described in goats.
Joaquín Ortega +5 more
openaire +2 more sources

